LnZ?

ML lnPl8l1lCn
8LSLn1Lu 8?
CLL1LSP 8PACAu8
1
S1
1L8M
lnPl8l1C8S
lnhlblLors are chemlcals LhaL reduce Lhe raLe
of enzymlc reacLlons
1he are usually speclflc and Lhey work aL low
concenLraLlons
1hey block Lhe enzyme buL Lhey do noL usually
desLroy lL
Many drugs and polsons are lnhlblLors of
enzymes ln Lhe nervous sysLem
LllLC1 Cl LnZ?ML lnPl8l1lCn
Irrevers|b|e |nh|b|tors Comblne wlLh Lhe
funcLlonal groups of Lhe amlno aclds ln Lhe
acLlve slLe lrreverslbly
Lxamp|es nerve gases and pesLlcldes
conLalnlng organophosphorus comblne wlLh
serlne resldues ln Lhe enzyme aceLylchollne
esLerase
LllLC1 Cl LnZ?ML lnPl8l1lCn
evers|b|e |nh|b|tors 1hese can be washed
ouL of Lhe soluLlon of enzyme by dlalysls
1here are Lwo caLegorles
CompeLlLlve lnhlblLlon
non compeLlLlve lnhlblLlon
LllLC1 Cl LnZ?ML lnPl8l1lCn
ompet|t|ve 1hese compeLe wlLh Lhe
subsLraLe molecules for Lhe acLlve slLe
1he lnhlblLor's acLlon ls proporLlonal Lo lLs
concenLraLlon
8esembles Lhe subsLraLe's sLrucLure closely
LllLC1 Cl LnZ?ML lnPl8l1lCn
-oncompet|t|ve 1hese are noL lnfluenced by
Lhe concenLraLlon of Lhe subsLraLe lL lnhlblLs by
blndlng lrreverslbly Lo Lhe enzyme buL not at the
act|ve s|te
Lxamp|es
Cyanlde comblnes wlLh Lhe lron ln Lhe enzymes
cyLochrome oxldase
Peavy meLals or comblne wlLh
groups
1hese can be removed by uslng a chelaLlng agenL
such as Lu1A
ALlCA1lCn Cl lnPl8l1lC8S
-eat|ve feedback end polnL or end producL
lnhlblLlon
9o|sons snake blLe planL alkalolds and nerve
gases
,ed|c|ne anLlbloLlcs sulphonamldes
sedaLlves and sLlmulanLs
@he sw|tch ||oster|c |nh|b|t|on
AllosLerlc means "other s|te"

ct|ve s|te
||oster|c
s|te
w|tch|n off
1hese enzymes
have two receptor
s|tes
Cne slLe flLs Lhe
subsLraLe llke oLher
enzymes
1he oLher slLe flLs
an lnhlblLor
molecule
lnhlblLor flLs lnLo
a||oster|c s|te
SubsLraLe
cannoL flL
lnLo Lhe
act|ve s|te
lnhlblLor
molecule
@he a||oster|c s|te the enzyme "onoff"
sw|tch

ct|ve
s|te
||oster|c
s|te
empLy
SubsLraLe
flLs lnLo
Lhe act|ve
s|te
1he lnhlblLor
molecule ls
absent
onformat|ona|
chane
lnhlblLor flLs
lnLo a||oster|c
s|te
SubsLraLe
cannoL flL
lnLo Lhe
act|ve s|te
lnhlblLor
molecule
ls present

chane |n shape
Jhen Lhe lnhlblLor ls presenL lL flLs lnLo lLs slLe
and Lhere ls a conformat|ona| chane ln Lhe
enzyme molecule
1he enzyme's molecular shape changes
1he act|ve s|te of Lhe subsLraLe changes
1he subsLraLe cannoL blnd wlLh Lhe subsLraLe
2008 Paul Billiet ODWS
-eat|ve feedback |s ach|eved
1he reacLlon slows down
1hls ls not compeLlLlve lnhlblLlon buL lL ls
reverslble
Jhen Lhe lnhlblLor concenLraLlon dlmlnlshes
Lhe enzyme's conformaLlon changes back Lo
lLs acLlve form
2008 Paul Billiet ODWS
llg negaLlve feedback lnhlblLlon
1PAnk ?Cu