ng Dng Mi Trong Nhn Ging Lan H ip

GVHD: L Th Thy Tin

NG DNG MI TRONG NHN GING

LAN H IP
YC LC
PHN I

TNG QUAN

CHNG I

S Lc V Lan H ip

1.1

Gii thiu v lan H ip.

1.1.1.

V tr loi.

1.1.3.

Ngun gc phn b.

1.1.1.

iu kin sinh trng v pht trin.

1.2.

Gi tr kinh t.

Trong nc.

Th gii.

1.2.1.
1.2.2.
1.3.

Lch s pht trin ca phng php nui cy m thc vt.

CHNG 2



CC PHNG PHP NHN GING



2.1.

ging bng phng php truyn thng.



2.1.1.

Nhn Phng php gim cnh.



2.1.2.

Nhn ging bng phng php ghp cnh.



2.1.3.

phng php chit cnh



2.1.4.

Phng php gieo ht.



2.1.5.

Nhn xt.



2.2.

Nhn ging bng phng php hin i.



2.2.1. Cc giai on ca qu trnh vi nhn ging.



2.2.4.

Phng php nui cy nh sinh trng.

2.2.4. Nhn ging bng to ht nhn to.

2.3.



Nhn xt.







NG DNG MI VO NHN GING



LAN H IP



CHNG 3



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ng Dng Mi Trong Nhn Ging Lan H ip

GVHD: L Th Thy Tin
C U NG DNG H THNG NUICY NGP CHM TM THI TRONG NHN GING
LAN H IP LAI PHALAENOPSIS HYRID

3.1.

H thng ngp chm.



3.1.1.

S lc.



3.1.2.

Cc h thng ngp chm.



3.1.2.1.

H thng RITA



3.1.2.2.

H thng bnh sinh i IT .



3.1.2.3.

H thng Plantima.



3.2.

VT LIU PHNG PHP



3.2.1.

H thng nui cy:



3.2.2.

Mu cy Lan H ip



3.2.3.

Phng php th nghim:



3.3.

Kt qu.



3.3.1.

Kho st s nhn nhanh PLs trong cc h thng nui cy khc nhau



3.3.2.

Kho st s ti sinh chi t PLs trong h thng TIS

3.3.3.

Kho st s ra r ca chi H ip ti sinh t PLs trong h thng TIS



[ 


L


P  V PROTOCORM-L BODY C
Y

 P B  T
BORCTOR




4.1. S lc h thng IOREACTOR.



4.2. VT LIU V PHNG PHP



4.2.1. Vt liu



4.2.2. iu kin phng nui cy



4.2.3. Phng php th nghim



4.3.THIT K TH NGHIM



4.3.1.Ti u ha mi trng tng sinh phi v PL



4.3.2. Kho st s tng sinh ca phi v PL trong h thng bioreactor

4.3.3. X l s liu

4.4.KT QU

4.4.1. Xc nh mi trng ti u tng sinh phi v PL

4.4.2. nh hng ca h thng nui cy bioreactor ln s tng sinh phi v PL



4.5. K LUN

[




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 C CC MOOSCC RD V DSCC RD L QU TR P T
S P  V T C
Y L  P (P.AMAILIS)


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GVHD: L Th Thy Tin
5.1. NH HNG CA NGUN CARON



5.2. VT LIU V PHNG PHP



5.2.1. Mu cy



5.2.2. Mi trng nui cy



5.2.3. iu kin th nghim



5.2.4. Thit k th nghim



5.2.5. Phn tch s liu



5.3. KT QU



5.3.1. nh hng ca cc monosaccharide ln s pht sinh phi v tnh.



5.3.2. nh hng ca disaccharide ln s pht sinh phi v tnh

5.4. KT LUN



TI LIU THAM KHO



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GVHD:TS. L Th Thy Tin

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SVTH: Lu Vn Ton
 

PHN II: NG DNG MI VO NHN GING LAN H IP

Chuong 3: c u ng Dng H Thng Nui

Cy Ngp Chm Tm Thi Trong Nhn Ging
Lan H ip Lai Phalanopsis hybrid

! Kho st s ti sinh chi t PLs trong h thng TIS

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3.3. Kt qu.
3.3.1. Kho st s nhn nhanh PLs trong cc h thng nui cy khc nhau
3.3.1.1. Ti u qu trnh nhn nhanh PLs trong h thng TIS
[{
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GVHD:TS. L Th Thy Tin

27

SVTH: Lu Vn Ton
 

PHN II: NG DNG MI VO NHN GING LAN H IP

Chuong 3: c u ng Dng H Thng Nui

Cy Ngp Chm Tm Thi Trong Nhn Ging
Lan H ip Lai Phalanopsis hybrid

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GVHD:TS. L Th Thy Tin

28

SVTH: Lu Vn Ton
 

PHN II: NG DNG MI VO NHN GING LAN H IP

Chuong 3: c u ng Dng H Thng Nui

Cy Ngp Chm Tm Thi Trong Nhn Ging
Lan H ip Lai Phalanopsis hybrid

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Hnh 11. a: PLs c nui trong h thng platima tun sut 2 gi/5 pht

3.3.1.2.Kho st s nhn nhanh PLs trong cc h thng nui cy khc nhau

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Hnh 12: PLs c nui cy trong cc loi h thng khc nhau:
a: Lng c lc; b: trn TIS; c: trn thch

GVHD:TS. L Th Thy Tin

29

SVTH: Lu Vn Ton
 

PHN II: NG DNG MI VO NHN GING LAN H IP

Chuong 3: c u ng Dng H Thng Nui

Cy Ngp Chm Tm Thi Trong Nhn Ging
Lan H ip Lai Phalanopsis hybrid

3.3.2. Kho st s ti sinh chi t PLs trong h thng TIS

3.3.2.1.Ti u mt PLs ln s ti sinh chi v nhn chi trong h thng
TIS
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GVHD:TS. L Th Thy Tin

30

SVTH: Lu Vn Ton
 

PHN II: NG DNG MI VO NHN GING LAN H IP

Chuong 3: c u ng Dng H Thng Nui

Cy Ngp Chm Tm Thi Trong Nhn Ging
Lan H ip Lai Phalanopsis hybrid

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GVHD:TS. L Th Thy Tin

31

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Chuong 3: c u ng Dng H Thng Nui

Cy Ngp Chm Tm Thi Trong Nhn Ging
Lan H ip Lai Phalanopsis hybrid

Kt qu
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GVHD:TS. L Th Thy Tin

33

SVTH: Lu Vn Ton
 

PHN II: NG DNG MI VO NHN GING LAN H IP

Chuong 3: c u ng Dng H Thng Nui

Cy Ngp Chm Tm Thi Trong Nhn Ging
Lan H ip Lai Phalanopsis hybrid

3.4.
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GVHD:TS. L Th Thy Tin

34

SVTH: Lu Vn Ton
 

PHN 2:

Chng 5: nh Hng Ca Cc Monosaccharide V

Disaccharide Ln Qu trnh Pht Tri n Phi
V Tnh Cy Lan H ip (P. amabilis)

[ 
NHN NHANH PHI V PROTOCORM-LIKE ODY CY
LAN H IP >NG H THNG IOREACTOR
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GVHD:TS. L Th Thy Tin

35

SVTH: Lu Vn Ton
 

PHN 2:

Chng 5: nh Hng Ca Cc Monosaccharide V

Disaccharide Ln Qu trnh Pht Tri n Phi
V Tnh Cy Lan H ip (P. amabilis)



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GVHD:TS. L Th Thy Tin

36

SVTH: Lu Vn Ton
 

1
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Chng 5: nh Hng Ca Cc Monosaccharide V

Disaccharide Ln Qu trnh Pht Tri n Phi
V Tnh Cy Lan H ip (P. amabilis)

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GVHD:TS. L Th Thy Tin

38

SVTH: Lu Vn Ton
 

PHN 2:

Chng 5: nh Hng Ca Cc Monosaccharide V

Disaccharide Ln Qu trnh Pht Tri n Phi
V Tnh Cy Lan H ip (P. amabilis)

ng 12.[5?A@>A#4 

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Mi trng nui cy

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GVHD:TS. L Th Thy Tin

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GVHD:TS. L Th Thy Tin

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PHN 2:

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GVHD:TS. L Th Thy Tin

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PHN 2:

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GVHD:TS. L Th Thy Tin

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GVHD:TS. L Th Thy Tin

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SVTH: Lu Vn Ton
 

PHN 2:

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52

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53

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54

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GVHD:TS. L Th Thy Tin

55

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56

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GVHD:TS. L Th Thy Tin

56

SVTH: Lu Vn Ton