Dng 3 b nh, 1 b cha 12 con ngu nhin , 3 con trong tng replicate of mi treatment, dung lm negative control Ngy

6/6: Tra 2h v chiu 5h khng cho n Ngy 7/6: Cn c v o c Ngy 8/6: Adjust pH to 9, cho n, sc oxy, Ngy 9 -10/6: Maintain pH at 9, khng cho n, nhng c sc oxy, Ngy 11, 12, 13, 14: Shock, khng cho n, khng cho sc oxy Ngy 15: Thu kt qu c cht. B cha nc: Ngy 10 thng 6, fill nc y b 750L. Cho sc kh oxy mnh n khi nc bo ha. Sng ngy 11-14/6, lc 7h30 dzo trng, cho thm NH4Cl v khng cho sc kh. Siphon nc mi b ra gn ht, Adjust pH khong 9h v 4h, Dng NaOH iu chnh pH, khi pH qu cao, siphon 1 t nc ra v thay th bng nc ging. Sau khi dung ht nc trong b 750L, lp tc fill nc, cho sc kh oxy mnh v lp li qu trnh. Sng ngy 14/6, khi dng nc trong b 750L fill nc cho cc b nh, lp tc fill nc tr li, dng vi trung ha li nc.