Enzymes 1.

Explain how enzymes are able to catalyse biological reactions

2. What is meant by the specificity of enzymes?

3. How does the lock and key hypothesis different from the lock and key hypothesis?

4. Draw two graphs to show the effect of temperature and substrate concentration on the activity of an enzyme.

5. Explain the effect of temperature over the region marked A 6. Name the temperature marked B. Explain your choice. 7. What is happening to the enzyme at temperatures marked C? Why?

8. Explain the effect on enzyme activity of increasing the substrate concentration over the region marked X. 9. Why is there no change in the activity of the enzyme when the substrate concentration increases over the region marked Y? 10. Complete the following paragraph. Enzymes have a region called the ___________________, which has a specific shape. Substrate molecules have a shape, which is ___________________ to this and so can bind with it to form an ___________________. Some inhibitor molecules have a shape similar to the substrate. These are called ___________________ inhibitors. They can fit into the ___________________ of the enzyme and block it. A higher proportion of inhibitor to substrate causes ___________________ inhibition. Other inhibitors bind to a different part of the enzyme called the ___________________. This changes the ___________________ of the ___________________ so that the substrate cannot bind. This inhibitor is called a ___________________ inhibitor. This inhibition is ___________________ of substrate concentration. 11. Draw the graph that shows the effect of an inhibitor on the activity of an enzyme. Draw one for a competitive inhibitor and another for a non-competitive inhibitor.