Commonly used protease and phosphatase inhibitors

Inhibitor
Sodium Fluoride Sodium Orthovanadate 2-Glycerophosphate (disodium salt) Sodium Pyrophosphate AEBSFHCl Aprotinin Bestatin E-64 EDTA Leupeptin Pepstatin A PMSF

MW
42.0 183.9 216.0 221.9 239.5 6511.5 308.4 357.4 372.2 475.6 685.9 174.2

Target class
Ser/Thr and acidic phosphatases Tyr and alkaline phosphatases Ser/Thr phosphatases

Type
Irreversible Reversible Reversible

Solubility (solvent), 20 C
40 mg/ml (H2O) 20 mg/ml (H2O) 10 mg/ml (H2O) 65 mg/ml (H2O) 200 mg/ml (H2O) 10 mg/ml (H2O) 5 mg/ml (MeOH) 20 mg/ml (1:1 EtOH:H2O) 10 g/100 ml (H2O) 1 mg/ml (H2O) 1 mg/ml (MeOH) 18 mg/ml (MeOH)

Typical working concentration

1-50 mM 1-100 mM 1-100 mM 1-100 mM 0.2-1 mM 100-200 nM 1-10 M 1-20 M 2-10 mM 10-100 M 1-20 M 0.1-1 mM

Ser/Thr phosphatases Irreversible Ser proteases Ser proteases Amino-peptidases Cys proteases Metalloproteases (chelates cations) Ser/Cys proteases Asp proteases Ser proteases Irreversible Reversible Reversible Irreversible Reversible Reversible Reversible Irreversible