BIOPROCESS TECHNOLOGY

Course Code: BTB 701 Credit Units: 03 Course Objective:

The objective of the course is to apply the principles of biochemical engineering in large scale cultivation of microorganism for production of important products.

Course Contents:
Module I Advantage of bioprocess over chemical process. Basic principle in bioprocess technology. Media formulation, Cell culture techniques; Inoculum development and aseptic transfers. Different types of pumps, valves, and line materials, piping conventions etc. used in Biochemical Process Module II Process technology for the production of primary metabolites, eg. biomass, ethanol, acetone-butanol, citric acid, amino acids, polysaccharides and plastics. Ethanol: production by batch, continuous and cell recycle adopted by various technologies practiced in Indian distilleries using molasses and grains. Computation of fermentation efficiency, distillation efficiency and overall efficiency of ethanol production, recovery, uses, glucose effect etc. Power alcohol definition, uses, merits and demerits of various technologies for its production. Amino Acid: Genetic Control of metabolic pathway. Lysine: Indirect and direct fermentation mechanism of ph of metabolic block in accumulation of L- lysine by inhibition and repression mechanism. Biomass: Bakers and distillers yeast production using various raw materials, bios factors for growth, Crabtree effect, harvesting, different forms and uses. What are mushroom, different forms of common mushroom production from agro based raw materials and uses. Biofertilizers, biocompost and biopesticides Module III Production of secondary metabolites penicillin, cephalosporins, streptomycin, tetracycline etc. Metabolites from plant and animal cell culture Penicillin: Classification, various penicillin as precursor and R side chain, penicillinase, 6-APA, penicillin production, harvest and recovery, uses of various forms etc. Streptomycin: chemical structure, production, harvest and recovery, use by-product of streptomycin fermentation etc. Tetracycline: chemical structure, production, harvest and recovery, use by-product of tetracycline fermentation etc. Module IV Microbial production of industrial enzymes glucose isomerase, penicillin acylase, cellulase, amylase, lipase, protease etc.