PAR EG ——-BE (BAB 1S DY 1. (A ) Please choose the correct peptide according to the structure. (A) Leu-Ser-Tyr-Cys-Thr (B) Val-Ser-Tyr-Cys-Thr (C) Leu-Ser-Phe-Cys-Thr (D) Val-Ser-Phe-Cys-Thr oH HOH buy Hg OHO yet Mk Ok or we PRO Ty 8 ¢ we § oH & 2. (0 ) Please give the net charge at pH 1.0 for above peptide.(A) -2 (B) -1 (C) 0D) +1 3.( (g) Which is incorrect about antibody molecule? (A) antibody is made fom two heavy chains and two light chains (B) All chains are held together by hydrogen bonds (C) Cu domains are the same in all antibodies (D) Cx domains are the same in all antibodies 4.(C_) Please choose an amino aeid according to the tration curve, (A) Asp (BWVal (Lys (D) Ser iI «te 7 aa we qm pea a Equivalents of OH s(P, ) The chirality of an amino acid results from the fact that its a carbon: (A) is « carboxylic acid. (B) is bonded to four different chemical groups. (C) is symmetric. (D) has no net charge. 6.( (_) Which of the following is not correct concering cooperative binding ofa ligand toa protein? (A) Te is usually associated with proteins with multiple subunits. (B) It is usually a form of allosteric interaction. (C) It rarely occurs in enzymes, (D) It results in a sigmoidal binding curve. 7. C) What is a zwitterion?(A) A molecule that has just a positive charge (B) A molecule that has no charge (© A molecule that contains both a positive and a negative charge (D) A molecule that has just a negative charge 8.((_ ) Which amino acid can stabilize protein structures by forming covalent cross-links between polypeptide chains? (A) Met (B) Ser (C) Cys (D) Gin 9 ) The fundamental cause of sickle-cell disease is a change inthe structure of: (A) red eels. (B) hemoglobin. (C) capillaries. (D) blood. 10¢(__.) The role of an enzyme in an enzyme-catalyzed reaction is to: (A) ensure that the product is more stable than the substrate. (B) make the free-energy change for the reaction more favorable. (C) increase the rate at which substrate is converted into product. (D) ensure that all the substrate is converted to product.‘You perform enzyme kinetics on wild type NDMase and two of the mutants (Asp999 and Gly999) that have been purified for you by a colleague in your laboratory. You obtain the following Michaclis-Menton curves for the three enzymes: ToT eda (WT: wild type) Oe ‘Compare the kinetic parameters (Km and Vim) of the Asp999 and Gly999 enzymes to those of the wild type NDMase. Ifa parameter has changed, be sure to say how it has changed (or). If ‘a parameter is the same as wild type, indicate this as well. (A) increased (B) decreased (C) no change Km Vinax. ‘Asp999 anc A) ace ) Gly999 a3yc_ Cc) aC B) ‘Which part(s) of the enzymatic reaction (generalized as E + $—ES— E + P) is affected 15.(\ dim the Asp999 mutant?(A) E + SES (B) ESE +P (C) ES S+E 16( g) Inthe Giy999 mutant? (A) E+ S~ES (B)ES+E+P(C)ESS+E Give the enzyme reaction name for the enzyme that would catalyze each of the following reactions. R49 a Hg Hj¢-C-C-OH ——* H,C-CH-C-OH b TRIACYLGLYCEROL ~—— DIACYLGLYCEROL+FATTY ACIE fon gt OH He, gH OH GTS: CH,—-CH-CH, CH,—CH-CH, (A) Ligase (B) Lyase (C) oxidoreductase (D) Hydrolase (E) Transferase (F) Isomerase 17 C) Enzyme a 18.( 2) ) Enzyme b 19. YEnzyme ¢ 20.( (_ )How is trypsinogen converted to trypsin?(A) Two inactive trypsinogen dimers pair to form an active ‘rypsin tetramer. (B) A protein kinase-catalyzed phosphorylation converts trypsinogen to trypsin. (C) Proteolysis of trypsinogen forms trypsin.(D) Trypsinogen dimers bind an allosteric modulator, cAMP, ~causing dissociation into active trypsin monomers, 21.( __ )Which of the following monosaccharides is not an aldose? (A) ribose(B) glucose (C)fructose (D)glyceraldehydes 22. B ) ____ breaks down to glucose and fructose when it is added to a beverage containing water. (A) Lactose (B) Sucrose (C) Starch (D) Maltose 23.( A, )Which of the following statements is false? (A) Glu residues are particularly abundant in collagen (B) Collagen is a protein in which the polypeptides are mainly in the triple-helix conformation (© o-keratin is a protein in which the polypeptides are mainly in the a-helix conformation (D) Disulfide linkages are important for keratin structure 24.( B, )Which of following is an epimer pair? (A) D-glucose and D-suerose (B) D-glucose and D-galactose (©) D-glucose and L-glucose (D) a-D-glucose and f-D-glucose 25. V )in glycoproteins, the carbohydrate moiety is always attached through the amino acid residues: (A) glycine, alanine, or aspartate (B) glutamine or arginine (C) aspartate or threonine (D) asparagine, or threonine 26. B, yPlease choose the structure of fD-Glucofsrnose: gH,0H 004 ot CH,OH Wo oe ; (A) (B) om «) OH Ni @) Hoh sid OH s