PENGARUH EKSTRAK DAUN BELIMBING WULUH (Averrhoa bilimbi L) TERHADAP GAMBARAN HISTOPATOLOGI PULAU LANGERHANS PANKREAS TIKUS PUTIH

(Rattus norvegicus)
Purwamastya Wahyu Hidayat

ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak daun belimbing wuluh (Averrhoa bilimbi L) terhadap gambaran histopatologi pulau Langerhans pankreas tikus putih (Rattus norvegicus). Sebanyak 20 ekor tikus putih (Rattus norvegicus) galur wistar jantan 100-150 gram berumur 2-3 bulan dibagi dalam 5 kelompok P0, P1, P2, P3, dan P4. Kelompok pertama (P0) sebagai kontrol normal hanya diberi NaCl fisiologis, P1 di Induksi aloksan 150 mg/kg BB secara intraperitonial dan diberi CMC Na sebagai kontrol perlakuan , P2 diberi ekstrak belimbing wuluh 307 mg/kg BB, P3 400 mg/kg BB, P4 461 mg/kg BB. Perlakuan dilakukan selama 8 hari, setelah itu tikus dibunuh dan diambil organ pankreasnya untuk dibuat sediaan histopatologi dengan menggunakan pewrnaan Hematoxilyn-Eosin (HE). Rancangan percobaan yang digunakan rancangan acak lengkap dan hasilnya dianalisis menggunakan ANAVA (Analyzed of Varience) dengan menggunakan SPSS versi 20, kemudian jika terdapat perbedaan yang nyata dilanjutkan Uji Jarak berganda duncans.

Key word: aloksan, pulau Langerhans, pankreas, ekstrak belimbing wuluh.

Effect of Leaf Belimbing Wuluh (Averrhoa bilimbi L) Extraction to Description of Pancreatic Islets of Langerhans Histopathology White Rats (Rattus norvegicus)

Purwamastya Wahyu Hidayat

ABSTACT The aims of this study was obtained the effect of leaf belimbing wuluh (Averrhoa bilimbi L) extraction to description pancreatic islets of Langerhans histopathology in white rats (Rattus norvegicus). Twenty white male rats (Rattus norvegicus) wistar strain with 100-150 gram average body weight and two three month old devided into five group P0, P1, P2, P3, and P4. First group (P0) as normal control was given physiological NaCl, P1 which induced by alloksan intraperitoneally and given CMC Na as treatment control. P2 given exstract belimbing wuluh 307 mg/kg b.w, P3 400 mg/kg b.w, P4 461 mg/kg b.w. the treatment were done for 8 days, then rats were killed the pancreas were taken for histology preparation and stained with HE(Hematoxylin Eosin). This study used complete randome design and the result has been analyzed using ANAVA (Analyzed of Varience) continued with Duncans Multiple Range Test Method and processed by using SPSS version 20.

Key word: alloxan, islets of Langerhans, pancreas, extract belimbing wuluh