POST TRANSCRIPTIONAL GENE SILENCING

BY:HINA AGRAVAL T.Y. BSC BIOTECHNOLOGY, GOVERNMENT SCIENCE COLLEGE, KHOKHRA,AHMEDABAD.

DEFINATION
Post transcriptional gene silencing is a mechanism where the presence of certain fragments of double stranded RNA interferes with the expression of a particular gene which shares a homologous sequence with this dsRNA.

Post transcriptional gene silencing is also known as RNA interference.

RNAi timeline.

2006
Nobel Prize to Andrew fire and Craig mello
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Mechanism of SiRNA

Andrew fire and craig mello RNAi is also known as gene silencing or gene knockdown or post transcriptional gene silencing.In fungi,it is also known as quelling

Slicer

Mechanism for miRNA

DICER A ribonuclease in the RNase III family

catalyzes the first step in the RNA interference pathway . helps in the cleavage of dsRNA.

CRYSTAL STRUCTURE OF DICER

RNA-induced silencing complex

The main component of RISC complex is argonaute proteins and they contain PAZ and PIWI domains.

SLICER DEPENDENT DECAY OF mRNA

SLICER INDEPENDENT DECAY OF mRNA

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APPLICATION
Highly specific Remarkably potent Investigating gene function Reducing gene expression SiRNAs as therapeutics for cancer, viral infections, parasitic infections and more. Modulation of HIV-1 replication by RNA interference. Powerful for analyzing unknown genes in sequence genomes. RNAi regulates heterochromatic silencing.
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Conclusion

Medline Citations 250 200

RNAi is so important: 150 Helps identify and study proteins 100 1. Led to cures for diseases 50 y Biological functions: 0 1998 1999 2000 2001 2002 1. Antiviral Defense 2. Regulation of gene expression DISCOVERY TO TOLLS

RNAi siRNA

Future of RNAi y Cancer research, immunology, cell biology, and neuroscience y Research for HIV virus and other diseases y Drug discovery y Lead to therapeutics
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FOR miRNA AND siRNA