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EFFECT OF HYPERURICEMIA HERBS FORMULA ON THE FUNCTIONS OF
LIVER
Agus Triyono and Peristian Rid!a
Medicinal Plants and Traditional Medicine esearch and De!elopment "enter# Tawangmangu
$alan %awu &o '' Tawangmangu Karanganyar "entral $a!a (agustriyono)*'+yahoo.com,
ABSTRACT
-yperuricemia herbs formula can be used in formal health ser!ices if pro!en safe and
effecti!e. Pre!ious clinical trials showed that the formula decreased blood uric acid le!els.
.afety of the formula on the functions of the li!er need to be in!estigated before it is
recommended. The study aims to find out the safety information of the formula herbs on
functions of li!er. The formula consisted of se!eral herbs# i.e. Stelechocarpus burahol,
Sonchus arvensis, Caesalpinia sappan, Curcuma xantorriza, C. domestica and Phillanthus
niruri. This formula has been tasted safe from the preclinical tests. "linical trial of the
formula was conducted by using the pre / post design research in!ol!ing 01 subjects who
ha!e met the inclusion and e2clusion criteria. The protocol was appro!ed by the research
ethics of the 3thics "ommittee for -ealth esearch# -ealth esearch and De!elopment
"enter $akarta. The pre/inter!ention was performed anamnesis (a preliminary case historyof
a medical or psychiatric patient, of the subject4s identity# medical history# clinical symptoms#
physical e2amination diagnostic# laboratory tests of li!er function (.56T and .5PT,. 3!ery
week during 7 weeks# the subjects were gi!en the herbal formula and once a week their li!er
functions were controlled. 6n the first day (-',# the subjects were drinking the prepared
boiled formula simplisia# then they gi!en the protocol to prepare it for use at home. 3!ery
day they need to drink the formula three times. esearch subject was control once a week to
do anamnesis complaint# the possibility of side effects and performed a physical e2amination
diagnostic. The li!er functions (.56T and .5PT, of the subjects were checked e!ery
month. The study showed that the anamsesis and physical e2amination of the subject during
inter!ention and after inter!ention did not cause significant side effects. The analysis of
paired test showed there was no significant difference le!els on the .56T (t 8 '.97# p 8
1.'*1 (p:1.19,, and .5PT (t 8 1.;<# p 8 1.9<< (p:1#19,, before and after the inter!ention of
hyperuricemia herbs formula both at day *7th and 9;
th
days. The use of herbs hyperuricemia
herbs formulafor for 9; days did not interfere with li!er function and found no symptoms of
serious side effects. Therefore# the formula is safe for li!er if used in health ser!ices.
"eyords# C$ini%a$ tria$s& !y'eruri%e(ia !er)s *or(u$a& $i+er *un%tion
INTRO,UCTION
=ric acid is the final waste of the metabolism product of purines. =ric acid is actually an
antio2idants in humans and animals# but when its amount is e2cessi!e or reaching saturated in the
blood they e2perience an efflorescence which lead to gout. The process of uric acid metabolism stars
from the chemical process of food containing protein and cellulose that will generate energy. If the
process is de!iated# uric acid will be a build up and called hiperuricemia. (.oenarta and >rieska#
*119,.
-yperuricemia is the condition when uric acid le!els in the blood is not normal i.e.: ? mg@dl
in men and : ; mg@dl in women. -yperuricemia is a metabolic disorder that reAuired long/term
inter!ention. -yperuricemia can be caused by abnormalities of enByme and changes in lifestyle of
eating foods that contain high le!els of purines. Increase le!els of uric acid in the blood will
e!entually end up with the accumulation of monosodium urate in soft tissues especially soft joints.
-yperuricemia can lead to complications such as gout arthritis# uric acid stones# and urate
nephropathy. (-orrison# *11',
-yperuricemia is a problem of metabolic disease that reAuires a long/term therapy or long
life inter!ention. Patient of hyperuricemia and treated with con!entional inter!ention suffer from
bored and looking for alternati!e treatments such as herbal formula. (-idayat# *1'',
%i!er is the largest gland of the bodyC '111/'911 g in weight. It consists of two main lobes#
right lobe and left lobe. %i!er has many functions and comple2. Its function is di!ided into four#
namely formation and e2cretion of bile function# metabolic function# immune function and li!er
!ascular function. Because of its strategic location and its function is comple2# function of the li!er is
easily getting disorders# such as !iral hepatitis# hepatitis due to herbal medicine# drug or to2ic
foodstuffs# li!er cirrhosis# fatty li!er and li!er tumors. (Mc5il!ery and 5olstein# 'DD;,
.ymptoms of li!er disease !aries from asymptomatic to se!ereC li!er disease usually
associated with enByme abnormalities. %i!er produce many enBymes# such as lactate dehydrogenase#
serum transaminase (.56T# .5PT,# gamma glutamyl transpeptidase (5amma 5T,# and alkaline
phosphatase (>%P,. Ehen le!er is damage# enByme produced will be e2cessi!e and e2cretes into the
e2tracellular space. (Mc5il!ery and 5olstein# 'DD;,.
>n herbal formula for treatment of hyperuric acid such as .56T and .5PT has been tested
and it is safefor lowering uric acid in the blood.
The study was aimed to assess the effect of hyperuricemia herbs formula for li!er function
(.56T and .5PT,.
MATERIALS AN, METHO,S
.implicia raw materials consisted of Stelechocarpus burahol %, Sonchus arvensis %,
Caesalpinia sappan %, Curcuma xantorriza, Curcuma domestica Val, Phillanthus niruri %. were taken
from the Tawangmangu and Karangpandan. The plant materials were washed with tap water to
remo!e debris# then aerated in acontinued drying o!en at 91
1
" for ? hours. The dried plants were
packaged in a plastic bags.
Pre'aration o* !er)a$ drin-
.tarting the first day of the study# subjects were gi!en the herb botanicals (dried preparation,
blood uric acid/lowering formula# which has been packed and accompanied by rules boil and drink
herbs (one package boiled with 9 cups (*11 cc, of water to a boil so that < cups of water to drink in
the morning# noon and afternoon,# one package for one day# the ne2t day boil the new packaging.
-erbs taken continuously for 9; days.
This research is a Auasi e2perimental pre and post test design conducted in .aintification
-erbal "linic. .tudy in!ol!ed 01 research subjects who ha!e met the inclusion and e2clusion criteria.
Inclusion criteria wereF age *1/;9 years old# men or women# subjects with blood uric acid le!els ?/'1
mg @ d%# willing to follow study @ schedule follow-up by signing informed consent. 32clusion criteria
wereF pregnant or lactating women (based recognition,# subjects taking medications that affect disease
were obser!ed# subject with se!ere disease complications (eg ad!anced cancer # etc,# subjects had
other comorbidities that affect the clinical condition.
esearch subjects who had signed informed consent were performed anamnesis subject4s
identity# medical record# clinical symptoms# diagnostic physical e2amination# laboratory tests of li!er
function# kidney function# and blood routine. esearch subjects were gi!en the test material in a
number of studies to use for se!en days# then once a week they were controlled up to confirm that
they ha!e taken the test material as reAuired. 6n the first day (-',# subjects were gi!en the boiling
concoction hyperuricemia drink three times a day for 9; days. The subjects were controlled once a
week for a complaint history# de!elopment of clinical symptoms# and possibility of side effects by
physical e2aminations# followed with the e2amination of .56T and .5PT conducted e!ery four
weeks.
This study has recei!ed an ethical clearance from the 3thics "ommittee of &ational Institute
of -ealth esearch and De!elopment# Ministry of -ealth $akarta.
RESULTS AN, ,ISCUSSION
To assess the effect of hyperuricemia herbs formula on the functions of the li!er# there has
been carried out laboratory tests .56T and .5PT for 01 subjects at three different times# before the
inter!ention# the *7th day and the 9;th day after the inter!ention. The mean le!el of .56T and .5PT
study subjects are shown in Table '.

Ta)$e./0 Mean $e+e$ o* S1OT and S1PT o* t!e 'atients )e*ore and treated it! !y'eruri%e(iua
!er)s *or(u$a
Li+er
*un%tion
Be*ore
Inter+ention
,ay.23 ,ay.45
.56T *?.D< *'.D' *1.?0
.5PT *'.90 *1.?' *1.?;

To determine the effect of hyperuricemia herbs formula on li!er function# it is conducted to
analyBe the differences in le!els of .56T and .5PT before and after *7 days inter!ention# with a
paired t test. The analysis results are shown in Table *.

Ta)$e.20 Ana$ysis o* $e+e$s o* S1OT and S1PT o* t!e 'atients )e*ore and a*ter treated it!
!y'eruri%e(iua !er)s *or(u$a
Li+er
*un%tion
Be*ore inter+ention 6H78
A*ter inter+ention
6H238
t '
MeanG S, meanG S,
.56T *;.?' '?.;1 **.?' '1.7; '.97 1.'*1
.5PT *7.0? '?.19 *?.1; '0.DD 1.;< 1.9<<
9Nu()er o* treated sa('$es ere :7
Table * shows that there is no significant difference in le!els of .56T (t 8 '.97# p 8 1.'*1
(p: 1.19,, and .5PT (t 8 1.;<# p 8 1.9<< (p: 1.19, , before and after inter!ention of hyperuricemia
herbs formula day *7.
To determine the effect of hyperuricemia herbs formula on li!er function# to analyBe
differences in le!els of .56T and .5PT before and after 9; days# with a paired t test. The analysis
results are shown in Table <.
Ta)$e ;0 Ana$ysis o* $e+e$s o* S1OT and S1PT ,i**eren%es )e*ore and a*ter treated eit! t!e
!er)a$ *or(u$a
Li+er
*un%tion
Be*ore inter+ention 6H78
A*ter inter+ention
6H458
t '
mean S, mean S,
.56T *;.'D '?.;* *0.<0 ''.01 1.?7 1.0<D
.5PT *?.7? ';.77 *D.17 *0.99 1.<? 1.?'?

Table <. shows that there is no significant difference in le!els of .56T (t 8 1.?7# p 8 1.0<D
(p: 1.19,, and .5PT (t 8 1.<?# p 8 1.?'? (: 1.19,, before and after inter!ention hyperuricemia herbs
formula day 9;.
3nByme of .56T (serum glutamic o2aloacetic transminase, and .5PT (serum glutamic
pyru!ic transaminase, is a serum transaminase sensiti!e to damage li!er cells. Increase two times or
more than the normal le!el of .56T and .5PT enByme is diagnostic of impaired li!er cells. The
increase of these enBymes could be due to damage to the li!er cells by herbs or drugs are to2ic to the
cells of the li!er (hepatoto2ic,. These analysis results obtained .56T and .5PT before and after
inter!ention inter!ention of herbs hyperuricemia were not significantly different# means the
inter!ention of herbs hyperuricemia for 9; days did not interfere with li!er function. (Mc.5il!ery and
5olstein# 'DD;,
CONCLUSION
The use of hyperuricemia herbs formula for 9; days did not interfere with li!er function#
therefore the formula safe for li!er# if used in health ser!ices.

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