To question of creating of experimental models, coded by abstract rlung, mkhris, badgan thermins in Traditional medicine

M.Ambaga, A.Tumen-Olzii (Mongolia, New medicine /NCM/-medical institute,Ulanbaatar)

Introduction. Previously, we confirmed that the appearing of abstract theory of Rlung,Mkhris,Badgan in Tradititional medicine 2 thousand years ago follow from the functioning of membrane-redoxy potentials three state line systems in the first compartment with close relationship with fourth and second, third compartments in human body. We also reveal that the functional and structural unit of living cells,as membrane-redoxy potentials three state line systems in the first compartment with close relationship with fourth and second, third compartments in human body reflected in the theory of Tibetian- Mongolian Traditional medicine in the following philosophical context as wind- like mobile,unoily,light nature-external characteristics of human body,symbolized by rlung abstract thermin, sun-like hot,hot oily nature-external characteristics of human body, symbolized by mkhris abstract thermin, moon-like cool, heavy, cold oily nature-external characteristics of human body, symbolized by badgan abstract thermin. To confirm the our conception as that the appearing of abstract theory of Rlung,Mkhris,Badgan in Tradititional medicine 2 thousand years ago- follow from the functioning of membrane-redoxy potentials three state line systems in the first compartment with close relationship with fourth and second, third compartments in human body we are conducting the our study in experimental animals,during which planned observe following relationship as : a.The association between alteration in the level of fluid alpha states,consisting of unsaturated fatty acids with high levels of oxy potentials and with high levels of proton,electrons conductance and high levels heat energy release and sun-like hot,hot oily nature-external characteristics ,symbolized by mkhris abstract thermin. b. The association between alteration in the level of solid betta state,consisting of mainly saturated fatty acids, conditioning a high levels of red potentials and with slow levels of proton,electrons conductance and low levels of heat energy release, high degree of energy accumulation and high degree of high energy phosphate-ATP, high energy electrons NADPH and moon-like cool, heavy, cold oily nature-external characteristics,symbolized by badgan abstract thermin. c. The association between alteration in the level of gamma state, consisting of decreased contents of saturated-unsaturated fatty acids, conditioning a decreased levels of redoxy potentials and with slow levels of proton, electrons conductance and low levels of heat energy release and energy accumulation and low degree of high energy phosphate-ATP, high energy electrons NADPH and wind- like mobile, unoily, light natureexternal characteristics, symbolized by rlung abstract thermin

Experimental protocols.
Basic methods of investigation. Experimental justification of basic conception of NCM medicine are conducted in followinfg steps. Subjects of our experimental works was rabbits aged 18-24 months, including male and female, ratio 50:50. A.Experimental inducing of fluid alpha states,consisting of unsaturated fatty acids with high levels of oxy potentials and with high levels of proton,electrons conductance and high levels heat energy release in a rabbits was caused by injection of Dinitrophenol 2-4 (3,5 g/ kg, four days,under cutaneous ) .

Dinitrophenol 2-4 - mechanism of action is by inhibition of the F0-F1 ATP synthase molecule, located in the inner membranes of each mitochondrion. The electron transport chain still functions to pump hydrogen ions into the intermembrane space, but the coupling of the proton gradient to ATP production is rendered impossible by DNP. As a result, ATP production is dramatically reduced, and the energy is instead thrown off as heat... DNP is lipophilic weak acids that therefore readily pass through membranes,they bind protons in the acidific side of the membrane-intermembrane spaces ,diffuse through , and release them on the alkaline side-matrix side ,thereby dissipating the proton gradient metabolic rate is increased.. (D.Voet,Biochemistry,p.553).
Effects of 2,4-dinitrophenol on lipoxygenase activity and fatty acid constituents of membrane lipids in pericarp of harvested longan fruits.Chen Lian; Lin HeTong; Chen YiHui; Lin YiFen; Chen ShaoJun Journal of Tropical and Subtropical Botany 2009 Vol. 17 No. 5 pp. 477-482 :
The effects of 2,4-dinitrophenol (DNP, a uncouple agent for respiratory) on lipoxygenase (LOX) activity, fatty acid constituents in membrane lipids and cellular membrane permeability in harvested longan (Dimocarpus longan Lour. 'Fuyan') pericarp were investigated and the relationship to pericarp browning was analysed. The results showed that the cellular membrane permeability, LOX activity and browning index increased treated with DNP, the saturated fatty acids, such as palmitic acid (C16:0) and stearic acid (C18:0) increased, and the unsaturated fatty acids, such as linoleic acid (C18:2), linolenic acid (C18:3) and (C20:1), and index of unsaturated fatty acids (IUFA) and unsaturation degree of fatty acids decreased. It suggested that the pericarp browning of longan induced by DNP might be due to increasing LOX activity, enhancing degradation of membrane unsaturated fatty acid and reducing integrity of cellular membrane, which it lead to finally the contact of phenolase with phenolic substrates to produce browning polymers.

5-Lipoxygenase (5-LO) catalyzes two steps in biosynthesis of leukotrienes (LTs), a group of lipid mediators of inflammation derived from arachidonic acid (AA). LT antagonists are used in treatment of asthma; more recently a potential role also in atherosclerosis has raised considerable interest. Furthermore, possible effects of 5-LO metabolites in relation to tumorigenesis have emerged. Thus, an understanding of the

biochemistry of this lipoxygenase has potential implications for treatment of various diseases. Leukotrienes (LTs) are inflammatory mediators causing, for example, phagocyte chemotaxis and increased vascular permeability In leukotriene biosynthesis 5lipoxygenase (5-LO) catalyzes oxygenation of arachidonic acid (AA) to 5(S)hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid (5-HPETE), and further dehydration to the allylic epoxide leukotriene A4 (1). As one of six human lipoxygenases, 5-LO is expressed primarily in various leukocytes: polymorphonuclear leukocytes (neutrophils and eosinophils), monocytes/macrophages, mast cells, Blymphocytes, dendritic cells, and foam cells of human atherosclerotic tissue. LTA4 is further converted by LTA4 hydrolase to the dihydroxyacid LTB4, and by LTC4 synthases to the glutathione conjugate LTC4. The other cysteinyl-LTs are formed by hydrolytic removal of -Glu and Gly from LTC4 (yielding LTD4 and LTE4). In proinflammatory contexts, LTs typically stimulate cellular responses, which are quick in onset and of short duration (as smooth muscle contraction, phagocyte chemotaxis, increased vascular permeability). These are mediated via G-protein coupled receptors, BLT1/2 for LTB4 and CysLT1/2 and GPR17 for the cys-LTs.
B.Experimental inducing of solid betta state,consisting of mainly saturated fatty acids, conditioning a high levels of red potentials and with slow levels of proton,electrons conductance and low levels of heat energy release, high degree of energy accumulation and high degree of high energy phosphate-ATP, high energy electrons in a rabbits was caused by injection of Rotenon (2,5 g/ kg, four days,under cutaneous ) . Rotenone blocks cytochrome oxidase (complex 4) and prevents both coupled and uncoupled respiration with all substrates, including NADH, succinate and ascorbate + TMPD. Rotenone inhibits the oxidation of NADH to NAD, blocking the oxidation by NAD of substrates such as glutamate, alpha-ketoglutarate, and pyruvate. Rotenone inhibits the mitochondrial respiratory chain between diphosphopyridine nucleotide and flavine. Rotenone is a powerful inhibitor of mitochondrial electron transport (Goodman, 1985). First step: Take from all animals a blood example and separate erythrocyte populations of cells. Second step: Cell materialls are divided in 3 basic parts,consisting first compartment and second compartment, also fourth compartmemt. First compartment (hemolysate) of erythrocytes are obtained by causing hemolysis with following centrifuging, separating fluid upper part of supernatant,containing redoxy line systems. After separating hemolysate of erythrocyte all membrane systems, representing fourth compartment also are taken by washing and centifuging remnant parts. Third step:

To identification of basic membrane states and redoxy potentials of hemolysate, representing first compartment, containing redoxy line systems are used following parameters: a.Reducing capacity of of hemolysate, established by using the nitroblue tetrazolium test b.The protonizing, radical scavenging activity of hemolysate , evaluated against 2,2-

diphenyl-1-picrylhydrazyl radical. The protonizing, radical scavenging activity expressed as IC50 .

c.Contents of Deprotonized products of hemolysate ,accounted by concentration of TBA positive products. d.Oxidizing capacity of of hemolysate by using the oxidase disk test Fourth step: To identification of basic membrane states and redoxy potentials of all membrane system,representing fourth compartmemt are used following parameters: a.Reducing capacity of all membrane system,representing fourth compartmemt by using the nitroblue tetrazolium test b. The protonizing, radical scavenging activity of all membrane system,presenting fourth compartmemt hemolysate, evaluated against 2,2-diphenyl-1-picrylhydrazyl radical. The

protonizing, radical scavenging activity expressed as IC50 .

c.Oxidizing capacity of all membrane system, presenting fourth compartmemt by using the oxidase disk test d.Contents of Deprotonized products of all membrane system, representing fourth compartmemt determined by concentration of TBA positive products. e. Proportion of saturated and unsaturated fatty acids contained in LPHD and LPLD, incorporated in integrated membrane-serum circultion systemi.e. constantly circulated between all cell membrane structures and serum (second compartment ) medium are measured by using Humalyser apparatus . f. Degree of saturated and unsaturated fatty acids dependant resistanse of all membrane system, presenting fourth compartmemt are evaluated by using osmotic and pereoxidation hemolysis test. Fifth step: a. Redoxy potentials of second compartment of animals, calculated by parameters of AO:LPO system, contents of LPHD and LPLD b. Reducing capacity of second compartment of animals, established using the nitroblue tetrazolium test. c. The protonizing, radical scavenging activity of of second compartment of animals,

evaluated against 2,2-diphenyl-1-picrylhydrazyl radical. The protonizing, radical scavenging activity expressed as IC50.
d. Contents of Deprotonized products of second compartment of animals, accounted by concentration of TBA positive products. e. Contents of cholesterols, triglycerids and LPHD, LPLD. f. Oxidizing capacity of second compartment of animals, established by using the oxidase disk test

Results of investigation First results: A.Change of oxidizing activity of hemaglobine molecules in the first compartment during of modelling of high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process ) and their relationship with abstract Mkhris code. We revealed that in the case of modelling of high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process ) are increased the oxidizing activity of hemaglobine molecules in 3,0-7,67 times in comparision with control animals after 3, 7, 14 days of modelling, which is on of appearanse of a sun like hot, hot oil external characteristics, coded by abstract mkhris thermin. B.Change of reduction :oxidation ratio activity of second compartment (serum oxidase enzyme activity) during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process) and their relationship with abstract Mkhris code. By us established that in the case of modelling of high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenol mediated proton loss process) are increased the reduction :oxidation ratio activity of second compartment (serum oxidase enzyme activity) in 1,2-1,6 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearanse of a sun like hot,hot oil external characteristics ,coded by abstract mkhris thermin. C.Change of the free radical protonizing activity of membrane-redoxy 3 state line systems, existing in the first compartment) during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process) and their relationship with abstract Mkhris code. It was clear that in the case of modelling of high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process ) are slowed the free radical protonizing activity of membrane-redoxy 3 state line systems ,existing in the first compartment) in 1,1-1,12 times in comparision with control animals after 3, 7, 14 days of modelling,which is on of appearanse of a sun like hot, hot oil external characteristics ,coded by abstract mkhris thermin. D.Change of the quantity of deprotonized products in the second compartment during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process) and their relationship with abstract Mkhris code.

We revealed that in the case of modelling of high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process) are increased the quantity of deprotonized products in the second compartment in 1,2 times in comparision

with control animals after 3, 7, 14 days of modelling,which is on of appearance of a sun like hot, hot oil external characteristics, coded by abstract mkhris thermin. E.Change of the quantity of deprotonized products in the fourth compartment during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process ) and their relationship with abstract Mkhris code. It was clear that in the case of modelling of high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process ) are increased the quantity of deprotonized products in the fourth compartment in 1,2 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearanse of a sun like hot,hot oil external characteristics ,coded by abstract mkhris thermin. I.Change of the quantity of deprotonized products in the fourth compartment during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process ) and their relationship with abstract Mkhris code. By us revealed that in the case of modelling of high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process ) are increased the resistance of membrane structures ,belonging to fourth compartment in 1,18 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearanse of a sun like hot,hot oil external characteristics ,coded by abstract mkhris thermin.

Second results: A. Change of oxidizing activity of hemaglobine molecules in the first compartment during of modelling of membrane high betta state-high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) and their relationship with abstract Badgan code. We revealed hat in the case of modelling of high solid betta state mediated high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) are decreased the oxidizing activity of hemaglobine molecules in 1,8-14,0 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil external characteristics ,coded by abstract badgan thermin. B. Change of oxidizing activity of hemaglobine molecules in the first compartment during of modelling of membrane high betta state-high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) and their relationship with abstract Badgan code.

By us established that in the case of modelling of high solid betta state mediated high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) are decreased the oxidizing activity of hemaglobine molecules in 1,8-14,0 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil external characteristics ,coded by abstract badgan thermin. C.Change of reduction :oxidation ratio activity of second compartment (serum oxidase enzyme activity )during modelling of membrane high betta state-high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) and their relationship with abstract Badgan code. It was clear that in the case of modelling of membrane high betta state-high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) are decreased the reduction :oxidation ratio activity of second compartment (serum oxidase enzyme activity ) in 1,6-7,25 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil external characteristics ,coded by abstract badgan thermin. D. Change of oxidizing activity of hemaglobine molecules in the second compartment during of modelling of membrane high betta state-high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) and their relationship with abstract Badgan code. We observed that in the case of modelling of high solid betta state mediated high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) are decreased the the deprotonized products in the second compartment in 1,2 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil external characteristics ,coded by abstract badgan thermin. E. Change of deprotonized products in the forth compartment during of modelling of membrane high betta state-high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) and their relationship with abstract Badgan code. By us established that in the case of modelling of high solid betta state mediated high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) are decreased the deprotonized products in the forth compartment in 1,3 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil external characteristics ,coded by abstract badgan thermin. I. Change of the free radical protonized activity of membrane-redoxy 3 state line systems in the first compartment and membrane structures in the forth compartment during of modelling of membrane high betta state-high reduction potential elevated

situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) and their relationship with abstract Badgan code. We observed that in the case of modelling of high solid betta state mediated high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) are increased the free radical protonized activity of membrane-redoxy 3 state line systems in the first compartment and membrane structures in the forth compartment in 1,3 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil external characteristics ,coded by abstract badgan thermin. L. Change of the resistance of membrane structures ,belonging to the forth compartment during of modelling of membrane high betta state-high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) and their relationship with abstract Badgan code. It was clear that in the case of modelling of high solid betta state mediated high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) are increased the resistance of membrane structures ,belonging to the forth compartment in 1,2 times in comparision with control animals after 3,7,14 days of modelling,which is on of appearance of a moon like cold, cool oil external characteristics ,coded by abstract badgan thermin. M. Change of some parameters in the all 4 compartments during of modelling of membrane high gamma state-slow redoxy potential situation in rabbits long time use of rotenon (by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) and their relationship with abstract Badgan code. By us established that in the case of modelling of high solid betta state mediated high reduction potential elevated situation in rabbits by long time use of rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) are increased the contens of LPLD,cholesterols,triglycerids i.e.decline of dilution effect of LPHD in relation to LPLD,cholesterols,triglycerids and decreased the contens of LPHD in the the second compartment in 1,2 times in comparision with control animals after 28 days of modelling,which is on of appearance of a moon like cold, cool oil external characteristics ,coded by abstract badgan thermin. Third results: A. Change of some parameters in the all 4 compartments during of modelling of membrane high gamma state-slow redoxy potential situation in rabbits long time use of dinitrophenol (dinitrophenolmediated proton loss process ) and their relationship with abstract Rlung code. We established that in the case of modelling of membrane high gamma state-slow redoxy potential situation in rabbits long time use of dinitrophenol (dinitrophenolmediated proton loss process ) are decreased the ATP concentration by uncoupling process,because of

this decreased the ATP-ase activity ,leading to decline of slip redox potential , evidence of these was the decrease of glucose concentration in the second compartment,decline of concentration of LPLD , LPHD in 1,6 times in comparision with control animals after 14 days of modelling,which is on of appearance of a wind like unoily, mobile ,light external characteristics ,coded by abstract rlung thermin. Tab 1. Change of the intensity of NADPH generation during of modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling).

1 2 3

Study groups Control animals Dinitrophenol administrated animals Rotenon administrated animals

Content of NADPH in the first compartment 2.661+_0,014 2.407+_0,013 2.755+_0,017

Tab 3. Change of the oxidizing activity of hemaglobine molecules during of modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling). Study groups The oxidizing activity of hemaglobine molecules in the first compartment (minute ) 1 Control animals 14.75+_0,024 2 Dinitrophenol administrated animals 7.67+_0,018 3 Rotenon administrated animals 17.75+_0,038 Tab 4. Change of the reduction oxidation ratio(serum oxidase enzyme activity) in the second compartment during of modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling). Study groups The reduction oxidation ratio(serum oxidase enzyme activity) in the second compartment (minutes ) 1 Control animals 4.25+_0,024 2 Dinitrophenol administrated animals 2,0+_0,014 3 Rotenon administrated animals 7.25+_0,039 Tab 6. Change of the protonizing activity of membrane-redoxy 3 state line system during of modelling of membrane high betta state-high reduction potential

elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling ). Study groups Abs IC50 1 Control animals 0.097 77.65 2 Dinitrophenol administrated animals 0.087 79.95 3 Rotenon administrated animals 0.095 78.11 Tab 7. Change of free radical protonizing activity of membrane-redoxy 3 state line system ,located in the first compartment during modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling). Study groups Abs IC50 1 Control animals 0.197 54.61 2 Dinitrophenol administrated animals 0.204 52.99 3 Rotenon administrated animals 0.197 54.61 Tab 8. Change of contents of deprotonized products in the second compartment (serum TBA-positive products )during modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling).

Study groups

1 2

Control animals Rotenon administrated animals

The contents of de protonized products in the second compartment 0.058+_0,0014 0.033+_0,0018

Tab 9. Change of contents of deprotonized products in 5 membrane structures,belonging to the fourth compartment (serum TBA-positive products )during modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling ). Study groups The contents of deprotonized products in 5 membrane structures,belonging to the fourth compartment (serum TBApositive products ) 0.044+_0,0018 1 Control animals 0.068+_0,0021 2 Dinitrophenol administrated animals 0.035+_0,0054 3 Rotenon administrated animals

Tab 10. Change of contents of deprotonized products in 5 membrane structures,belonging to the fourth compartment (serum TBA-positive products )during modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling ).

Study groups

1 2 3

Control animals Dinitrophenol administrated animals Rotenon administrated animals

The contents of deprotonized products in 5 membrane structures,belonging to the fourth compartment (serum TBA-positive products ) 0.077+_0,0022 0.093+_0,0029 0.056+_0,0038

Tab 11. Change of the resistance of 5 membrane structures,belonging to the fourth compartment during modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha statehigh oxy potential elevated situation in rabbits (after three days of modelling ).

Study groups

1 2 3

Control animals Dinitrophenol administrated animals Rotenon administrated animals

The resistance of 5 membrane structures,belonging to the fourth compartment 0.499+_0,033 0.804+_0,051 0.462+_0,023

Tab 12. Change of the antipereoxidation resistance of 5 membrane structures,belonging to the fourth compartment during modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling). Study groups The antipereoxidation resistance of 5 membrane structures,belonging to the fourth compartment 1.830+_0,093 1 Control animals 2.508+_0,197 2 Dinitrophenol administrated animals 2.155+_0,093 3 Rotenon administrated animals Tab 13. Change of the osmotic the fourth compartment during resistance of 5 membrane structures,belonging to modelling of membrane high betta state-high

reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling). Study groups The osmotic resistance of 5 membrane structures,belonging to the fourth compartment 0.732+_0,013 0.606+_0,037 0.724+_0,113

1 2 3

Control animals Dinitrophenol administrated animals Rotenon administrated animals

Tab 14. Change of contents of cholesterols in the second compartment during modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling). 1 2 3 Study groups Control animals Dinitrophenol administrated animals Rotenon administrated animals The contents of cholesterols in the second compartment 3.34+_0,087 3.193+_0,093 3.70+_0,066

Tab 16. Change of contents of LPHD in the second compartment during modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling ). Study groups The contents of LPHD in the second compartment 1.63+_0,088 1.76+_0,093 1.69+_0,083

1 2 3

Control animals Dinitrophenol administrated animals Rotenon administrated animals

Tab 17. Change of contents of LPLD in the second compartment during modelling of membrane high betta state-high reduction potential elevated situation and during of modelling of membrane high alpha state-high oxy potential elevated situation in rabbits (after three days of modelling ).

 1 2 3

Study groups Control animals Dinitrophenol administrated animals Rotenon administrated animals

The contents of LPLD in the second compartment 2.79+_0,013 3.00+_0,022 2.98+_0,033

Parameters of body weight and body temperature. Days of observation Parameters of observation The body Control animals weight Dinitrophenol administrated animals Rotenon administrated animals The body Control animals temperature Dinitrophenol administrated animals Rotenon administrated animals Discussion. 1.We observed that in the case of modelling of high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process ) are provocated the increase of body temperature ,decrease of reduced form of NADPH contents, increase of oxidized form of NADP contents ,oxidizing activity of hemaglobine molecules , decrease of reduction :oxidation ratio in a first and fourth compartments ,also reduction activity of membrane-redoxy potentials three state line systems,decrease of membrane resistance of membrane structures of 1 compartment ,elevation of quantity of LPHD in second compartment, increase of glucose utilization speed in second compartment with following decrease of glucose concentration in a second compartment, increase of oxidized- deprotonized in a 5 membrane structures,belonging to fourth compartment ,exhibiting a sun like hot,hot oil external characteristics ,coded by abstract mkhris thermin. 2.We established that in the case of modelling of high alpha state-high oxy potential elevated situation in rabbits by dinitrophenol (dinitrophenolmediated proton loss process )are increased the quantity of LPHD in a second compartment , decrease of LPLD,cholesterols,triglycerids in the medium between cell membrane structures-serum,representing a second compartment,paralleled with a high alpha state-high oxy potentials in membrane-redoxy potentials three state line systems, ,exhibiting a sun like hot,hot oil external characteristics ,coded by abstract mkhris thermin. 2 day 3 days 1.687 1.675 1.775 37.43 38.2 37.23 4 days 1.687 1.615 1.837 37.83 38.25 37.13 5 days 1.825 1.60 1.90 37.0 38.4 36.75 6 days 1.86 1.58 1.90 16 days 1.98 1.88 1.97 22 days 1.74 1.68 1.65

1.687 1.675 1.725 37.18 38.6 38.6

37.35 37.68 37.2 38.5 37.07 36.73 36.6 36.6 36.2

3.We observed that in the case of modelling of high solid betta state mediated high reduction potential elevated situation in rabbits by rotenone (by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) are provocated the decrease of body temperature , increase of reduced form of NADPH contents, decrease of oxidized form of NADP contents ,deline of oxidizing activity of hemaglobine molecules , increase of reduction :oxidation ratio in a first and fourth compartments ,elevation of reduction activity of membraneredoxy potentials three state line systems,increase of membrane resistance of membrane structures of 1 compartment ,decline of quantity of LPHD in second compartment, decrease of glucose utilization speed in second compartment with following increase of glucose concentration in a second compartment, decrease of oxidized- deprotonized products in a 5 membrane structures,belonging to fourth compartment ,exhibiting a moon like cold, cool oil external characteristics ,coded by abstract badgan thermin. 4. We observed that in the case of modelling of high solid betta state mediated high reduction potential elevated situation in rabbits by rotenone(by blocking cytochrome oxidase in the complex 4 with elevation of reduced form of NADH ) are decreased the quantity of LPHD in a second compartment , increase of LPLD,cholesterols,triglycerids in the medium between cell membrane structures-serum,representing a second compartment,paralleled with a high solid betta state-high reduction potentials in membrane-redoxy potentials three state line systems, ,exhibiting a moon like cold, cool oil external characteristics ,coded by abstract badgan thermin.

Definition of Mkhris abstract thermin. Abstract Mkhris thermin showed : a. Relatively high level of fluid alpha states,consisting of unsaturated fatty acids with high levels of oxy potentials and with high levels of proton,electrons conductance and high levels heat energy release, middle degree of energy accumulation and middle degree of high energy phosphate-ATP, high energy electrons NADPH, with middle ratio of donators :accceptors are associated with abstract theory of Mkhris,which is distinguished by hot,hot oil,acute external characteristics. b.The change of fourth compartment parameters ,named as 5 membrane structures-5 function systems,ensuring normal genetic-cell division ,information-response ,biosynthetical , bioenergetical ,biotransformation functions by using of high energy phosphate-ATP, high energy electrons NADPH and heat energy,generated in membrane-redoxy potentials three state line systems in the form of unregulated intensification of information-response functions are coded by abstract Mkhris code. c.The Change of fourth compartment parameters ,named as 5 membrane structures-5 function systems,ensuring normal genetic-cell division ,information-response ,biosynthetical , bioenergetical ,biotransformation functions by using of high energy phosphate-ATP, high energy electrons NADPH and heat energy,generated in membrane-redoxy potentials three state

line systems in the form of slowing of information-response functions are coded by abstract Mkhris code in the Tibetian Mongolian Traditional medicine. d. The change of Third compartment parameters in the form of increase of unsaturated class of visceral and external under skin fatty acids, also decrease of donators, increase of acceptors are coded by abstract Mkhris code in the Tibetian Mongolian Traditional medicine. Definition of Badgan abstract thermin. Abstract Badgan thermin showed : a. Relatively high level of solid betta state,consisting of mainly saturated fatty acids, conditioning a high levels of red potentials and with slow levels of proton,electrons conductance and low levels of heat energy release, high degree of energy accumulation and high degree of high energy phosphate-ATP, high energy electrons NADPH, with increased ratio of donators :accceptors are associated with abstract theory of Badgan ,which is distinguished by cool ,cold oil, stupid external characteristics. b.The change of fourth compartment parameters consisting of serum and extracellular system,where donators,acceptors and some metabolites formed in the result of functioning of 5 membrane structures-5 function systems,ensuring normal genetic-cell division ,informationresponse ,biosynthetical , bioenergetical ,biotransformation functions in the form of increase of contents of donators, decrease of acceptors and increase of AO- decrease of SPOL system are coded by abstract Badgan code in the Tibetian Mongolian Traditional medicine. c.The change of fourth compartment parameters consisting of serum and extracellular system,where donators,acceptors and some metabolites formed in the result of functioning of 5 membrane structures-5 function systems,ensuring normal genetic-cell division ,informationresponse ,biosynthetical , bioenergetical ,biotransformation functions in the form of decrease of contents of donators, increase of acceptors and decrease of AO- increase of SPOL system are coded by abstract Badgan code in the Tibetian Mongolian Traditional medicine. d.The change of Third compartment parameters in the form of increase of saturated class of visceral and external under skin fatty acids, also increase of donators, decrease of acceptors are coded by abstract Badgan code in the Tibetian Mongolian Traditional medicine.

Definition of Rlung abstract thermin. Abstract Rlung thermin showed : a. Relatively high level of gamma state,consisting of decreased contents of saturatedunsaturated fatty acids, conditioning a decreased levels of redoxy potentials and with slow levels of proton,electrons conductance and low levels of heat energy release and energy accumulation and low degree of high energy phosphate-ATP, high energy electrons NADPH, with decreased contents of donators :accceptors,increased significance of proton,electrons prior to generation of proton gradients, prevailed slip mecchanism are associated with abstract theory of rlung ,which is distinguished by light,mobile , nonoil, cool external characteristics.

b.The change of fourth compartment parameters, consisting of serum and extracellular system,where donators,acceptors and some metabolites formed in the result of functioning of 5 membrane structures-5 function systems,ensuring normal genetic-cell division ,informationresponse ,biosynthetical, bioenergetical,biotransformation functions in the form of decrease of contents of donators,acceptors and some metabolites formed in the result of functioning of 5 of membrane structures-5 function systems,including synthetic, bioenergy,biotransformation functions are coded by abstract Rlung code in the Tibetian Mongolian Traditional medicine. c.The change of fourth compartment parameters ,named as 5 membrane structures-5 function systems,ensuring normal genetic-cell division ,information-response ,biosynthetical , bioenergetical ,biotransformation functions by using of high energy phosphate-ATP, high energy electrons NADPH and heat energy,generated in membrane-redoxy potentials three state line systems in the form of decrease of synthetic, bioenergy,biotransformation functions and of unregulated disturbance of information-response functions are coded by abstract Rlung code in the Tibetian Mongolian Traditional medicine. d.The change of third compartment parameters in the form of decrease of visceral and external under skin fatty acids, also donators,acceptors are coded by abstract Rlung code in the Tibetian Mongolian Traditional medicine. References: 1. Ambaga M, Sarantsetseg B, Rlung, mkhris, badgan- membrane structures, UB,1995, 108 p. 2. Ambaga M, Sarantsetseg B, Additional lectures on Traditional medicine 2008, 109 p. 3. Ambaga M, Sarantsetseg B, 100 questions and answers of Traditional Medical Studies, UB, 2005, 80 p.. 4. Ambaga M, Sarantsetseg B, Experimental research results,obtaining as main code of new medical system-NCM UB, 2009, 80 p.. 5. Ambaga M, Sarantsetseg B, Explanation of some theoretical questions of Traditional medicine at the level of cells, membranes UB,2002 , 250 p. 6. Ambaga M, Sarantsetseg B, Comparative study of basic theories of Oriental medicine/ similarities and differences UB,1997, 106 p.. 7. Ambaga M, Sarantsetseg B, Bold Sh , Philosophy, cognition and scientific bases of Oriental Medicine UB,1996, 109 p.. 8. Ambaga M, Sarantsetseg B, Code conversion between theoretical aspects of Traditional and Modern medicine UB,2005, 180 p.. 9. Ambaga M, Sarantsetseg B, Theoretical and methodological bases of united diagnosing and therapy chain in Traditional and Modern Medicine,UB,2005, 200 p. of 50 principal decisions in framework of new conception of three membrane-redoxy potentials line systems

10. Ambaga M, Sarantsetseg B, The theoretical aspects of producing and activation of all medicaments through two main conveyer system,including industry and living cells,UB, 2008, p.108. 11. Ambaga M, Sarantsetseg B, Integration of Traditional Medicine and Pharmacy in the frame of conception-two steps of producing and activation of medicinal products UB, 2008, p.180. 12.Bhagwan Dash,Concept of agni in Ayurveda,1971,212 p. 13.Dwarakanath C,Digestion and metabolism in Auyrveda,2003,360 p. 14.Hyun-Ju Kim, Seung Yeon Hwang, Ju-Ho Kim et all, Association between genetic Polymorphism of multidrug resistance 1 gene and Sa sang constitutions, Evidence-Based Complementary and Alternative Medicine,2009,vol.6,S1,p.73-80.15. Micheal Heinrich,Joanne Barnes,Simon Gibbons,Fundamentals of Pharmacognosy and phytotherapy,churchill livingstone.16. Voet D, Biochemistry 1990,1221 p.