Regulacin y Funcionalidad del gen Rnf19 en Espermatognesis

Rnf19 (XYbp)

Das Postnatales: 4

10 12 14 15 16 17 18

19 20 22 Ad

4.2 KB 2.8 KB

Actina

Promotor Rnf19

eGFP

43C
3h
EGFP P.C. EGFP

6h
P.C.

C HS 1

HS

HS 2

HS

Protena Rnf19
Rnf19 (840 aa y 90 KDa)
RING-finger Regiones de localizacin nuclear Regiones transmembrana

MQEQEISFIFKYNEGLCMNIDSDSILMSILDMSLHQQMGSDRDLQSSTSSVSLPSVK KAPKQRRISIGSLFRRKKDSKRKSRELNGGVDGIASIESIHSEMCADKNSIFSTNTSS DNGLTSISKQIGDFIECPLCLLRHSKDRFPDIMTCHHRSCVDCLRQYLRIEISESRVNI SCPECTERFNPHDIRLILSDDVLMEKYEEFMLRRWLVADPDCRWCPAPDCGYAVIA FGCASCPKLTCGREGCGTEFCYHCKQIWHPNQTCDAARQERAQSLRLRTIRSSSIS YSQESGAAADDIKPCPRCAAYIIKMNDGSCNHMTCAVCGCEFCWLCMKEISDLHYL SPSGCTFWGKKPWSRKKKILWQLGTLVGAPVGIALIAGIAIPAMIIGIPVYVGRKIHN RYEGKDVSKHKRNLAIAGGVTLSVIVSPVVAAVTVGIGVPIMLAYVYGVVPISLCRS GGCGVSAGNGKGVRIEFDDENDINVGGTNAAIDTTSVAEARHNPSIGEGSVGGLT GSLSASGSHMDRIGTIRDNLSETASTMALAGASITGSLSGSAMVNCFNRLEVQADV QKERCSLSGESGTVSLGTVSDNASTKAMAGSILNSYIPLDREGNSMEVQVDIESKP FKFRHNSGSSSVDDSGATRGHTGGASSGLPEGKSSATKWSKEATGGKKSKSGKL RKKGNMKINETREDMDAQLLEQQSTNSSEFEAPSLSDSMPSVADSHSSHFSEFSCS DLESMRTSCSHGSSDCHARFTAVNTLPEVENDRLENSPHQCSSALLSKAASCSDV PQPSHAADEHGTSRSGGKPMVDLCFGDALRETNNNHSHQTADLKVAVQTEI

Confirmacin de la funcionalidad de la construccin Red

CMV

DsRed

3UTR Rnf19 2.8 kb