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Singlepath

Salmonella
For the rapid detection of Salmonella in food
Singlepath

Salmonella
Reliable As sensitive as the official culture media method.
Provides accurate results:
Sensitivity 100 % (milk), specificity 100 % (milk).
Fast Result in just 20 minutes.
Ease-of-use One-step format avoids working errors during handling.
Convenient Simply add sample and read off the result.
Safe Clear and distinct positive or negative test
results with a built-in positive control.
Economical Rapid results save labour and inventory costs and
reduce labour-intensive plating methods. No capital
investment required for example for instrumentation
such as automated systems.
Your benefits
Salmonella spp. is one of the most common causes of
food poisoning worldwide. With an estimated volume of
32 million tests a year, it is probably the food pathogen
most frequently tested for. Salmonella spp. have been
isolated from most types of raw food (meats, eggs, as well
as plant products), and their high resistance to drying
combined with a very high heat resistance once dried,
makes Salmonella a potential problem in most foods,
particularly in dry and semidry products.
Currently, the most commonly used techniques to test for
Salmonella in food products are traditional methods
based on culture media. Reference methods for the
isolation and identification of Salmonella are published
by national (AFNOR, AOAC, DIN, FDA-BAM, USDA, etc.)
and international (ISO) committees and organisations.
Screening for the presence of Salmonella in food
products by conventional reference methods is time-con-
suming and labour-intensive. It usually involves a
3-step technique: non-selective pre-enrichment (18-24h),
selective enrichment in two different selective broth
media (24-48h) followed by plating on two different
selective/indicative agars (24-48h). The whole procedure
takes a total of up to 5 days to get a simple yes/no result.
For products where a positive release system is import-
ant, this means a delay of 5 days before those products can
be released into the market.
The requirement of food manufacturers for quicker re-
lease of finished products and for cost savings are calling
for a change in these methods. Rapid methods, especially
highly convenient tests like immunochromatographical
one-step devices are therefore increasingly interesting
to food manufacturers and distributors. The general
expectation for a rapid test is to be sufficiently sensitive
and specific, user friendly and cost effective. Although
DNA probes are more specific than immunological tests,
they do not always fulfill other relevant user criteria.
Immunological tests are therefore often the preferred
choice among users of rapid tests.
The new Singlepath Salmonella immunochromato-
graphical test offers all these benefits and makes testing
for Salmonella fast, reliable and convenient.
Flow-diagram of Singlepath

GLISA Salmonella test procedure

Peptone Water (Buffered) (BPW) 500g 1.07228.0500
Peptone Water (Buffered) (BPW) 5kg 1.07228.5000
Salmonella enrichment broth 500g 1.07700.0500
Singlepath Salmonella 25 tests 1.04140.0001
Rambach agar for 1l 1.07500.0001
Rambach agar for 4l 1.07500.0002
XLD agar 500g 1.05287.0500
XLD agar 5kg 1.05287.5000
XLT4 agar 500g 1.13919.0500
Product list
Products Pack size Ord.No.
Enrichment
Detection
Isolation media
acc. to Rappaport-Vassiliadis (RVS broth)
Transfer 160 l to test device
and read result after
20 min.
Salmonella
not present
Salmonella
present
negative
positive
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Inoculate 0.1 ml into 10ml RVS broth
Incubate at 41.5C for 24 3h
Tansfer 1-2ml to an appropriate tube
Cover with loose fitting cap
Heat in boiling water bath 15min.
Cool to room temperature
Sample 25g/ml in 225ml BPW
Incubate at 37C for 182h
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n If positive result:
Streak onto differential agars
for confirmation
e.g. Rambach agar, XLT agar,
XLD agar
Same safety standard as the classical detection method:
Simple to perform, reliable results in just 20 minutes, considerable
savings in time and costs.
Wider product range:
Lateral flow tests detect important pathogens in food and environment:
E.coli 0157, Verotoxin-producing E.coli, Salmonella, Campylobacter, Listeria,
Listeria monocytogenes, Legionella and enterotoxinogenic Bacillus cereus.
Additional plus:
Especially adapted media for precise and reliable results.
Lateral flow tests
For the rapid detection of
pathogens in food
Merck KGaA
64271 Darmstadt, Germany
Fax: +49 (0) 6151/72 60 80
Email: microbiology@merck.de
www.merck.de
microbiology.merck.de
We provide information and advice to our customers to the best of our knowledge and ability, but without obligation or liability. Existing laws and
regulations are to be observed in all cases by our customers. This also applies in respect to any rights of third parties. Our information and advice do not
relieve our customers of their own responsibility for checking the suitability of our products for the envisaged purpose.
Singlepath is a registered trademark of Merck KGaA, Darmstadt, Germany.
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