Actinomycetes Metalloprotease Streptomyces olivochromogenes Ion exchange Poros HQ Gel filtration Sepharose CL-6B Gel electrophoresis Gelatine Zymography

Phenyl Methyl Sulfonyl Flouride Aprotinin Pefabloc SC Insatiably increasing Microbial Protease Tannery Laundry detergent Feather digestion Aqueous condition Non aqueous Media Molecular memory Natural aqueous media Physical & chemical method Chemical modification Immobilization Protein engineering Medium engineering Extracellular protease Alkaline environment PH, Alkaline PH Surfactant or oxidizing agent Subtilish carlsberg Subtilisin Novo Savinase, Esperase alcalase Modern bleach based detergent ingredient Nonionic surfactant Anionic surfactant Phospholipase D Strain Profound proteolytic activity Azocasein Folin-ciocaltens phenol reagent Tyrosine Analytical grade reagent Bacterial Strain Oat meal Bovine serum albumin Standard protein mM Aliquots 0.06 mL 10% (W/V)trichloroacetic acid

Centrifugation Supernatant UV-1601 Spectrophotometer Dialyzed Ultrafiltration membrane Dialysate Pre-equilibrated Matrix-bound proteins Eluted Laemmli Low molecular weight marker Coomassie brilliant blue R-250 Destained Zymography Heussen & dowdle mA Triton X-100 Destaining Eppendrof Tubes Parafilm Rotary shaker Lineweaver-burke plot Anion exchange chromatography Size exclusion chromatography Elution Metalloendopeptidase Bioremediation