PH

NG PHP XC

NH

MELAMINE TRONG S A
GVHD: Phng V C m H ng SVTH: L Th M Trang L p : DH07HH MSSV: 07139234

T NG QUAN MELAMIN
L m t bazo h u c t tan trong n c CTPT: C3H6N6 M = 126 Danh php:1,3,5-triazine-2,4,6-triamine
6 (NH2)2CO
Ure

C3H6N6 + 6 NH3 + 3 CO2

Melamine

MELAMINE

ng d ng c a Melamine:

L thnh ph n chnh c a CNSX Cc v t d ng gia nh (chn a). formica (dng trong cng ngh bn gh , t m vch...). Bao b. Nh a melamine dng lm ch t keo dn... L m t HCCN c s d ng trong ch t d o, phn bn, qu n o ch ng chy, thu c nhu m, h v nhi u v t gia d ng khc.

PHN TCH HM L NG MELAMIN TRONG S A

PHN TCH HM L

NG MELAMINE TRONG S A

1.

it

ng p d ng:
S ab t S a l ng

PHN TCH HM L

NG MELAMINE TRONG S A

2. D ng c v thi t b :
Cn phn tch ( chnh xc n mg) ng ly tm 15 ml Bnh nh m c 10ml, 20ml Pipet 1ml,10ml

My ly tm B siu m
H th ng my s c k l ng co p UltiMate 3000 HPLC/UV

PHN TCH HM L

NG MELAMINE TRONG S A

3. Ha ch t:
Water, from Milli-Q Gradient A 10. Methanol (CH3OH), HPLC grade, Fisher. Acetonitrile (CH3CN), HPLC grade, Fisher Ammonium acetate (NH4Ac), analytical grade, SCRC, China. Acetic acid (HAc), analytical grade, SCRC, China Sodium 1-octane sulfate (98%), Baker Analyzed HPLC Reagent, USA. Melamine (99.0%), HPLC grade, Fluka.

D ng ng chu n: Cn 100mg melanine+dd methanol (50%,v/v) vo bnh nh m c 100ml N ng melamine 1000ppb Pha chu n v i cc n ng :

0.05, 0.1, 0.2, 1.0, 5.0, 20, and 40 ppb

4. Chu n b m u:
a. S a b t:
Cn 1g m u

PHN TCH HM L

NG MELAMINE TRONG S A

c s y kh + 10ml n L c ha tan B siu m

c
(1ph)

ng ly tm 15ml

(30ph)

1 ml HAc (3%)

Ha tan 4oC, 30ph

Mu Ly tm
(15ph) Ph n n c

H2O

Bnh M 10ml L c qua a 0,2 m

PHN TCH HM L

NG MELAMINE TRONG S A

4. Chu n b m u:
b. S a l ng:
10 ml s a + 1ml HAc (3%)
4oC, 30ph

ng ly tm 15ml

Ly tm
H2O

(15ph) Ph n n c

Bnh M 10ml L c qua a 0,2 m

PHN TCH HM L

NG MELAMINE TRONG S A

4. Chu n b m u:
c. S a b t + s a l ng (spiked sample): 1g s a b t (s y) + 10ml s a l ng + 0,04 ml melamin L c ha tan
Ha tan 4oC, 30ph

ng LT 15ml

(1ph) (30ph)

B siu m Mu Ly tm

1 ml HAc (3%)

(15ph) Ph n n c

H2O

Bnh M 10ml L c qua a 0,2 m

PHN TCH HM L

NG MELAMINE TRONG S A

5. i u ki n ch y my
C t b o v : Acclaim Mixed-Mode WCX-1, 5 m, 4.3 10mm, P/N 068354, with guard column holder, P/N 59526 C t phn tch: Acclaim Mixed-Mode WCX-1, 5 m, 4.6 250 mm, P/N 068352 Nhi t c t : 30 C Pha ng : ch t m Acetate (mixture of 700 mL of 10mM HAc and 300 mL of 10 mM NH4Ac, ~ pH 4.3) CH3CN (8 : 2, v/v) T c dng: 1.0 mL/min Inj. Volume: 20 L UV detection: Absorbance at 240 nm

PHN TCH HM L

NG MELAMINE TRONG S A

PHN TCH HM L

NG MELAMINE TRONG S A

S a b t: Sample#1 -> sample#3 S a l ng: Sample#4,#5