Human T cell Development

www.abcam.com
CD10-/+ CD34 CD7-/+ CD7-/+ CD135 T cell (DN2) CD5 CD1A T cell (DN3) CD135 IL7 STATs CD4 CD8-/+ T cell (ISP) CD1A Pro T cell (DN1) CD7++ CD7++ BCL11B NOTCH1
Thymus seeding progenitor

CD10-/+

CD34

CD34

CD34

NOTCH, BCL11B, GATA3, TCF, SCF, IL7 (FLT3L)

TCR Rearrangement

TCR,- Rearrangement

TCR selection
ID3high

CD4-/+ CD8-/+

RAG1,2 NOTCH E2A HEB GATA3 PTCRA (pT) TCF1 LEF

Pre-TCR
CD4 Pre-TCR T cell (preDP) CD8-/+ CD1A CD28 CD71 CD4 / TCR CD3 CD4+ CD8+ (DP) CD8 CD1A RORT GATA3 TCF1 ID3low RUNX1 HEB

/ TCR

/ T cell

/ TCR CD3

MARROW
RAG1,2 GATA3 RUNX1 E2A HEB

TCR Rearrangement
CD56

CD4-/+

/ TCR (V24) CD8-/+ NKT cell CD1D selection on DP thymocytes SLAM/SAP signaling PLZF IL15 CD45RO CD4 MHC-II selected CD28 CD3

THYMUS

/ TCR

MHC selection
FOXP3 Treg cell CD25 CD3

No MHC affinity
MHC-II

- Selection

Intermediate MHC affinity

MHC-I Epithelial cell Epithelial cell MHC-II

+ Selection

High MHC affinity

- Selection

CD45RA CD31

Dendritic cell

Macrophage

MHC-I

CD27 CD4+8+ (DP) CD69 GATA3 TH-POK MYB

CD3 CD4+8+ (DP) CD1A

CD27 CD4+8+ (DP) CD69 IL7 STAT5 RUNX3

CD3 CD4+8+ (DP)

Apoptosis

Apoptosis
/ TCR CD8 / TCR

BLOOD
IFN, T-BET, STAT4, IL12, RUNX3 / TCR CD4 TH 1 cell CD3 IL12R CD4 TH 2 cell CD45RO CD3 CD45RO IL4, GATA3, IL10, STAT6 / TCR CD4 CD3

CD4 TH cell CD3 CD45RA IL6, RORT, IL23, IL1, STAT3 / TCR IL23R TH 17 cell CCR4 CD45RO CD161 CCR6 (CD196) TC cell

IL2

CD3 TGF, FOXP3 STAT5 / TCR CD4 CD3 CD45RO Treg cell CD127-/+ CD25 CD95 IL15 CD8 / TCR CD4 CD3 Memory cell CD45RO CD127 CD25 CD62L Memory cell CD45RO / TCR CD3 Effector cell CD45RA CD8 / TCR CD3

Copyright 2010 Abcam, All Rights Reserved. Produced in collaboration with Dr. Tom Taghon, Ghent University, Belgium.

Related antibodies from Abcam:

Product Clonality Applications Host Species Reactivity
Hu, Hu, Hu Hu Hu Hu Hu, Hu Hu Hu, Hu, Hu Hu Hu Hu Hu Hu Hu Hu Hu, Hu, Hu Hu Hu Hu, Hu Hu Hu Hu Hu Hu, Hu Hu, Hu, Hu Hu Hu, Hu Hu Hu Hu Hu, Hu Hu Hu Hu Hu, Hu, Hu, Hu Hu Hu, Hu, Hu Hu, Hu, CynMk Ms, Rat

See more products at: www.abcam.com

Datasheet www.abcam.com/ab
1669 78429 951 708 95732 42742 16044 32457 63985 51312 41478 27290 23 52068 95609 27324 95595 71530 59233 28448 86002 69758 22831 32858 41834 2105 95528 96517 55282 92381 53656 9622 6672 73341 9657 74549 9018 65297 39354 95955 74703 35962 40278 56796 52043 57822 32364 44718 53174 57680 59117 18861 18862 20985 56250 1086

Featured antibodies

Ms, Dog Rat

144-09/10-PW

CCR4 CCR6 CD10 CD1a CD1d CD28 (FITC) CD3 CD31 CD34 CD4 CD44 CD45RA (FITC) CD45RO CD62L (FITC) CD69 (Allophycocyanin) CD7 (Phycoerythrin) CD8 alpha (PE/Cy5) CD8 beta - Aminoterminal end c-Myb Ctip2 ELK4 Flt3 ligand FOXP3 (HRP) GATA3 ID3 IL1 beta IL12 (Phycoerythrin) IL12RB1 IL15 IL2 IL23 Receptor - Aminoterminal end IL4 IL6 IL7R alpha (phospho Y449) Interferon gamma LEF1 NCAM Notch1 Plzf RAG2 ROR gamma RUNX1 / AML1 RUNX3 SOX13 SOX4 STAT4 STAT5 (phospho Y694) STAT6 T-bet / Tbx21 TCF12 TCF3 / E2A TCR alpha TCR beta ThPok TOX1 Transferrin Receptor

P P M M M M P P P M P M M M M M M P P P P P M P P P M P M M P P P P P P M P P P P P M M P M M P P M P P P P P M

ELISA, ICC, ICC/IF IHC-Fr, IHC-P WB , , IHC-P WB , IHC-Fr, IHC-P WB , IHC-Fr, IHC-P Flow Cyt Flow Cyt, IP WB , IHC-Fr, IHC-P WB , Flow Cyt, IHC-P WB , WB IHC-P WB , IHC-P WB , Flow Cyt Flow Cyt, IHC-Fr, IHC-P IP WB , , Flow Cyt, FuncS Flow Cyt Flow Cyt Flow Cyt ELISA, WB ELISA, IHC-P IHC-Fr, IHC-P WB , ELISA, WB ELISA, WB IHC-P WB , IHC-P WB , IHC-Fr, IHC-P ELISA, Flow Cyt, FuncS, IHC-Fr, IHC-P IP RIA, WB , , Flow Cyt WB IHC-P ICC, IHC-P WB , ICC, IHC-P WB , ELISA, IHC-P Neut, WB , ELISA, ICC/IF IHC-Fr, IHC-P IP Neut, RIA, WB , , , ELISA, ICC/IF WB , ELISA, IHC-P Neut, WB , WB ICC, ICC/IF IF IHC-Fr, WB , , IHC-P WB , WB ICC/IF IHC-P WB , , WB IHC-P WB , EMSA, IHC-P IP WB , , WB IHC-P ICC/IF IHC-P WB , , ICC/IF IHC-P IP WB , , , ICC, ICC/IF IHC-Fr, IHC-P IP WB , , , WB IHC-P WB , ELISA, IHC-P WB , IHC-Fr, IHC-P IP WB , , IHC-Fr, IP WB , ICC/IF WB , WB Flow Cyt, IP WB ,

Gt Rb Ms Ms Ms Ms Rb Rb Rb Ms Rb Ms Ms Ms Ms Ms Ms Rb Rb Rb Rb Rb Ms Rb Rb Rb Ms Rb Ms Rb Gt Rb Rb Rb Rb Rb Ms Rb Rb Rb Rb Rb Ms Ms Rb Ms Rb Rb Rb Ms Gt Rb Rb Rb Rb Ms

CD95 (ab2437)
Clonality Applications P IHC-Fr, IHC-P IP WB , , Host Rb Species cross reactivity Hu

Ms

Ms Ms, Rat

c-Kit (ab5506)
Clonality Applications P ELISA, ICC/IF IHC-Fr, IHC-P WB , , Host Rb Species cross reactivity Hu, Ms

Ms, Rat Ms

Ms

IRF1 (ab26109)
Clonality Applications P ICC/IF IHC-P WB , , Host Rb Species cross reactivity Hu

Ms Ms, Rat, Pig Ms

Ms, Rat

SCF (ab64677)
Clonality Applications P ICC/IF IHC-P WB , , Host Rb Species cross reactivity AGMk, Hu, Ms

Ms

Ms Ms, Rat Ms

TGF beta 2 (ab36495)

Clonality Applications M IHC-Fr, IHC-P WB , Host Ms Species cross reactivity Hu, Ms, Cow

Ms, Rat Ms, Rat