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  • Lysis Buffer

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    Janak Thakkar
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    LYSIS BUFFER: 3. MM sodium dihydrogen phosphate 50 mM disodium hydrogen phosphate 47. Mm sodium chloride, 5 mM potassium chloride, 61 mM glucose, 0.1% Triton x-100 and 0. MM EDTA. Lysate solution: freshly prepared 2% potassium ferric cyanide and 2% ferric chloride in water 10% acetic acid and 5% methanol.

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    LYSIS BUFFER: 3. MM sodium dihydrogen phosphate 50 mM disodium hydrogen phosphate 47. Mm sodium chloride, 5 mM potassium chloride, 61 mM glucose, 0.1% Triton x-100 and 0. MM EDTA. Lysate solution: freshly prepared 2% potassium ferric cyanide and 2% ferric chloride in water 10% acetic acid and 5% methanol.

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    42 views1 page

    Lysis Buffer

    Uploaded by

    Janak Thakkar
    LYSIS BUFFER: 3. MM sodium dihydrogen phosphate 50 mM disodium hydrogen phosphate 47. Mm sodium chloride, 5 mM potassium chloride, 61 mM glucose, 0.1% Triton x-100 and 0. MM EDTA. Lysate solution: freshly prepared 2% potassium ferric cyanide and 2% ferric chloride in water 10% acetic acid and 5% methanol.

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    LYSIS BUFFER: 3.35 mM sodium dihydrogen phosphate 50 mM disodium hydrogen phosphate 47.

    8 mM sodium chloride, 5 mM potassium chloride, 61 mM glucose, 0.1% Triton X-100 and 0.1 mM EDTA. SOD: 25 ml of 3 mM nitro blue tetrazolium solution 30 ml of 0.1 M potassium phosphate buffer (pH 7.0) Containing 28 lM riboflavin and 28 mM TEMED CATALASE: 5% methanol Distilled water; 30 ml of 5 mM H2O2. 1:1 mixture of freshly prepared 2% potassium ferric cyanide and 2% ferric chloride in water 10% acetic acid and 5% methanol GPx: 50 mM TrisHCl buffer (pH 7.9) Substrate solution (50 mM TrisHCl buffer, pH 7.9, 13 mM GSH, and 0.008% hydrogen peroxide)

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