RESTICTION

ENDNONUCL
- EASES
Product Source


8omnl


8q1ll


lvol

cokl
5` sticky
ends
An E. coli strain
that carries the
cloned EcoRI gene
Irom E. coli RY13

nL 8uffer
LCC8l + 8SA aL
37rC

nL 8uffer
LCC8l aL 37rC

nL 8uffer
Lco8l + 8SA
aL 37rc

8omnl
5` sticky
ends
A E. coli strain
that carries the
BamHI gene Irom
Bacillus
amyloliqueIaciens

nL 8uffer 3 +
8SA aL 37rC

nL 8uffer 3 +
8SA aL 37rC

8q1ll
5` sticky
ends
A E. coli strain
that carries the
BglII gene Irom
Bacillus globigii
See row 8am
Pl

nL 8uffer 3 +
8SA aL 37rC

lvol
3` sticky
ends
An E. coli strain
that carries the
cloned PvuI gene
Irom Proteus
vulgari
See row 8am
Pl
See row 8g1ll

lvoll
Blunt ends A E. coli strain
that carries the
PvuII gene Irom
Proteus vulgaris
See row 8am
Pl
See row 8g1ll See row vul

Mbol
5` sticky
ends
A E. coli strain
that carries the
MboI gene Irom
Moraxella bovis
See row 8am
Pl
See row 8g1ll See row vul

nloJlll
5` sticky
ends
A E. coli strain
that carries the
HindIII gene Irom
Haemophilus
inIluenzae
See row 8am
Pl
See row 8g1ll See row vul

nlofl
5` sticky
ends
A E. coli strain
that carries the
HinII gene Irom
Haemophilus
inIluenzae
See row 8am
Pl
See row 8g1ll See row vul

5ooJAl
5` sticky
ends
An E. coli strain
that carries the
cloned Sau3Al
See row 8am
Pl
See row 8g1ll See row vul
gene Irom
Staphylococcus
aureus 3A

Alol
Blunt ends An E. coli strain
that carries the
cloned AluI gene
Irom Arthrobacter
luteus
See row 8am
Pl
See row 8g1ll See row vul

1opl
5` sticky
ends
An E. coli strain
that carries a Taq
u
I
overproducing
plasmid
See row 8am
Pl
See row 8g1ll See row vul

noelll
5` sticky
ends
A E. coli strain
that carries the
HaeIII gene Irom
Haemophilus
aegypticus
See row 8am
Pl
See row 8g1ll See row vul

Notl

See row 8am
Pl
See row 8g1ll See row vul
















lvoll

Mbol

nloJlll

cokl
nL 8uffer 2 aL 37rC
Lco8l may show sLar
acLlvlLy ln Lhls buffer
nL 8uffer 4 aL 37rC
Lco8l may show sLar
acLlvlLy ln Lhls buffer
uouble dlgesL ls noL
recommended A
sequenLlal dlgesL ls
requlred uslng each
enzyme ln lLs supplled nL
8uffer
LCC8l nebuffer Lco8l
Plndlll nL 8uffer 2

8omnl
nL 8uffer3 + 8SA aL 37rC
or nL 8uffer 2 + 8SA aL
37rc 8amPl may exlhlglL
sLar acLlvlLy ln Lhls buffer
nL 8uffer3 + 8SA aL 37rC
or nL 8uffer 4 + 8SA aL
37rc 8amPl may exlhlglL
sLar acLlvlLy ln Lhls buffer
uouble dlgesL ls noL
recommended A
sequenLlal dlgesL ls
requlred uslng each
enzyme ln lLs supplled nL
8uffer
8amPl nL 8uffer 3
Plnd lll nL 8uffer 2

8q1ll
nL 8uffer3 aL 37rC nL 8uffer3 aL 37rC *3

lvol
nL 8uffer3 + 8SA aL 37rC nL 8uffer3 + 8SA aL 37rC *6

lvoll
nL 8uffer2 aL 37rC or nL
8uffer 4 aL 37rc
nL 8uffer2 aL 37rC

Mbol
See row vull nL 8uffer2 aL 37rC

nloJlll
See row vull See row Mbol

nlofl
See row vull See row Mbol See row Plndlll

5ooJAl
See row vull See row Mbol See row Plndlll

Alol
See row vull See row Mbol See row Plndlll

1opl
See row vull See row Mbol See row Plndlll

noelll
See row vull See row Mbol See row Plndlll

Notl
See row vull See row Mbol See row Plndlll


nlofl

5ooJAl

Alol

cokl
nL 8uffer 4 aL 37rC
Lco8l may show sLar
acLlvlLy ln Lhls buffer
nL 8uffer 1 + 8SA aL
37rC Lco8l may show
sLar acLlvlLy ln Lhls
buffer
nL 8uffer 4 aL 37rC
Lco8l may show sLar
acLlvlLy ln Lhls buffer

8omnl
nL 8uffer 4 + 8SA aL
37rc 8amPl may show
sLar acLlvlLy ln Lhls
buffer
*1 nL 8uffer 4 + 8SA aL
37rC 8amPl may show
sLar acLlvlLy ln Lhls
buffer

8q1ll
*4 *3 *4

lvol
*6 nL 8uffer 2 + 8SA aL
37rC
*6

lvoll
nL 8uffer2 aL 37rC or
nL 8uffer 4 aL 37rc
nL 8uffer 1+ 8SA aL
37rC
nL 8uffer2 aL 37rC or
nL 8uffer 4 aL 37rc

Mbol
nL 8uffer 4 aL 37rc nL 8uffer 4 + 8SA aL
37rC
nL 8uffer 4 aL 37rC

nloJlll
nL 8uffer2 aL 37rC nL 8uffer1+8SA aL 37rC
or nL 8uffer2 + 8SA aL
37rC
nL 8uffer2 aL 37rC

nlofl
nL 8uffer 4 + 8SA aL
37rC
nL 8uffer 4 aL 37rC

5ooJAl
See row Plnfl nL 8uffer 1 + 8SA aL
37rC or nL 8uffer 4 +
8SA aL 37rC

Alol
See row Plnfl See row Sau3Al

1opl
See row Plnfl See row Sau3Al See row Alul

noelll
See row Plnfl See row Sau3Al See row Alul

Notl
See row Plnfl See row Sau3Al See row Alul



1opl

noelll

Notl

cokl
nL 8uffer 4 + 8SA aL
37rC wlLh Lco8l Lhen
add 1aql and ralse
LemperaLure Lo 63rC
Lco8l may show sLar
acLlvlLy ln Lhls buffer
nL 8uffer 4 aL 37rC
Lco8l may show sLar
acLlvlLy ln Lhls buffer
nL 8uffer Lco8l + 8SA
aL 37rC

8omnl
*2 nL 8uffer 4 + 8SA aL
37rc 8amPl may show
sLar acLlvlLy ln Lhls buffer
nL 8uffer 3 + 8SA aL
37rC


8q1ll
*3 *3 nL 8uffer3+ 8SA aL
37rC

lvol
*7 nL 8uffer 2 + 8SA aL
37rC
nL 8uffer3+ 8SA aL
37rC

lvoll
*8 nL 8uffer2 aL 37rC or nL
8uffer 4 aL 37rc
nL 8uffer3+ 8SA aL
37rC

Mbol
*8 Mbol lnsLead of vull nL 8uffer 4 aL 37rC nL 8uffer3+ 8SA aL
37rC

nloJlll
*9 nL 8uffer2 aL 37rC *3+8SA

nlofl
*8 Plnfl lnsLead of vull nL 8uffer 4 aL 37rC *6

5ooJAl
*8 Sau3Al lnsLead of
vull
nL 8uffer 4 + 8SA aL
37rC
*3 + 8SA AcLlvlLy for
boLh are low (30)
SequenLlal dlgesL
suggesLed

Alol
*8 Alu l lnsLead of vull nL 8uffer 4 + 8SA aL
37rC
*6

1opl
*8 Paelll lnsLead of vull *6

noelll
See row 1aql *3 + 8SA

Notl
See row 1aql See Paelll
*1 ulgesL ln nL 8uffer 1 + 8SA aL 37rC AL leasL one enzyme has 100 acLlvlLy ln Lhls buffer so
addlLlonal unlLs of enzyme and/or longer lncubaLlon Llme may be necessary 8amPl may show sLar
acLlvlLy ln Lhls buffer C8 ulgesL ln nL 8uffer 4 + 8SA aL 37rC 8amPl may show sLar acLlvlLy ln Lhls
buffer
*2 ulgesL ln nL 8uffer 3 + 8SA aL 37rC wlLh 8AmPl Lhen add 1aql and ralse LemperaLure Lo 63rC
C8 ulgesL ln nL 8uffer 4 + 8SA aL 37rC Lhen add 1aq l and ralse LemperaLure Lo 63rC 8amPl may
show sLar acLlvlLy ln Lhls buffer
*3 ulgesL ln nL 8uffer 2 aL 37rC AL leasL one enzyme has 100 acLlvlLy ln Lhls buffer so
addlLlonal unlLs of enzyme and / or longer lncubaLlon Llme may be necessary
*4 ulgesL ln nL buffer 3 aL 37rC AL leasL one enzyme has 100 acLlvlLy ln Lhls buffer so
addlLlonal unlLs of enzyme and/or longer lncubaLlon Llme may be necessary
*3 ulgesL ln nL 8uffer 3 + 8SA aL 37rC wlLh 8g1 ll Lhen add 1aql and ralse LemperaLure Lo 63rC
*6 ulgesL ln nL 8uffer 3 + 8SA aL 37rC AL leasL one enzyme has 100 acLlvlLy ln Lhls buffer so
addlLlonal unlLs of enzyme and/or longer lncubaLlon Llme may be necessary
*7 ulgesL ln nL buffer 3 + 8SA aL 37rC wlLh vul add 1aql and ralse LemperaLure Lo 63rC
*8 ulgesL ln nL 8uffer 4 +8SA aL 37rC wlLh vull Lhen add 1aql and ralse LemperaLure Lo 63rC
*9 ulgesL ln nL 8uffer 2 + 8SA wlLh hlnd lll Lhen add 1aql and ralse LemperaLure Lo 63rC AL leasL
one enzyme has 100 acLlvlLy ln Lhls buffer So addlLlonal unlLs of enzyme and/or longer
lncubaLlon Llme may be necessary







lSCSCPlZCML8S





#ecognition Site
Isoschizomers
(if any)
.4#I
%%
-
,2I
%
-
II
%%
-
!;:I
%
pv&I, MvrI, Ple19I
!;:II
%
-
-4 I
%
1uI, sp143I,ssMI, stKTI`,
DpnII, K:o9I, NdeII, Sau3AI
3/III
%%
-
31I
%
-
$,:I
%
1uI, sp143I, stKTI`, stMBI,
DpnII, K:o9I, MboI, NdeII
:I
%
luBI
%,6I
%
-
,0III

shI, snI, spANI, suRI, PhoI