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  • Protein Assay: Concentrate With 4 Parts Nano H O. Filter Through Whatman #1 (Or Equivalent) To Remove

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    Cat Doujasy
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    The document provides instructions for performing a protein assay using the Bradford method. It describes diluting the dye reagent and preparing protein standards by serial dilution between 0.1 and 0.5 mg/ml. It then instructs to pipette samples and standards into a microtiter plate, add diluted dye reagent, incubate at room temperature for at least 5 minutes, and measure absorbance at 595nm to calculate protein concentrations.

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    Protein Assay Bradford

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    The document provides instructions for performing a protein assay using the Bradford method. It describes diluting the dye reagent and preparing protein standards by serial dilution between 0.1 and 0.5 mg/ml. It then instructs to pipette samples and standards into a microtiter plate, add diluted dye reagent, incubate at room temperature for at least 5 minutes, and measure absorbance at 595nm to calculate protein concentrations.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
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    19 views1 page

    Protein Assay: Concentrate With 4 Parts Nano H O. Filter Through Whatman #1 (Or Equivalent) To Remove

    Uploaded by

    Cat Doujasy
    The document provides instructions for performing a protein assay using the Bradford method. It describes diluting the dye reagent and preparing protein standards by serial dilution between 0.1 and 0.5 mg/ml. It then instructs to pipette samples and standards into a microtiter plate, add diluted dye reagent, incubate at room temperature for at least 5 minutes, and measure absorbance at 595nm to calculate protein concentrations.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Flag for inappropriate content
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    Protein assay Bradford method

    May 7.2008

    Protein Assay
    1. Prepare dye reagent by diluting 1 Concentrate with 4 parts Nano H2O. part Dye Reagent

    2.

    Filter through Whatman #1 (or equivalent) to remove particulates. *The diluted reagent may be used for approximately 2 weeks at RT Prepare 3-5 dilutions of protein standard by 1:2 serial dilutions. 0.1, 0.2, 0.3, 0.4, 0.5 mg/ml **The linear range of the protein solution is 0.05 mg/ml to approximately 0.5 mg/ml **Protein solutions are normally assayed in duplicate or triplicate Pipette 10l of each standard & sample solution into a clean, dry microtiter plate. Add 200l of diluted dye reagent to each tube & vortex. Incubate at RT for 1 hr incubation time 5 min. *absorbance will overtime Measure absorbance at 595nm. Calculate protein concentration of the samples.

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