Pre-PhD Syllabus Draft

Unit I: CHROMOSOME STRUCTURE AND CHROMATIN ORGANIZATION 1.1 1.2 1.3 Prokaryotic chromosome structure Molecular structure of eukaryotic chromosome Centromere and Telomere, Specialized chromosomes. Polytene and Lampbrush.

1.4 Components of Chromatin Nucleic acids, histones and non-histones, 1.5 Organization of Nucleosomes, core particle location of H1, organization of nucleosomes in chromatin, Solenodis, loops and scaffolds, active and inactive chromatin Unit-II: GENOME ORGANIZATION 2.1 2.2 2.3 Nuclear genome: General features of Human Genome Unique sequences and families of reiterated sequences Extragenic repeated sequences and transposable elements. Transposition via-mRNA Intermediate, DNA Transposons 2.4 Gene families-Clustered and Dispersive Eg: histone gene clusters, haemoglobin gene clusters 2.5 2.6 2.7 Pseudogenes. Mitochondrial genome organization Chloroplast genome organisation

UNIT III: GENE EXPRESSION 3.1 3.2 3.3

& REGULATION

Mechanism of transcription and regulation of gene expression in prokaryotes Mechanism of transcription of protein coding genes in eukaryotes Transcriptional regulation of eukaryotic gene expression

3.4

Post transcriptional modification & Post transcriptional gene silencing SiRNA, Small RNA, Micro RNA

3. 5

Transcription and processing of non coding RNA

3.6

Genetic code Characteristics, universal and alternate genetic codes, codon usage and bias.

3.7 Components of translational machinery Ribosomes, tRNA, aminoacyl tRNA synthetases and translation factors 3.8 Process of translation initiation, elongation and termination

Unit IV: GENETIC ENGINEERING 4.1 4.2 4.3 4.4 Restriction enzymes and cloning vectors Construction of genomic and c DNA libraries Selection of recombinant Clones Applications of r DNA technology