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  • Half Life Measurement of Budding Yeast Proteins

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    Li Sun
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    The document presents a table showing the results of measuring the half-life of 151 yeast proteins in minutes. For each protein, the table lists the ORF name, gene name if known, and the measured half-life in minutes for two repeated measurements. The half-lives ranged from 2 to over 400 minutes. The repeat measurements were generally similar but in some cases varied, demonstrating the repeatability of the half-life measurement technique.

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    The document presents a table showing the results of measuring the half-life of 151 yeast proteins in minutes. For each protein, the table lists the ORF name, gene name if known, and the measured half-life in minutes for two repeated measurements. The half-lives ranged from 2 to over 400 minutes. The repeat measurements were generally similar but in some cases varied, demonstrating the repeatability of the half-life measurement technique.

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    8 views4 pages

    Half Life Measurement of Budding Yeast Proteins

    Uploaded by

    Li Sun
    The document presents a table showing the results of measuring the half-life of 151 yeast proteins in minutes. For each protein, the table lists the ORF name, gene name if known, and the measured half-life in minutes for two repeated measurements. The half-lives ranged from 2 to over 400 minutes. The repeat measurements were generally similar but in some cases varied, demonstrating the repeatability of the half-life measurement technique.

    Copyright:

    Attribution Non-Commercial (BY-NC)
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    of 4

    Table 1.

    Half-life measurement for a random sample of 151 proteins used


    to establish half-life measurement repeatability
    ORF
    YGL252C
    YGR204W
    YLR103C
    YNL127W
    YJL069C
    YAL005C
    YDR101C
    YDR194C
    YDL174C
    YGL234W
    YLR289W
    YHL007C
    YER103W
    YPR019W
    YML056C
    YGL234W
    YDL190C
    YOR335C
    YOR076C
    YDR243C
    YLR427W
    YJL209W
    YLR028C
    YOL113W
    YLL026W
    YIL137C
    YHR015W
    YPR190C
    YML082W
    YPR135W
    YGL107C
    YJL154C
    YPR141C
    YMR277W
    YDR356W
    YPR091C
    YPL040C
    YGR246C
    YIL017C
    YGL125W
    YMR125W
    YML054C
    YKL185W
    YBR260C
    YML042W
    YIL130W
    YOL025W
    YIL075C

    Gene name
    RTG2
    ADE3
    CDC45

    SSA1
    MSS116
    DLD1
    ADE5,7
    GUF1
    STE20
    SSA4
    CDC54
    IMD4
    ADE5,7
    UFD2
    ALA1
    PRP28
    CBP1
    ADE16
    SKM1
    HSP104
    MIP6
    RPC82
    CTF4
    VPS35
    KAR3
    FCP1
    NUF1
    ISM1
    BRF1
    VID28
    MET13
    STO1
    CYB2
    ASH1
    RGD1
    CAT2
    LAG2
    RPN2

    First HL,
    minutes
    70
    81
    66
    97
    85
    119
    168
    86
    137
    80
    84
    98
    100
    61
    300
    300
    105
    300
    20
    31
    24
    43
    300
    20
    59
    37
    2
    26
    107
    51
    52
    57
    29
    18
    33
    56
    55
    24
    7
    300
    54
    64
    3
    51
    41
    3
    31
    52

    Second HL,
    minutes
    69
    86
    78
    84
    60
    77
    96
    162
    61
    162
    300
    300
    300
    300
    65
    162
    300
    108
    20
    31
    24
    43
    59
    21
    61
    36
    2
    27
    58
    49
    55
    320
    27
    20
    36
    51
    300
    26
    7
    54
    65
    54
    2
    45
    47
    3
    36
    400

    YCR084C
    YHR120W
    YGL060W
    YKL005C
    YIL155C
    YNL118C
    YBR169C
    YMR287C
    YOL117W
    YPR008W
    YGL227W
    YOR350C
    YML109W
    YDL227C
    YOL090W
    YPL084W
    YKR036C
    YMR019W
    YKL134C
    YJL057C
    YGR162W
    YJL128C
    YCR088W
    YIL101C
    YPL007C
    YNL008C
    YLR060W
    YKR089C
    YER075C
    YFR040W
    YNL218W
    YDL220C
    YMR227C
    YOL066C
    YBR017C
    YGL113W
    YER049W
    YIL056W
    YAR014C
    YMR120C
    YGR287C
    YPR179C
    YER088C
    YKR002W
    YGR100W
    YKL135C
    YCR038C
    YLR234W
    YML023C
    YPR111W
    YLR369W
    YNL047C
    YDL113C
    YKL048C
    YOR207C
    YDR389W

    TUP1
    MSH1

    GUT2
    DCP2
    SSE2
    MSU1

    VID30
    MNE1
    ZDS2
    HO
    MSH2
    BRO1
    CAF4
    STB4
    IKS1
    TIF4631
    PBS2
    ABP1
    XBP1
    TFC8
    FRS1
    PTP3
    SAP155
    CDC13
    TAF67
    RIB2
    KAP104
    SLD3

    BUD14
    ADE17
    PLO1
    DOT6
    PAP1
    MDR1
    APL2
    BUD5
    TOP3
    DBF20
    SSQ1

    ELM1
    RET1
    SAC7

    32
    52
    53
    19
    98
    43
    50
    42
    43
    20
    48
    39
    83
    3
    25
    34
    44
    2
    48
    16
    32
    48
    14
    26
    71
    271
    300
    23
    26
    300
    32
    60
    38
    164
    99
    11
    23
    39
    19
    91
    39
    19
    29
    38
    37
    37
    36
    45
    22
    26
    35
    29
    32
    25
    44
    28

    27
    78
    45
    22
    51
    36
    5,252
    35
    51
    17
    62
    31
    47
    2
    32
    27
    34
    3
    37
    21
    42
    36
    18
    19
    44
    44
    44
    17
    38
    42
    22
    42
    55
    42
    41
    16
    35
    26
    29
    39
    4,816
    30
    18
    23
    106
    71
    122
    27
    36
    16
    61
    49
    55
    42
    25
    16

    YKL032C
    YPL086C
    YOR147W
    YDR137W
    YDR294C
    YAL011W
    YLR117C
    YNL023C
    YEL032W
    YDR144C
    YNL308C
    YBR212W
    YPL263C
    YDR307W
    YGR140W
    YBR123C
    YOR308C
    YLR299W
    YMR288W
    YNL094W
    YNL197C
    YMR080C
    YLR002C
    YGR068C
    YGR250C
    YNL236W
    YBR073W
    YLR429W
    YJL127C
    YMR224C
    YOR154W
    YIL015W
    YOR299W
    YGL066W
    YOR349W
    YNL039W
    YEL007W
    YGR186W
    YFR048W
    YHR149C
    YNL082W
    YGL099W
    YBR289W
    YOR363C
    YER032W
    YBR094W
    YKL112W
    HL, half-life.

    IXR1
    ELP3
    RGP1
    DPL1
    CLF1
    FAP1
    MCM3
    MKC7
    KRI1
    NGR1
    KEL3
    CBF2
    TFC1
    SNU66
    ECM38
    HSH155
    WHI3
    NAM7

    SIN4
    RDH54
    CRN1
    SPT10
    MRE11
    BAR1
    BUD7
    CIN1
    TFC5
    TOS9
    TFG1

    PMS1
    KRE35
    SNF5
    PIP2
    FIR1
    ABF1

    17
    55
    72
    118
    87
    30
    69
    31
    130
    115
    300
    56
    95
    300
    29
    47
    47
    8
    460
    27
    10
    42
    19
    60
    85
    24
    12
    60
    60
    18
    91
    2
    20
    69
    53
    70
    37
    10
    15
    110
    32
    3
    60
    76
    235
    127
    205

    10
    32
    32
    32
    32
    56
    31
    89
    31
    31
    31
    28
    29
    29
    101
    22
    22
    18
    27
    64
    22
    18
    44
    25
    24
    300
    30
    23
    22
    7
    21
    7
    62
    20
    16
    13
    8
    290
    2
    9
    2
    44
    3
    3
    3
    2
    2

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