2011

1

:

:110125

1.)

2.)

3.)

3.1
3.2
3.3

4.)

4.1
4.2
4.3
4.4

.Vulgare

5.)

5.1 .Vulgare
5.2
in vitro
in vivo
5.3


3
1 . (Origanum Vulgare
sp.).
.
. /
.
( )
.
.

. .

.
.

2.)


. .
3 .( 1)

1 :
2 : E &
3 :

3 : M

GC , HPLC, IR, MS

1 :

2 :

3.)

, , , .
.
5.

3.1) -

Origanum Vulgare
,
1,2,3 ,
4 ,
5 ,
6
7
3

Origanum vulgare hirtum

Origanum vulgare onites
Origanum vulgare syriacum

.Vulgare

6
20-50 cm.

Origanum heracloeticum L, O.hirtum Link ,O.Parviflorum Urv.,

Origanum Vulgare L.,O.viride Hal, O.Vulgare var. viride Boiss
A
Origanum onites L., Majorana onites Benth ,
.
Origanum maru L., Majorana onites Benth .
: , , .
Origanum majorana L, Majorana hortensis Moench
.
riganum dictamus L. ,
.

3.2) -
,
.
.
.
25 C +42 C 18 -22 C.
pH 6 8(6< pH <8.

(,)
.
. 10 max.
.
( )
. .
4

3.3)
Oregano
.
(,,)
.. ,
,
.

( 5 .., 327-287 ..
, 1 . ).
.

. , ,
,, .

1800
20
.
International Trade Center,
1982.

4.)
4.1.)
.
3-5% .
( 80%) ( 15%).

.
3 :

( -, P- ,, )

Origanum Vulgare sp., ssp. hirtum

(-,p-,,)
: O.Vulgare ssp. hirtum
CH2Cl2 12 h 5
: TLC

 4 :

(flavus=)
Origanum Vulgare sp. , ssp. hirtum. Glc= glucose(), GlcA=glucuronic acid
( )
4 (1) ,(2) ,
(3) , (4) epi-
: (5) o ( R1,R2,R3)
(6) ( R1,R2,R3)
6

 1 :
Origanum Vulgare ssp. Hirtum LC-MS
UV HLPC-PDA

( 1)

HPLC ( 80% MeOH)

Origanum Vulgare ssp. hirtum 280 nm 360 nm .
.
/peak numbers
( 1 19 ) 1.

( 1)
8

 : .
,, , , ,.

, .
2 : % O.nites 6 3
.( , , )

06 :00 h ( (total) : 98.84%)

6: E

 6 % 3 .Vulgare(-,
p-,) .

4.2.)
7 :
.
1 ) 7
.
.
.
. .
.
( )
( )
.

.

2)
.
. /
/ .

. .


.
Soxhlet 8.
CO2
.( 9)
8 Soxhlet

9 : CO2
10

4.3)

.
.
.

, ,,,.
.Vulgare .
(10) .Vulgare

.
O.Vulgare () ( 10)

10 :

( 3)

11

4.4) .Vulgare
,
,
, .
( , )
.
.
4 .Vulgare A,B,C,D,E,F 2 GC HPLC
35

3: 4
( : , , , , )

4 : gr , ml
(0.5,0.6 kgr)
ml .

5 : % 4
p- -
.

12

 11 : 4 .Vulgare

5.)
5.1.) .Vulgare

.

,
.
.
.
.

.

.
.
, ,, , ,
.
(4)

5.2) (state of the art)

in vitro
.
. Drosophila melanogaster


.
O.Vulgare . .

.
Listeria Monocytogenes
. .


. , 2005

, .

13

.) in vitro .Vulgare

in vitro

Petri
Petri
. irby & Bauer ().

(Minimum Inhibitory Concentration)
Broth microdilution method.
a) .Vulgare in vitro
6
6
.Vulgare

.
(1.0gr/ml).
mm
. Kirby &
Bauer. 3

.Vulgare.
5
7
7
.Vulgare

. Broth microdilution
(MIC)
.
O.Vulgare
.
.

14

5
3 .

O.Vulgare . Kirby & Bauer.
( ).

(P.Mirabilis,P.aeruginosa,L.monocytogenes) .

L.monocytogenes
-
L.monocytogenes .
.

9
9
.
3 Broth
microdilution.

(MIC).



.
-

.

:



,
in vivo

.

5
15

) in vivo
n vivo O.Vulgare :

A)
.Vulgare

B)

20 mg/kg
6

5.3

, .
( ) .

. 2
(Ecopharm Hellas ).
:

.
,
, .
(GC)

Content and Composition of Volatile Terpenes, Flavonoids and

Phenolic Acids in Greek Oregano (Origanum vulgare L. ssp. hirtum)
at different Development Stages during Cultivation in Cool Temperate Climate
K. Grevsen, X.C. Frett and L.P. Christensen Europ.J.Hort.Sci. 74

S. 193203, 2009, ISSN 1611-4426. Verlag Eugen Ulmer KG, Stuttgart

2 Changes in Essential Oil Composition of Oregano (Origanum onites L.)

due to Diurnal Variations at Different Development Stages
Notulae Botanicae Horti Agrobotanici, Academic Press

3 tlas of Microscopy of medicinal plants and culinary herbs P. JACKSON

Ph.D., B.Pharm., B.Sc. (Lond), F.R.Pharm.S., F.L.S. Belhaven Press,London

16

& W. SNOWDON

4 Intraspecific variability of wild marjoram (Origanum vulgare L.) naturally

occurring in Poland Z. Wglarz E. Osidska A. Geszprych J. Przybyb
5

Chemical Composition and Antibacterial Activity of Essential

Oils of Ten Aromatic Plants against Human Pathogenic Bacteria
Marina Sokovi Petar D. Marin Dejan Brki Leo J. L. D. van Griensven
Food 2007 Global Science Books

a
a

6 WHO (World Health Organisation) monographs on medicinal plants (Vol. 5) commonly used in the

Newly Independent States (NIS)

7

CURRENT SCIENCE, VOL. 98, No. 8, 25 APRIL 2010

P. S. GURUDATT1, Department of Crop Physiology
R. VASUDEVA Department of Forest Biology, College of Forestry, Sirsi, Indi
S. DEEPIKA Natural Products Chemistry Division, Indian Institute of Integrative Medicine

Wikipedia, Origanum Vulgare

17