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Where r is the volumetric ratio of reverse micellar phase to aqueous phase Vrm/Vaq. KPRM and KRM are equilibrium constants [S] is surfactant concentration m is aggregation number n is number of rvms needed to form rvms-protein complex
Few surfactants are able to establish stable water-in-oil micro emulsions without the so called co-surfactant, another surface active additive. Surfactants, that do not need a co-surfactant, include anionic AOT The organic solvent used influences the aggregation behavior, the reversed micelle size and shape as well as the interdroplet interactions.