Tryptone Bile Agar (TBA)

TN 1260 500 g

Intended use
Used to detect and count E. coli using the membrane filtration method in water and the membrane-agar method in foodstuffs (according to 64 German Food and Feed Code).

Typical composition
Component Casein peptone (tryptone) Bile salts No. 3 Agar g/l 20 1.5 10

Form
Powder

Directions
Suspend 31.5 g in 1 l distilled water and heat until completely dissolved. Autoclave at 121C for 15 minutes.

Colour
Bright beige

Final pH at 25C
7.2 0.2

Storage
Dry, tightly closed, at 10...25C.

Shelf life
5 years

Microbiological quality control

Incubate Tryptone Bile Agar (TBA) aerobically for 22-24 hours at 44 1C. Test strain Escherichia coli Escherichia coli Escherichia coli Enterobacter cloacae Citrobacter freundii Staphylococcus aureus Pseudomonas aeruginosa Enterococcus faecalis Proteus mirabilis ATCC no. 25922 10536 8739 13047 8090 25923 25668 19433 29906 Inoculum CFU/plate 10-100 10-100 10-100 10-100 10-100 ca. 1000 10-100 ca. 1000 10-100 Growth good good good good no growth no growth no growth no growth no growth Indole reaction positive positive positive negative n. a. n. a. n. a. n. a. n. a.

Manufacturer: SIFIN Institut fr Immunprparate und Nhrmedien GmbH Berlin Berliner Allee 317-321 13088 Berlin Germany P +49-30-92 70 30-0 F +49-30-92 70 30-30 info@sifin.de www.sifin.de Date of revision: April 2006