EFFECT OF DRUGS ON ISOLATED RATS HEART (SIMULATION) LEARNING OBJECTIVES At the end of the class, the student should

be able to: 1. Identify the effect of different drugs on the rhythm and amplitude of contractions in isolated rats heart. 2. Identify unknown drugs by observing their effect on different receptors present in the rats heart. STUDENT ACTIVITY 1. Do the simulated experiment on the computer. 2. Draw the various dose response curves showing effect of various drugs on isolated rats heart.

Notes I took Iso causes increase Receptor anagonists H2 anatagonists If nothing happens it is a B1 agonist Properpanol beta blocker For H2 histomine, and symatimine B1, and H2 Which drugs stimulating the heart Agonist addrenolygn Antagonist.. atenolol Inhibitory drugs Addrenoly non-specific Histamine..cimetidine Digitalis is direct acting stimulant Any drug that will stimulate Nm or M2 will inhibit the heart H2 is histamine Nicotine small dose at Nm Acetocholen is at M2 Trimethaphan / mecamylamine, Atrophine the blocker for acetylocholine

Acetycholen acts on M (decrease in HR to use an antagonist , block N , and if there is no effect then it is acting through M) N Open Ract vcs (objectives) Inotropic increase heart rate REFERENCE Basic and clinical pharmacology, 11th edition, Katzung B.G. Available at www.accessmedicine.com.