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2008 09 M.sc. Bio Chemistry Part II
2008 09 M.sc. Bio Chemistry Part II
w. e. f. 2008 - 09
Course Structure at a Glance Semester III BC-301: Plant Biotechnology BC-302: Molecular Biology BC-303: Environmental Toxicology BC-304: Lab. Course - IV BC-305: Lab. Course V (Project Work / Dissertation) Semester IV BC-401: Microbial Biotechnology BC-402: Genetic Engineering & rDNA Technology BC-403: Biochemistry of Natural Products BC-404: Applied Biochemistry & Animal Tissue Culture BC-405: Lab. Course VI
Semester I and IV will have 04 theory courses and 1 practical course each whereas semester II and III will have 3 theory courses and 2 practical courses each. Practical examination will be held annually. Each course will have a weightage of 100 marks (20 marks internal and 80 university examination) Each theory course to be completed in 50 lectures of 60 min duration each
Recommended Books; 1. Applied and fundamental aspects of plant cell, tissue and organ culture: J. Reinert and Y. P. S. Bajaj. 2. Plant cell biotechnology: R. Endress (Springer-Verlag). 3. Biotechnology in agriculture: M. S. Swaminathan. 4. Secondary metabolism in plant cell culture: P. Morrist, A. H. Scragg, A. Stafford, M. W. Fowles. 5. Agricultural Biotechnology: S. S. Purohit (Agro Botanica). 6. Plant Biotechnology: J. Hammond, P. McGravery and V. Yusibov (Eds.) (Springer-Verlag). 7. Plant cell and tissue culture for the production of food ingredients: T. J. Fu, G. Singh and W. R. Curtis (Eds.) (Kluwer Academic/Plenum Press). 8. Biotechnology in crop improvement: H. S. Chawla (International Book Distributors). 9. Plant Tissue Culture: K. K. De (New central Book Agency). 3
10. Plant cell, tissue and organ culture-fundamental methods: O. L. Gamborg and G. C. Phillips (Eds.) (Narosa Publishing House). 11. A text book of biotechnology: R. C. Dubey (S. Chand & Co. Ltd.). 12. Biotechnology: P. K. Gupta (Rastogi Publications). 13. Plant propagation-principles and practices: H. T. Hartmann and D. E. Kester (Prentice Hall India Pvt. Ltd.). 14. Practical applications of plant molecular biology: R. J. Henry (Chapman & Hall). 15. Biotechnology an introduction: S. R. Barnum (Thomson Brooks/Cole). 16. Methods in plant tissue culture: U. Kumar (Agrobios).
Recommended Books; 1. Genes: B. Lewin (Oxford University Press). 2. Biochemistry: L. Stryer (W.H. Freeman & co.). 3. Lehninger principles of biochemistry: D. L. Nelson & M. M. Cox (Macmillan Worth Publishers). 4. Molecular cell biology: J. Darnell, H. Lodish & D. Baltimore (Scientific American Books). 5. Fundamentals of biochemistry: D. Voet, J. Voet & C. Prati (John Wiley & Sons). 6. Molecular biology of genes: J. Watson, N. H. Hopkin, J. W. Roberts, J. P. Stertz & A. M. Weiner (Freeman Co.) 5
7. The biochemistry of the nucleic acids: R. L. P. Adams, J. T. Knowler & D. P. Leader (Chapman & Hall). 8. Biotechnology an introduction: S. R. Barnum (Thomson Brooks/Cole). 9. Cell and molecular biology: E. D. P. De Robertis, E. M. F. De Robertis, Jr. (Lea & Febiger international edition, Info Med). 10. Principles of genetics: E. J. Gardner, M. J. Simmons, D. P. Snustad (John Wiley & sons Inc.). 11. A history of molecular biology: M. Morange (Oxford University Press). 12. Cell and molecular biology: P. Sheeler, D. E. Blanchi (John Wiley & sons Inc.). 13. Molecular biology and microbes: S. Srivastava and V. Singhal (Anmol publications Pvt. Ltd.). 14. Concepts in molecular biology: S. C. Rastogi, V. N. Sharma & A. Tandon (Wiley Eastern Ltd.). 15. Molecular biology: P. K. Gupta (Rastogi Publications).
Recommended Books; 1. Casarett and Doulls toxicology: C. D. Klaassen, M. O. Amdur, J. Doull (Macmillan Publishing Co.). 2. Hayes principles and method of toxicology: A. Wallace and Hages (Eds.) (Raven Press). 3. Hamilton and Hardys industrial toxicology: R. D. Harbison (Tampa). 4. Basic toxicology fundamental target organs and risk assessment: F. C. Lu. 5. Environmental biology: K. C. Agrawal (Agor Botanica). 7
6. Toxicology: A. Sood (Sarup & sons). 7. Introduction to environmental toxicology impact of chemicals upon ecological systems: W. G. Landis and Ming-Ho Yu (Lewis Publishers Inc.). 8. Hamilton and Hardys Industrial toxicology: R. D. Harbison (Mosby). 9. CRC handbook of toxicology: M. J. Derelanko, M. A. Hollinger (CRC Boca Raton). 10. Environmental toxicology human exposure and their health effects: M. Lipmann (John Wiley & sons Inc.). 11. Principles and methods of toxicology: A. W. Hayes (Raven Press). 12. Toxic and hazardous waste disposal: R. B. Pojasek (Ann Arborscience Publisher). 13. Toxics A to Z a guide to everyday pollution hazards: J. Harte, C. Holdren, R. Schneider and C. Shinley (Bishen Singh Mahendrapal Singh Publishers). 14. Environmental toxicology: M. Satake, Y. Mido, H. Yasuhisa, S. Taguchi, M. S. Sethi, S. A. Iqbal (Discovery Publishing House). 15. Fundamental toxicology for chemists: J. H. Duffus and H. G. J. Worth (Eds.) (The Royal Society of Chemistry). 16. Waste Management: R. K. Sinha and A. K. Sinha (INA Shree Publishers).
Recommended Books; 1. Bitechnology of filamentous fungi: D. B. Franklinstein and C. Ball (BitterworthHeinemann). 2. Biotechnology of antibiotics: Vandamme Drugs and pharmaceuticals, Vol. 22 (Ed.) (Marcel Dekker Inc.). 10
3. Principle of fermentation technology: P. F. Stransberry, A. Whitekar & S. J. Hall ( Aditya Books). 4. Process biotechnology fundamentals: Mukhopadhay (Viva Books Pvt. Ltd.). 5. Microbial technology - fermentation technology, Vol. I & II: A. H. J. Peppler & D. Perlmann (Academic Press). 6. Solid substrate cultivation: H. W. Doelle, D. A. Mitchell & C. E. (Elsevier Applied Science). 7. Industrial microbiology: L. E. Casida JR (New Age International Publishers Ltd.). 8. Industrial microbiology: S. C. Prescott & C. G. Dunn (Agrobios). 9. Biotechnology an introduction: S. R. Barnum (Thomson Brooks / Cole). 10. Basic biotechnology: C. Ratledge and B. Kristiansen (Cambridge University Press).
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Cloning: Steps involved, strategies for each step with details of cDNA synthesis of generation of DNA, joining cDNA to vector DNA by homopolymer tailing , transformation with rDNA, transfection with r-phage DNA, screening by immunochemical and nucleic acid hybridization method. DNA library: cDNA and genomic DNA library. Mapping: S1 nuclease mapping, restriction mapping and their applications. Mutagenesis: Site directed mutagenesis and its applications. Polymerase chain reaction, RFLP, RAPD, AFLP and their applications. Southern blotting. DNA sequencing enzymatic, chemical and automated. Transformation in plants: feature of Ti and Ri plasmid, basis of tumour formation, mechanism of DNA transfer, role of virulence geness, use of reporter genes. Viral vectors and their applications, particle bombardment, electroporation, microinjection. Application of plant transformation for productivity, performance, herbicide resistance, long shelf life of fruits and vegetables. An introduction to markers, quantitative trait loci (QTL) mapping. Marker assisted selection for crop improvement. DNA chip technology and microarrays: Types and production of chips. Applications of microarrays on DNA chips. RNAi. Gene silencing and antisense technology. Gene therapy - Vectors for gene therapy, gene editing and gene replacement. Scope and applications of genetic engineering in medicine, agriculture and industry. Biosafety: Guideline for safety in biotechnology, good laboratory practice (GLP), good large scale industrial practice (GLSP), biosafety level for infectious agents. Regulations environment, environmental pollution and hazardous substance.
Recommended Books; 1. Recombinant DNA: J. D. Watson, M. Gilman, J. Witkowski and M. Zoller (Scientific American Books distriduted by W. H. Freeman and co.). 2. Principles of gene manipulation an introduction to genetic engineering: R. W. Old and S. B. Primrose (Blackwell Scientific Publications). 3. Genes: B. Lewin (Oxford University Press). 4. Biochemistry: L. Stryer (W.H. Freeman & co.). 5. Lehninger principles of biochemistry: D. L. Nelson & M. M. Cox (Macmillan Worth Publishers). 6. Cell and molecular biology: P. Sheeter and D. E. Blanchi (John Wiley & sons Inc.) 7. Methods in molecular biology, Vol. 92 PCR in bioanalysis: S. J. Metzer (Humamna press). 8. Gene function E. coli and its heritable elements: R. C. Glass (Croom Helm). 12
9. Principles of genetics: E. J. Gardner, M. J. Simmons, D. P. Snustad (John Wiley & sons Inc.). 10. Genetic engineering: R. Williamson (Academic press Inc.). 11. Molecular cloning a laboratory manual : J. Sambrook, E. F. Frithsch and T. Maniatis (Cold Spring Harbor Laboratory Press). 12. Molecular Biotechnology: S. B. Primrose (Blackwell Scientific Publication). 13. Methods in enzymology Vol. 152; guide to molecular cloning techniques (Academic Press). 14. DNA cloning a practical approach: D. M. Glover and B. D> Hames (IRL Press). 15. Gene therapy principles and applications: T. Blankenstein (Birkhauser Verlag). 16. Genetic engineering: P. K. Gupta (Rastogi publications). 17. Biotechnology and genomics: P. K. Gupta (Rastogi publications). 18. Biotechnology: R. C. Dubey (S. Chand). 19. The Indian environment protection act, 1986 (Govt. of India). 20. Rules for manufacture, use / import / export / and storage of hazardous microorganisms or cells act, 1989 (Govt. of India). 21. Food safety and standards act. 2006 (Govt. of India).
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Intellectual property rights: Patents, trade secrets, copyrights, trademarks and geographical indications. IPR and plant genetic resources. IPR for plant breeding Sui Generis system, plant variety protection and UPOV, plant variety protection in India and USA. Plant breeders rights and farmers rights.
Recommended Books; 1. Biotechnology - secondary metabolites: K. G. Ramawat and J. M. Merillon (Eds.) (Oxford & IBH publishing Co. Pvt. Ltd.). 2. Natural products - chemistry and biological significance: J. Mann, R. S. Davidson, J. B. Hobbs, D. V. Banthrope and J. B. Harbonne (Longman). 3. New trends in natural product chemistry: Ata-ur-Rahman and M. L. Choudhary (Harwood Academic Publishers). 4. Pharmacognosy: G. E. Trease and W. C. Evans (ELBS) 5. Text book of pharmacognosy: T. E. Wallis (CBS Publishers) 14
6. Organic chemistry of natural products: Gurdeep Chatwal (Himalaya publishing House). 7. Indian medicinal plants: Kirthikar and Basu 8. Materia medica: Henry G. Greenish (Scientific Publishers) 9. Agrochemicals from natural products: C. R. A. Godfrey(Ed.) (Marcel Dekker, Inc.) 10. The Total synthesis of natural products: John ApSimon (Ed.) (John Wiley & Sons, Inc.). 11. Introduction to flavonoids: B. A. Bohn (Harwood Academic Publishers). 12. Insecticides of natural origin: Sukh Dev (Harwood Academic Publishers). 13. Chemistry and biology of herbal medicine: V. P. Agrawal and V. P. Khamboj (Eds.) (Society of Biosciences). 14. Foyes Principles of medicinal chemistry: T. L. Lemke, D. A. Williams, V. F. Roche and S. W. Zito (Wolters Kluwer / Lippincott Williams & Wilkins). 15. Biopharmaceueticals - biochemistry & biotechnology: G. Walsh (Wiley).
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Recommended Books; 1. 2. 3. 4. 5. Culture of animal cell: R. I. Freshney (Wiley - Liss). Genes: B. Lewin (Pearson Educational International). Animal cell culture Practical approach: R. W. Jhon (Masters Oxford). Biotechnology: U. Satyanarayana (Books & Allied Pvt. Ltd.). Methods in cell Biology - Vol. 57 - animal cell culture methods: J. P. Mathon and D. Barnes (Eds.) (Academic Press). 16
6. Synthetic drugs: M.S. Yadav. 7. Synthetic organic chemistry: Gurudeep Chatwal (Himalaya Publishing House). 8. Foyes Principles of medicinal chemistry: T. L. Lemke, D. A. Williams, V. F. Roche and S. W. Zito (Wolters Kluwer / Lippincott Williams & Wilkins). 9. Biopharmaceueticals - biochemistry & biotechnology: G. Walsh (Wiley). 10. Biotechnology and Pharmacy: J. M. Pezzuta, M. Jhonson and H. R. Manasse.
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