CLSC 4440

Objectives
Steroid Hormones
NOTE- Also remember the general objectives stated in the syllabs
A!ter com"leting the reading assignment and "artici"ating in class discssion# a stdent $ill be able to do the
!ollo$ing $ith at least %0& accracy'
Corticosteroids
1. List or recognize the structural features which are characteristic of the corticosteroids.
2. Recognize the structure of: cortisol and aldosterone
3. Describe the mechanism of action utilized by the corticosteroids
4. Classify corticosteroids as glucocorticoids or mineralcorticoids and discuss their biological effects.
. Describe the relationshi! between synthesis of corticosteroids and !resence of the following enzymes:
11"#hydro$ylase and 21"#hydro$ylase.
%. Describe biosynthesis of corticosteroids and interaction of angiotensin && and 'C() in this !rocess.
*. Recognize !regnenolone+ 1*#,) !rogesterone+ and !rogesterone as intermediates in biosynthesis of
corticosteroids.
-. Describe the trans!ort of cortisol and aldosterone in the !lasma.
.. Describe the structure+ site of synthesis+ and regulation of 'C() /corticotro!hin0
11. Describe factors which regulate the synthesis and !lasma le2els of glucocorticoids.
11. Describe factors which regulate the synthesis and !lasma le2els of mineralcorticoids 3aldosterone4.
12. Describe diurnal 2ariation of cortisol le2els.
13. Describe metabolism and e$cretion of corticosteroids.
14. Describe !ro!er s!ecimen collection and handling for !lasma cortisol to include determination5effect of
diurnal 2ariation.
1 Discuss !rinci!le of immunological methods for measuring !lasma cortisol to include function of '67 or
sodium salicylate in assay mi$ture.
1%. Discuss measurement of 8urinary free cortisol8+ relate this 2alue to !lasma cortisol le2els+ and recognize
clinical significance of this 2alue..
1*. Recognize reference inter2als for !lasma cortisol /'9 and :90.
1-. Discuss s!ecimen collection and handling for aldosterone assay.
1.. Describe s!ecimen re;uirements for !lasma 'C() le2els.
21. Discuss clinical usefulness of measuring !lasma 'C() and inter!ret results.
1
21. Describe !rinci!le and inter!ret results of following tests:
a. meta!yrone b. 'C() stimulation test
c. De$amethasone su!!ression test
/D97(0 d. Lo
dose D97(
22. Recognize the following sym!toms associated with Cushing<s syndrome:
=(( :lasma cortisol Lac> of circadian rhythm
,besity 'C() changes in le2els of 6a ? @
23 Describe a!!ro!riate screening and confirmatory tests and recognize clinical sym!toms and laboratory
findings associated with d$ of CushingAs syndrome.
Re2ised 2111
24. Recognize maBor causes of !rimary ? secondary aldosteronism+ a!!ro!riate laboratory tests for
diagnosis+ and inter!ret results.
2 Recognize the following as associated with androgenital syndrome and reason for these sym!toms.
a. lac> of hydro$ylase enzymes d. electrolyte imbalance
b. e$cretion of androgens e. hy!ertension
c. male secondary se$ characteristics
2%. Relate !lasma corticosteroid le2el to the following diseases:
CushingAs syndrome 'drenal hy!er!lasia
'ddison<s disease 'ndrogenital syndrome )y!o!ituitarism
2*. Differentiate between diseases abo2e /C2%0 gi2en results of:
a. :lasma cortisol 3'9+ :94 c. 9eta!yrone test e. 'C()
stimulation test
b. :lasma 'C() d. De$amethasone su!!ression test
2-. Recognize clinical sym!toms associated with+ a!!ro!riate laboratory testing+ and discuss !ossible causes
of !rimary adrenal insufficiency /'ddison<s Disease0
31. Recognize laboratory findings associated with 'ddison<s disease
31. =i2en a set of !atient test results+ identify the ty!e of adrenal disorder !resent. Dustify your choice.
(ev )0**
Old Com"ter+,y -ocments+clsc 4440+steroid hormones+steroid hormones obj)0**
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