GENE TRANSFER TECHNOLOGIES, STRAIN IMPROVEMENT

INTRODUCTION

Gene transfer technology is to introduce gene or DNA in living organisms.

Gene Transfer Technologies

Physical Transfection

Chemical Transfection

Transduction

Bactofection

Physical Transfection Method:

Naked DNA is directly transferred into the cell by explioting a physical force. 1. 2. 3. 4. Microinjection Ultrasound Electrporation Particle Bomcardment

Chemical Transfection Method:

cells are persuaded to take up the DNA from their surroundings where, it involves formation of complex that facilitates interactions with the plasma membraneof the cell. 1. Calcium Phosphate method 2. Many other chemicals are used.

Transduction:
Delivery using a Viral vector is called TRANSDUCTION. The exogenetic material is added to the complete genome or a part of the genome.

Bactofection:
Delivery using a Bacterial Cell is called as BACTOFECTION. Here transgene is delivered on a plasmidwhich is carreid by the bacterium