P3: BIOTECHNOLGY BIOTECHNOLOGY / SEM-IV / SEM-IV / SIDDY / SIDDY UNIT-I: PROTEIN ENGINEERING

Immobilized enzymes & Cells. Bioreactors Designs. Methods of Immobilization & its Applications. Resolution of amino acid Recemates. Synthesis of Improved pencillins. Increased Protein Stability. Enhanced Specific activity & multiple activation. Altering the kinetic properties, thermal stability & pH dependence of enzymes. Macro modifications. Use of poly arginine tail in protein purification. Themostable Enzymes. Abzymes. Biosensors: principle & applications. Introduction to drug design & delivery.

UNIT- II: MICROBIAL BIOTECHNOLOGY Introduction to Microbial Biotechnology.

Large scale cultivation of microbes, problem of oxygen supply. Basic fermentor design of stirred tank Reactor. Aseptic operation. Control systems. Batch vs continuous operation. Down stream processing. Production of Biomass(microbial insecticides, starter cultures, Single cell protein productions) Primary & secondary metabolites(riboflavin, vit B12,glutamic acid, ethanol, penicillin & streptomycin. Metabolic End products.

Production of low molecular compounds:

Bioconversions. Microbial polysaccharides. Microbial enzymes (Invertase, Amylase, cellulases. Lipases & Proteases). Microbiological mining

 Bioremediation of Toxic Chemicals. Biofertilizers (Nitrogen phosphorous fixing microbes. Micorrhiza (endo & ecto). Principles of biological control of Disease. UNIT-III: ANIMAL BIOTECHNOLOGY
Introduction to animal biotechnology. Cells & cell lines. Media for cell structure & equipment isolation of different types of cells from tissue & their short term & long term culturing. Characteristics of normal & transformed cells. Production of viral vaccines. Production of high value therapeutics interferon & plasminogen activator. Urokinase .monoclonal antibodies. Immunotoxins as Therapeutic agents. Chimeric Abs. Introduction to transgenic animals. Human gene therapy. Animal cloning techniques. Gene Knockouts. Introduction to genomics & proteomics. Adult & embryonic stem cells : Principles of cell therapy & its applications in Medicine.

UNIT-IV: PLANT BIOTECHNOLOGY

INTRODUCTION TO PLANT BIOTECNOLOGY. Plant cell culture media & techniques. Plant protoplasts & protoplast fusion. Plant viruses as vectors. Ti plasmid as vectors. Principles of generation of transgenic plants. Applications of transgenic technology in agriculture. Anti sense RNA & DNA. Genetically modified foods & its legalization. Finger printing of plant extracts for therapeutic potency. Edible vaccines.