Analytical Chemistry.

2006,78,7552-7562

2011

Field Amplified Separation in Capillary Electrophoresis : A Capillary Electrophoresis Mode

In capillary electrophoresis (CE), efficiency, resolution, and analysis speed are directly related to the local electric field. A well known approach to locally increase the electric field is to use field-amplified capillary electrophoresis (FACE). In FACE, the capillary is filled with two buffer solutions usually at the same pH and different concentrations. The electric field strength in the region of lower concentration will be amplified, whereas the electric field at the region of higher concentration will be lowered. In this experiment, the capillary was coated using a polymer physically adsorbed. CE experiments were performed using a P/ACE 2050 instrument with UV detection at 214nm. Electrophorethic peak analysis was carried using a commercial peak fitting software. Classical capillary electrophoresis(CE) and field-amplified capillary electrophoresis (FACE) were carried out. A relatively high efficiency and short migration time are obtained using classical CE. Using FACE, an increase in efficiency and resolution and decrease in migration time. In this experiment, using FACE with a concentration factor of 10 between the lowconcentration and high concentration zones, the migration time has been decreased by more than 20% , the efficiency improved by more than 40%, and the resolution improved by more than 5%. FACE shown to provide significant improvement in efficiency and sensitivity and one of the fastest separation using electromigration and standard capillaries.