Validation and Caliberation of HPTLC Instrument Used : CAMAG HPTLC System with CAMAG TLC Scanner & LINOMAT

5 Applicator. Standard Operating Procedure Describes Preparation of CAMAG Scanner qualification test plate which has to be followed to ensure reliable Optional qualification for following instrument. TLC scanner 2 TLC scanner 3 Prepration: Prepare a test solution containing 100g /ml Ethanolic solution of caffeine for band wise sample application. A HPTLC plate silica gel 20 x 10cm used. Bandwise: Use a Validated linomate or automatic TLC Sampler 3 to apply 2000ml ie; 2ml in a 8mm Band at exposition, 40mm & y position 8mm. Dry application zones b/w preparation and used for testing. This Scanner Qualification test plate must be wrapped with aluminium foil and kept in a cool and dry place for not longer than 2 weeks. HPTLC plate is now chromatographed about 40mm with Toluene-Acetone (9:1) and then dried. Specification Test: To confirm proper preparation , HPTLC plate is now scanned in certified TLC scanner 3 at 275nm using CATS soft. Position the scanner test plate in TLC scanner 3. Oppen the method DQ line. PAM in CAT and create a new analysis data file. Make the plate is properly Positioned and then scan. Start Position X and aligned with fraction to be measured. Now scan, integrate, evaluate plate using new application pattern above. In order to avoid the influence of fluorescent indicator set the integrator start limit(track start) at a position approximately 2mm after this scan starts position Y ie; about 1mm. Calculation is performed in the reproducibility mode.

The resulting Vh (relative standard deviation by peak hight) considers the only measuring error and has to be below 0.25% . Operation Qualification of LINAMAB 5.