UNIVERSITY OF MINNESOTA

INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE (IACUC)

Appendix A Alternatives to Animals - Classified in Categories B or C

Federal regulations and University Policy require consideration of the use of alternatives to procedures that may cause more than momentary or slight pain or distress to animals. Complete this appendix for each of the species that will be used in this ACUP. SPECIES: 1. Briefly describe how you have considered each of the following alternatives or how they are not applicable:
Replacement of vertebrate animals (e.g. with in vitro models, computer models or less sentient animals)

Refinement of experimental procedures to minimize pain or distress (e.g. early endpoints; use of analgesics, anesthetics, or sedatives; techniques that reduce stress in the animals):

Reduction in the number of animals (e.g., using appropriate statistical methods in the design and analysis of the study; reduction in experimental variability by using animals of defined genetic or microbiological status; sharing tissue among investigators):

2. Methods used to search for alternatives (indicate all that apply): a. Literature search conducted NOTE: at least one database must be used.
(See http://www.ahc.umn.edu/rar/ethics.html#Alternatives or http://www.nal.usda.gov/awic for resources to assist in the search) List names of databases:

1. 2. 3.
Keywords used in database search (specific to animal use, not simply the science of the research). It is highly recommended that one of the keywords used be alternative:

Appendix A Rev 3/11

UNIVERSITY OF MINNESOTA

INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE (IACUC)

Date the search was completed: Years searched (should go back several years):

b. Consultation with colleagues. Please note that this consult should be related to alternatives to the use of animals, not simply the science behind the research.
Name(s) of those with whom you consulted:

Provide their qualifications:

Date of consult: Content of consult:

c. Other information/service utilized. Elaborate, providing specific information:

Appendix A Rev 3/11