Cng ho x hi ch ngha Vit nam

B Nng nghip v pht trin nng thn

_________________________

Tiu chun ngnh 10TCN 454-2001

Phn tch cy trng

Phng php xc nh kali tng s v natri tng s

H ni 2001

T chc chu trch nhim bin son tiu chun: Vin Th nhng Nng ho C quan ngh ban hnh tiu chun: V Khoa hc cng ngh v CLSP C quan ban hnh tiu chun: B Nng nghip v Pht trin Nng thn

Nhm C

Tiu chun ngnh 2001

10 TCN 454-

Phn tch cy trng

Phng php xc nh kali tng s v natri tng s

1. Phm vi p dng. Tiu chun ny quy nh phng php xc nh kali tng s, natri tng s bng phng php quang k ngn la, p dng cho tt c cc mu cy trng. 2. Nguyn tc. Ho tan cc dng kali v natri ca cy theo 10TCN 4502001. Xc nh hm lng K, Na trong dung dch bng quang k ngn la. 3. Thit b v thuc th 3.1. Thit b 3.1.1. Thit b phn hu mu theo 10TCN 450-2001 3.1.2. Quang k ngn la 3.1.3. Bnh nh mc 500ml, 1000ml 3.1.4. Cn phn tch c chnh xc 0,0002g 3.2. Thuc th 3.2.1. Dung dch kali tiu chun 1000ppm K Cn 1,9067g KCl tinh khit sy kh 110oC. Ho tan bng nc nh mc n 1000ml, dung dch ny c nng 1000ppm K, pha long 10 ln c dung dch 100ppmK dng chun b cc dy tiu chun xc nh K. 3.2.2. Dung dch tiu chun natri 1000ppm Na Cn 1,8482g NaNO3 tinh khit sy kh 110oC. Ho tan bng nc nh mc n 1000ml, dung dch ny c nng 1000ppm Na, pha long 10 ln c dung dch 100ppmNa dng chun b cc dy tiu chun xc nh Na. 3.2.3. Nc ct c dn in nh hn 2 S/cm, pH 5,6-7,0 4. Cch tin hnh 4.1. Chun b dy tiu chun 4.1.1. Dy tiu chun K: S dng bnh nh mc 50ml cho vo cc bnh theo th t s ml dung dch tiu chun 100ppmK v thm nc cho n vch nh mc theo bng sau:

10TCN 454-2001 Nng ca dung dch K tiu chun (0-50ppm K) 0 5 10 20 30 40 50 S ml dung dch K tiu chun 100ppm 0 2,5 5,0 10,0 15,0 20,0 25,0

4.1.2. Dy tiu chun Na: S dng bnh nh mc 50ml cho vo cc bnh theo th t s ml dung dch tiu chun 100ppm Na v thm nc cho n vch nh mc theo bng sau: Nng ca dung dch Na S ml dung dch Na tiu tiu chun (0-40ppm Na) chun 100ppm 0 0 5 2,5 10 5,0 20 10,0 30 15,0 40 20,0 4.2. Chun b mu: theo 10TCN 449-2001 4.3. o dung dch tiu chun v dung dch mu 4.3.1 o cc dung dch tiu chun trn quang k ngn la vi K ti bc sng 768nm, vi Na ti bc sng 589nm (hoc knh lc tng ng). Lp th tiu chun biu th tng quan ca nng K (hoc Na) trong cc dung dch tiu chun vi s o tng ng trn my. 4.3.2. o dung dch mu trn quang k ngn la ng nht vi iu kin o dy tiu chun. Thng xuyn o kim tra bng cc dung dch tiu chun*

4.3.3. Cn c vo th tiu chun v s o trn my xc nh nng dung dch mu (ppm)

5. Cch tnh kt qu

Cng thc tnh hm lng K (Na) trong mu kh tuyt i nh sau: % K (Na) = a . V. k m . 104
Trong : m : khi lng mu phn hu (g) V: th tch dung dch mu (ml) a: nng nguyn t trong dung dch mu (ppm) k: h s chuyn v kh kit

10TCN 454-2001

Ghi ch:

* C th xc nh K,Na bng AAS ch o pht x hoc ch o hp th s dng n HCL tng ng ti bc sng 766,6 nm vi Kali v 589,0nm vi Na trn ngn la C2H2/KK

** Hiu chnh quang k ngn la hot ng trng thi n nh mi tin hnh o dy tiu chun. Trong qu trnh o mu cn thng xuyn kim tra bng cc dung dch tiu chun v hiu chnh ( t nht 10 mu phi kim tra 1 ln). K thut s dng theo hng dn ca hng sn xut.
*** khc phc nh hng ca Canxi, cn cho thm t l th tch 1:1 dung dch 0,2% Cs + 0,36% Al pha t CsCl v Al(NO3)3 vo dung dch mu.

K/T.b trng B Nng nghip v PTNT Th trng Ng Th Dn : k