Natural Convection Laboratory Incubators Ideal for incubating of micro-organisms, and microbiological heating and conditioning Binder Natural

Convection Laboratory Incubators

Capacities from 20 - 720 liters Temperatures from ambient +5 to 100C Controller features integrated timer settable from 0 to hours and one ad!ustable temperature ramp "tainless steel interior and coated steel e#terior construction

Carbolite Natural Convection Laboratory Incubators

Capacities from 27 - 215 litres Temperatures from ambient +10 to $0C Incubator features a three segment %I& microprocessor controller 'ith an ad!ustable single ramp rate to set point ()ailable 'ith a )ariet* of alternate control options +p to 15 shel)es positions a)ailable

Forced Convection Laboratory Incubators Ideal for applications 'ith high specimen )olumes, such as sample bags and fre,uent batch changes -orced models features a high-performance fan to circulate air 'ithin the chamber to ma#imi.e temperature uniformit* and impro)e reco)er* times Binder Forced Convection Laboratory Incubators

Capacities from 5/ - 720 litres Temperatures from +5 to 100C "peciall* designed air turbines 'ith ad!ustable airflo' rates -ast reco)ering times - 1 to 2 minutes at /7C Controller timer 'ith dela*ed on, dela*ed off and temperature dependent off functions

Carbolite Forced Convection Laboratory Incubators

Capacities from 2$ - 2/0 litres Temperatures from ambient +10 to $0C &esigned for long term accurac* and fle#ibilit* (ccurac* of 05C

Refrigerated Convection Laboratory Incubators %recision incubation control for maintaining specimen ,ualit* at lo' temperatures Binder Refrigerated Laboratory Incubators

Capacities from 20- 720 litres Temperatures from 10C to 100C 1apid temperature reco)er* rates2 under t'o minutes at /7C Controller features dela*ed on, dela*ed off and temperature dependent dela*ed off timer functions plus storage capabilit* up to 20 segments 3ermeticall* sealed inner glass doors to allo' *ou to monitor samples 'ithout influencing chamber conditions

Carbolite Refrigerated Laboratory Incubators

Capacities from /0 - 200 litres Temperatures from 0 to 40C 5odels cooled b* a hermeticall* sealed refrigeration s*stem Internal heatproof toughened glass door allo's clear )ie' of inner chamber 'ithout disturbing the atmosphere 3igh performance fan ensures optimum temperature uniformit*

CO2 Laboratory Incubators -or professionals 'ho need to simulate natural cellular gro'th en)ironment Binder Air-Jacketed CO2 Incubators

Capacities of 150 litres and 210 litres Temperature range of ambient +7C to 100C %recise temperature and C62 control eliminates harmful o)ershoot "eamless stainless steel interior minimi.es

contamination ris7 +nits features intelligent electronic control of temperature and infrared C62 measurement (ir !ac7et s*stem ensures temperature accurac* and repeatable results

In biology, an incubator is a device used to grow and maintain microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity and other conditions such as the carbon dioxide(CO2) and oxygen content o the atmosphere inside. Incubators are essential or a lot o experimental wor! in cell biology, microbiology and molecular biology and are used to culture both bacterial as well as eu!aryotic cells. Incubators are also used in the poultry industry to act as a substitute or hens. This o ten results in higher hatch rates due to the ability to control both temperature and humidity. "arious brands o incubators are commercially available to breeders. The simplest incubators are insulated boxes with an ad#ustable heater, typically going up to $% to $& 'C (()% to (&% '*), though some can go slightly higher (generally to no more than (%% 'C). The most commonly used temperature both or bacteria such as the re+uently used ,. Coli as well as or mammalian cells is approximately -. 'C, as these organisms grow well under such conditions. *or other organisms used in biological experiments,

such as the budding yeast /accharomyces cerevisiae, a growth temperature o -% 'C is optimal. 0ore elaborate incubators can also include the ability to lower the temperature (via re rigeration), or the ability to control humidity or CO2 levels. This is important in the cultivation o mammalian cells, where the relative humidity is typically 12&3 and a slightly acidic p4 is achieved by maintaining a CO2 level o &3. 0ost incubators include a timer5 some can also be programmed to cycle through di erent temperatures, humidity levels, etc. Incubators can vary in si6e rom tabletop to units the si6e o small rooms. There are many other ways to create an incubator. The renowned scientist 7ouis 8asteur used the small opening underneath his staircase as an incubator. The maximum temperature or sterili6ation is 2%%oC . Contents

( Incubator
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(.( 4umid CO2 Incubator

Incubator

I a hot room is not available, it may be necessary to buy an e+uivalent dry incubator. ,ven with a hot room, it is sometimes convenient to have another incubator close to the hood or trypsini6ation. The incubator should be resistant to corrosion (e.g., stainless steel, although anodi6ed aluminum is acceptable or a dry incubator) and easily cleaned. 9 double chamber, or two incubators stac!ed, one above the other, independently regulated, is pre erable to one large incubator because it can accommodate more cultures with better temperature control, and i one hal ails or needs to be cleaned, the other can still be used. 0any incubators have a heated water #ac!et to distribute heat evenly around the cabinet, thus avoiding the ormation o cold spots. These incubators also hold their temperature longer in the event o a heater ailure or cut in power. 4owever, new high:e iciency insulation and di use sur ace heater elements have all but eliminated the need or a water #ac!et and ma!e moving the incubator much simpler. (9 water #ac!et generally needs to be emptied i the incubator is to be moved.) Incubator shelving is usually per orated to acilitate the circulation o air. 4owever, the per orations can lead to irregularities in cell distribution in monolayer cultures, with variations in cell density ollowing the pattern o spacing on the shelves. The variations may be due to convection currents generated over points o contact relative to

holes in the shel , or they may be related to areas that cool down more +uic!ly when the door is opened. 9lthough no problem may arise in routine maintenance, las!s and dishes should be placed on an insulated tile or metal tray in experiments in which uni orm density is important. Humid CO2 Incubator 9lthough cultures can be incubated in sealed las!s in a regular dry incubator or a hot room, some vessels, e.g., 8etri dishes or multiwell plates, re+uire a controlled atmosphere with high humidity and elevated CO2 tension. The cheapest way o controlling the gas phase is to place the cultures in a plastic box, or chamber (;ellco, 08 ;iomedicals)< =as the container with the correct CO2 mixture and then seal it. I the container is not completely illed with dishes, include an open dish o water to increase the humidity inside the chamber. CO2 incubators are more expensive, but their ease o use and superior control o CO2 tension and temperature (anaerobic #ars and desiccators ta!e longer to warm up) #usti y the expenditure. 9 controlled atmosphere is achieved by using a humidi ying tray (*ig &.($) and controlling the CO2 tension with a CO2:monitoring device, which draws air rom the incubator into a sample chamber, determines the concentration o CO2, and in#ects pure CO2 into the incubator to ma!e up any

de iciency. 9ir is circulated around the incubator by natural convection or by using a an to !eep both the CO2 level and the temperature uni orm. It is claimed that an:circulated incubators recover aster a ter opening, although natural convection incubators can still have a +uic! recovery and greatly reduce the ris!s o contamination. >ry, heated wall incubators also encourage less ungal contamination on the walls, as the walls tend to remain dry, even at high relative humidity. /ome CO2 controllers need to be calibrated every ew months, but the use o gold wire or in rared detectors minimi6es dri t and many models reset the 6ero o the CO2 detector automatically. The si6e o incubator re+uired will depend on usage, both the numbers o people using it and the types o cultures. *ive people using only microtitration plates could have (%%% plates ((%%,%%% individual cultures) or (% experiments each in a modest:si6ed incubator, while one person doing cell cloning could ill one shel with one or two experiments. *las! cultures, especially large las!s, are not an economical use o CO2 incubators. They are better incubated in a regular incubator or hot room. I CO2 is re+uired, las!s can be gassed rom a cylinder or CO2 supply.