United States Patent 19 Lo CANINA AT 'US005242820A. (11) Patent Number: 5,242,820 [45] Date of Patent: Sep. 7, 1993 [54] PATHOGENIC MYCOPLASMA [75] Inventor: Shyh-Ching Lo, Potomac, Md. American Registry of Pathology, Washington, D.C. [21] Appl. No: 710,361 [22] Filed: Jum. 6, 1991 [73] Assignee: Related U.S. Application Data [63] Continuation-in-part of Ser. No. 265920, Nov. 2 1988, abandoned, which is 2 continuation-in-partof Ser. No. 875,535, Jun. 18, 1986, abandoned, [51] Int. cus CAN 5/00; C12N 5/02; C12N 1/00; C12Q 1/70 [9] Usa. 435/240.2; 435/5; 435/872 [58] Field of Seareh 435/810, 5, 872, 240.2 sq References Cited PUBLICATIONS Marquart etal (1985) Mycoplasma-Like Structures Eur J Clin Microbiol 4(1):73-7. Loetal (1989) A Novel Virus-like Infectious Agent ‘Am J Trop Med Hyg 40(2):213-226. Loet al (1989) Identification of M Incognitus... Am. J. ‘Trop-Med. Hyg 41(5):601-616. Lo et al (1989) Association of the Virus-like Agent... ‘Am J Trop Med Hyg 41(3):364-376. Lo et al (1989) Fatal Infection of Silvered Leaf Mon- Keys... Am. T Trop Med Hyg 40(4):399-409. Lo ef al (1989) Virus-like Infectious Agent ‘Trop Med Hyg 41(5):586-600. Marquart et al (Feb. 1985) Abstract Only Eur J Clin Microbiol 4(1):73-74. Hu et al (1990) Gene 93:67-72. Primary Examiner—Christine M. Nucker ‘Assistant Examiner—D. R. Preston ‘Attorney, Agent, or Firm—Venable, Bactjes, Howard & Civiletti (57) ABSTRACT ‘The invention relates to a novel pathogenic myco- plasma isolated from patients with Acquired Immune Deficiency Syndrome (AIDS) and its use in detecting antibodies in sera of AIDS patients, patients with AIDS-related complex (ARC) or patients dying of dis- eases and symptoms resembling AIDS diseases. The invention further relates 10 specific DNA sequences, antibodies against the pathogenic mycoplasma, and their use in detecting DNA or antigens of the patho- genic mycoplasma or other genetically and serologi- cally closely related mycoplasmas in infected tissue of patients with AIDS or ARC or patients dying of symp- toms resembling AIDS diseases. The invention still further relates to a variety of different forms of vaccine against mycoplasma infection in humans and/or ani- mals. AmJ 2 Claims, 39 Drawing SheetsUS. Patent Sep. 7, 1993 Sheet 1 of 39 5,242,820USS. Patent Sep. 7, 1993 Sheet 2 of 39 5,242,820 FIG. 1D