Common g chain

Activating FC receptors (ITAM)

Immunoreceptor tyrosine-based activation motif

Inhibitory FC receptors (ITIM)

Immunoreceptor tyrosine-based inhibition motif

V/I/xYxxL/V

Transporter FC receptors
plyR (polymeric Ig receptor) secretory antibodies IgA FcRn neonatal passive immunity, transport milk IgG across gut epithelial cells to blood vessels

FcgRIIA

FcRn

IgG pH7

FcRn IgG <pH7

While IgG is transported by FcRn from stomach across epithelial to blood or tissues of the baby. IgA antibodies stay in the intestinal track to provide immune protection.

When a baby acquires a passive immunity (IgA) from his mother in milk by breast feeding, why IgA in milk doesn't undergo degradation & destruction in the stomach of the baby?
Slow degradationHalf-life 2 days hinge region of the monomers as well as the J-chain are heavily glycosylated protease can not interact with the antibody

What would you expect, if a person has IgA deficiency ?

After Germinal Center Reaction, B cells with better receptors and various isotypes will become .....

Memory B cells
CD40
After GC reaction sustained CD40 signaling Bcl-6 expressionI Blimp-1

Plasma cells (Blimp-1, XBP-1)

Long-lived plasma cells After GC reaction deprive CD40 signaling plasma cell terminal differentiation Short lived plasma cells B cell activation without T cell help IgM (or IgG3), low affinity (with SHM, but no affinity maturation) (T independent response by B1 cells or marginal zone B cells) Natural antibodies

How V(D)J recombination was discovered?

The End of Classic Immunology

Susumu Tonegawa (1976): Discovery of V(D)J gene segments in generation of antibody diversity
B cell
Myeloma Myeloma

V probe

C probe

The beginning of the molecular immunology............

We do we need to express Pre-B cell receptor?

Ligand-independent

heavy chain rearrangement

further heavy chain rearrangement Light chain rearrangement

VpreB

l5

Why FACS analysis is useful?

WT MT 0.5

B220

IgM+,IgD+ Recirculating B cells

Immature B cells

IgM
Pre B cells Pro B cells

CD43

The major stages in B cell development

Where does the B cell come from ............

B cell receptor Receptor editing

Encounter Antigen T cell help Germinal center Reaction Somatic hypermutation Class switch Affinity maturation

Heavy chain Light chain V(D)J recombination V(D)J recombination Pre-BCR Allelic exclusion

Memory B cells

Plasma cells