SINH TNG HP PROTEIN

GIAI ON KHI U PHN BIT T BO NHN THT V T BO NHN NGUYN THY

Ging vin: Ts. Nguyn T Anh B mn Vi sinh
i hc Dc Tp. H Ch Minh Nhm sinh vin thc hin: * Lng Minh Tun * L Th Ngc Mai

* Nguyn Phi Hip 1

* inh Thy Hng Tin

* Th Thanh Hon

* Nguyn L Hi Trn * Trn M Thin Thanh

GIAI ON KHI U DCH M RIBOSOM

Giai on khi u, c s tham gia ca 2 tiu n v ca Ribosom, mt phn t mARN ( sp thng hng vi Ribosom) v tARN khi u mang methionin hay formyl-methionin t bo nhn nguyn thy Cc yu t khi u Vi khun: IF1, IF2, IF3 Cc yu t khi u nhn tht: eIF1, eIF2, eIF3, eIF4A, eIF4B, eIF4E,eIF4F,eIF5,eIF6,GEF

S KHI U CHUI PEPTID T BO PROKARYOTE

GIAI ON KHI U PROKARYOTE

-

1.
-

2.

c trng cho s khi u chui peptid vi khun l s tng tc trc tip gia mARN v rARN ca tiu n v nh 30S IF1 gip tiu n v gn vo mARN v ngn cn cc tARN gn vo vng thuc v tr A trn tiu n v. Gn IF3 vo 30S, ngn cn s ti hnh thnh ribosome hon chnh. IF2 lm trung gian trong vic ghp tARN khi u vo tiu n v 30S. IF2 to phc 3 yu t vi GTP v tARN khi u: IF2-GTP-fMet-tARN-fMet. Lin kt gia trnh t Shine-Dalgarno vi trnh t b sung ca 16S rARN c hot ha bi IF3. Khi Codon khi u v fMet-tARNiMet bt cp vi nhau th tiu n v nh thay i hnh dng lm gii phng IF3.

GIAI ON KHI U PROKARYOTE

-

3.
-

tARN u tin c mang N-Formyl-methionine n gn trc tip vo tiu n v nh (tARN khi u gn trc tip vi v tr P (khng qua v tr A), tARN c anticodon c th bt cp vi AUG hay UGU. tARN khi u ny gi l fMettARNifMet S vng mt ca IF3 cho php tiu n v ln 50s gn vo tiu n v nh 30S. Nh c tiu n v ln gn vo, hot tnh GTPase ca IF2GTP c kch thch c th thy phn GTP IF2-GTP to thnh c i lc thp i vi ribosome v tARN khi u dn n s gii phng IF2-GTP v IF1. Nh vy, phc hp khi u cui cng c to thnh bao gm ribosome 70S c gn ti codon khi u ca mARN vi fMet-tARNifMet ti v tr P cn v tr A trng. Phc hp ny sn sng tip nhn 1 tARN mang amino acid vo v tr A bt u tng hp chui polypeptid.

GIAI ON KHI U PROKARYOTE

S MIU T S KHI U T BO PROKARYOTE

S KHI U CHUI PEPTID T BO EUKARYOTE

7

GIAI ON KHI U EUKARYOTE

-

Ribosome ca t bo Eukaryote tch ri ra thnh 2 tiu n v: tiu n v ln 60s v tiu n v nh 40s eIF6 tng tc vi tiu n v ln 60S to phc eIF6-60S. eIF3 v eIF4E kt hp vi tiu n v nh 40S to phc 43S. Phc 43S gn vo mARN chp 5 hnh thnh nn phc hp chp cc yu t khi u 43s-eIF2-GTP-Met-tARNiMeteIF3 Tiu n v 43S qut dc theo mARN tm AUG khi u. Qu trnh ny cn ATP v c th tho xon nt kp tc. Codon nhn dng bng s bt cp b sung gia anticodon ca tARN khi u v codon khi u. S bt cp ny thc y phng thch eIF2 v eIF3. Cho php tiu n v 60S gn vo tiu n v nh. S gn ny dn n phng thch cc yu t khi u thng qua s thy phn GTP di tc dng ca eIF5. Cui cng Met-tARNiMet c a vo v tr P ca phc khi u 80S. Lc ny Ribosome trong t th sn sng tip nhn aminoacyl-tARN vo v tr A.

GIAI ON KHI U EUKARYOTE

S MIU T S KHI U T BO EUKARYOTE

PHN BIT GIAI ON KHI U T BO NHN THT V T BO NHN NGUYN THY

TB. Vi Khun TB. Nhn Tht

Codon khi u tARN khi u tARN mang

AUG( hay GUG) tARNfMet Formyl Methionin

AUG tARNiMet Methionin

vi khun ngoi codon khi u AUG cn c GUG mt s mARN tARNfMet khng ch vn chuyn Methionin cn vn chuyn Formyl-methionin

10

PHN BIT GIAI ON KHI U T BO NHN THT V T BO NHN NGUYN THY

Yu t TB. Vi khun Yu t TB. Nhn tht Chc nng

IF-1
IF-2 IF-3

eIF -1
eIF -2 eIF - 3 eIF- 4A eIF- 4B eIF- 4E,- 4F

Vai tr cha r
Gn Met-tARN (hay fMet-tARN) vo ribosom thnh phc hp vi GTP Ngn cn s kt ni ca cc tiu n v ribosom Nhm yu t lin quan p ng cho vic gn chp 5 vo u mARN v tho xon cu trc bc 2 to iu kin cho ribosom u vo condon khi u

eIF - 5
eIF - 6 GEF

Gii phng eIF2,eIF3 khi ribosom, gip cho tiu n v 60S kt ni

Tham gia vo vic tch ribosom thnh cc tiu n v Yu t bin i nucleotid Guanin, ti sinh eIF-2 bi s bin i trung gian GDP thnh GTP, trong qu trnh tng t ti sinh EF-Tu bi EF-Ts

11

PHN BIT GIAI ON KHI U T BO NHN THT V T BO NHN NGUYN THY

TB. Vi khun TB. Nhn tht

Trnh t dn mARN

Theo trnh t ShineDalgarno C trnh t CCUCC

Theo m hnh ca Kozak Khng c trnh t CCUCC Monocistron Khi u gn vi chp 5 mARN

mARN

Polycistron

S khi u dch Khng nht thit m u 5 mARN

12

13