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4 :
(Lipton Linea tea) HPLC
:
A : 110125
: 18/11/2011
: 25/11/2011
HPLC
1.)
/ (HPLC High Performance / Pressure
Liquid Chromatography).
/
.
2.)
2.1 GC HPLC
2 ( ) 2
..
(stationary) (mobile) , .
, .
. (
o Tswett .)
GC .
LC
- .
- PLC .
PLC GC
- A
- , ( )
, .
- , .
2.2 HPLC
.1 HPLC.
. 1
1)
2)
3)
4)
5)
6)
6)
7)
8) ( )
9) ()
10)
11) (PC,,)
12)
2.3 HPLC
- 500 ml
-
.
2 HPLC :
.
a) I (1 - Solvent) 1 .
14 6000 psi ( 400 bar) .
T ( 2004) : UPLC (Ultra Performance liquid Chromatography)
15000 psi ( 1000 bar).
T
( ).
( )
.2
. 1-500 L
(.2). 2
.
.3 PLC
2-3 mm.
10 100 cm
GC.
(., / )
HPLC (, , .)
( ..).
/
.
- A
(.4).
.
(190-600 nm) .M
. Beer- Lambert
.
:
a)A (RI refraction index)
b)
c) ,FID,..
.4 HPLC
3
2.4 HPLC
HPLC (,,).O
(,,)
. ,
.
( )
.
HPLC
X ,
( , )
2 :
a) , ()
( ) , ..
b) X , ( ,
, , ..)
(, .) . : X (
), , ( ,
), (
)
a) ( ) tRX
( ) tRS .
, ( 1
) , 2
(
)
b) H :
- ()
- ,
, ( ) .
.
,
( ) .
3.)
,,,,,,,,,,
, , , , , ,
Lipton Linea Tea HPLC.
3.1
15 min ( )
.
( 0,45 nm) .
CH3OH 34% H20 64%.
3.2
Hewlett- Packard 1100 HPLC, Macherey Nagel
Nucleosil 100 S C18 240 * 4,6 mm.O
274 nm. H 12 min
(t = 0 )
,
4
200 -700 nm ( )
100 l 20 l.
4.)
.6 1.
1.
,
.7
2:
,
6
,
:
0,16255 mg/ml.
1) Skoog,Holler,Nieman (2005) ,
2) http://www.waters.com/waters/nav.htm?cid=10048919&locale=en
3.). . (2008) ,
,
4)Biosciences Sheffield, www.schu.ac.uk