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US Signal 2 MHz, 6 V, 150 seconds (DMEM/F-12 with 10% FBS)

1 day Plate # 1 (8x/day) Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12 Plate # 2 (8x/day) Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12 Plate # 3 (8x/day) Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12 Plate # 4 (8x/day) - 1 day Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12

3 days Plate # 6 (4x/day) Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12 Plate # 7 (4x/day) Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12 Plate # 8 (4x/day) Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12 Plate # 9 (4x/day) - 3 days Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12

3 days Plate # 11 (8x/day) Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12 Plate # 12 (8x/day) Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12 Plate # 13 (8x/day) Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12 Plate # 14 (4x/day) - 3 days Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12

5 MHz, 2.5 V, 60 seconds (DMEM/F-12 with 10% FBS)

8 MHz, 9.1 V, 37.5 seconds (DMEM/F-12 with 10% FBS)

Postive Control 5 MHz, 10 V, 60 seconds (DMEM/F-12 with 10% FBS)

Media Control 5 MHz, 10 V, 60 seconds (RPMI 1640 with 10% FBS)

Plate # 5 (8x/day) - 1 day Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12

Plate # 10 (4x/day) - 3 days Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12

Plate # 15 (4x/day) - 3 days Well # 1 Well # 4 Well # 7 Well # 10 Well # 2 Well # 5 Well # 8 Well # 11 Well # 3 Well # 6 Well # 9 Well # 12

Well # 1, 2, 3 F- Actin, Nucleus, Nuclear Actin Well # 4, 5, 6 F-Actin, Tubulin and Vimentin Well # 7, 8, 9 F-Actin, Paxallin and Vinculin Well # 10, 11, 12 For Control (No US )Purpose

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