Clinical Nutrition Supplements (2012) 7(S1), 25249

Poster presentations
Carbohydrate and lipid metabolism
PP001-SUN Outstanding abstract EFFECT OF ACUTE ENERGY DEFICIT AND ENERGY SURPLUS ON VLDL-TG METABOLISM IN WOMEN E. Bellou1 , H. Botonaki1 , M. Maraki1 , F. Magkos1,2 , S.A. Kavouras1 , L.S. Sidossis1,3 . 1 Nutrition-Dietetics, Harokopio University, Kallithea Athens, Greece; 2 Internal Medicine, Center for Human Nutrition and Atkins Center of Excellence in Obesity Medicine, Washington University School of Medicine, St. Louis, MO, 3 Internal Medicine, Sealy Center on Aging, Institute for Translational Sciences and Shriners Hospital for Children, University of Texas Medical Branch at Galveston, Texas, United States Rationale: Acute dietary energy restriction (>500 kcal) decreases very low-density lipoprotein triglyceride concentration (VLDL-TG). However, the effect of acute overfeeding on VLDL-TG concentration is not known. Furthermore, the mechanisms regulating the response of VLDL-TG to acute changes in energy intake have not been studied in women. The aim of the present study was to investigate the effects of negative and positive energy balance on VLDL-TG metabolism. Methods: Ten healthy women (age: 22.02.9 years, BMI: 21.21.3 kg/m2 ) underwent a stable isotopically labeled tracer infusion study after performing, in random order, three experimental trials on the previous day: i) hypocaloric feeding (dietary energy restriction of 700 kcal), ii) hyper-caloric feeding (overfeeding of 700 kcal), and iii) a control day of isocaloric feeding (net energy balance). All diets had the same macronutrient content (%). Subjects were instructed to replicate their diet for two days before each trial and to avoid any kind of physical activity. Results: Fasting plasma VLDL-TG concentrations were ~25% lower after diet compared to control (P =0.037). There was no signicant change on VLDL-TG concentration after overfeeding compared to control. Hepatic VLDL-TG secretion rate was reduced by ~21% after diet (P =0.041) but was not different after overfeeding (P =0.741), whereas the plasma clearance rate of VLDL-TG was increased by 12% after diet (P =0.016) but not after overfeeding (P =1), compared to control. Conclusion: Energy decit elicited by calorie restriction leads to hypotriglyceridemia via decreased hepatic VLDL-TG secretion and increased VLDL-TG clearance. This effect is different from the one observed after exerciseinduced-energy decit, where there is an increase in VLDL-TG clearance but no change in VLDL-TG secretion. A single day of overfeeding (plus 700 kcal) was not sufcient to change basal VLDL-TG kinetics in healthy women.
Disclosure of Interest: None Declared.

PP002-SUN SERUM CHOLESTEROL LEVELS AND AMYOTROPHIC LATERAL SCLEROSIS (ALS) SURVIVAL: RETROSPECTIVE SURVEY OF 103 PATIENTS V. Herrera1 , G. Creus1 , N. Virgili1 , C. La Torre2 , O. Pardina2 , M. Povedano2 , C. Flores2 . 1 Endocrinology and Clinical Nutrition Department, 2 Neuromuscular and Neurology Department, Hospital Universitario de Bellvitge, Barcelona, Spain Rationale: Amyotrophic lateral sclerosis (ALS) is a degenerative disease of unknown aetiology. Besides age and site of onset, no other factors have been consistently demonstrated to be related to ALS outcome. Recently, hyperlipidemia has been reported to be related to a better prognosis with an increase of survival but there are controversies in different published works. The aim of the study is to analyze the relationship between serum cholesterol levels and survival in ALS patients. Methods: Retrospective analysis of 103 deceased patients with ALS diagnosis dened according to El Escorial criteria. Serum cholesterol levels were determined after the beginning of the disease and without considering the evolutionary moment. Other factors like delay for the diagnosis, age at diagnosis, site of onset and rilutek treatment also were evaluated. Furthermore, other criteria have been introduced to determine the nutritional state at the moment of blood analysis. Results: Among 103 ALS patients, 63 (61%) had high serum cholesterol levels. All of them had normal nutritional status with a normal serum albumin levels. No relation was observed between serum cholesterol levels and survival through statistical analysis, but a signicant correlation was found with age, site of onset and delay for the diagnosis. In this group of patients, no relation was observed between rilutek treatment and survival. Conclusion: In our group of patients serum cholesterol levels are not associated with survival. The analysis corroborates the already known relation of favourable prognostic indicators. It would be necessary to realize