QUESTIONS FOR IMMUNOLOGY EXAM 1. a) b) c) d) e) 2. a) b) c) d) e) 3. a. b. c. d. e.

Somatic recombination of antibody genes: Begins by recombining V to D segments on both heavy chain alleles Results in the productive rearrangement of VDJ on both alleles of the heavy chain Utilizes the lymphocyte-specific enzymes RAG1 and RAG2 Occurs during the antigen-dependent stage of B cell development Fails to occur if the BCR recognizes self antigen in the bone marrow The antibody repertoire is diversified at the isotype level: By mechanisms involving both mRNA splicing and somatic recombination By resting, nave B cells secreting IgG, IgE and IgA Upon BCR binding to antigen in the bone marrow In response to CD8-B cell interactions By expression of both membrane and secreted forms of the antibody What is not involved in stabilizing MHC class I molecules? invariant chain calnexin calreticulin tapasin peptide

4. Which statement is false. The BCR and the TCR: a) Are encoded by gene segments that recombine to form unique specificities b) Have regions of high variability that are a result, in part, of somatic mutations and the AID enzyme c) Contain Ig folds consisting of -barrel structures d) Contain hypervariable regions created by the insertion and deletion of nucleotides at the joining junctions of variable gene segments e) Many copies of a unique BCR or TCR, respectively, are expressed on the surface of each B cell and T cell respectively 5. a) b) c) d) e) 6. a) b) c) d) e) The major advantage of the MHC locus being polymorphic is: A greater number of peptides can be presented to activate T cells Many more TCR genes are created through V(D)J recombination The B cell repertoire is highly expanded NK cell activity is increased There is less of an inflammatory response immediately upon pathogen infection Why is it important that two separate pathways of antigen presentation exist in the cell? They allow for activation of either CD4+ T cells or B cells They segregate self peptides from viral and bacterial peptides They define a pathway for activation of either CD8+ or CD4+ T cells They allow for the activation of either the innate or adaptive immune response Peptides from different pathways will activate either TH1 or TH2 cells