XAC NH NHU CAU OXY HOA HOC

TRONG NC VA NC THAI
SMEWW 5220 C - 2005
A. GII THIEU
1. Nguyen tac
Hau het

cac chat hu c b oxy hoa bi hon hp Kali

dichromate va axit Sunfuric nong. Mau c phan huy bi dung

dch axit manh va lng d biet trc Kali dicromat (K2Cr2O7). Sau
khi phan huy, lng K2Cr2O7 d c chuan o bang dung dch
Sunfat amoni sat (II) (FAS). Lng K2Cr2O7 dung e phan huy
chat hu c c tnh dang oxy tng ng.

2. Cac yeu to anh hng

He phan huy phi kn e gi c cac chat hu c de
bay hi khi phan huy.
Kiem tra nap ong phan ng.
Chon kch c ong phan ng phu hp.
Cac hp chat beo mach thang c oxy hoa hieu qua
hn khi co mat chat xuc tac nh Ag 2SO4. Lng Ag2SO4 se
giam khi co mat Cl-, Br-, I-. Loai tr cac halogenua bang
cach cho HgSO4 (1g HgSO4 dung cho 50ml mau vi ieu
kien nong o Cl- nho hn 2000mg/L).
Ham lng NO2- trong mau thng nho nen anh hng
khong ang ke. Neu ln hn 2mg N-NO 2- /L loai tr bang
cach cho 10 mg axit Sulfamic ng vi 1 mg N-NO 2- co mat.

3. Pham vi ap dung
Dung e xac nh nhu cau oxy hoa hoc trong nc va
nc thai

4. Tai lieu tham khao

Standard Methods for The Examination of Water and Wastewater 21 st
Edition 2005

B. THIET B VA DUNG CU
1. Thiet b
Bep nung chuyen dung cho xac nh COD, chnh nhiet o
1500C 50C.

2.

Dung cu

Ong nghiem chu nhiet co nut van bang TFE.

Pipet thang: 5ml, 2ml
Pipet bau : 2ml
Pipet ban t ong: 2-10ml
Buret: 50ml
Becher: 100ml
Erlen: 100ml
Bnh nh mc: 50ml, 100ml, 1000ml

C. HOA CHAT DUNG DCH

1. Hoa chat

K2Cr2O7 (o tinh khiet 0.1N 0.01%)

Ag2SO4

HgSO4

1,10 phenantroline

Fe(NH4)2(SO4)2. 6H2O

FeSO4. 7H2O

H2SO4 (d= 1.84 g/ml)

2. Dung dch
2.1. Kali dicromat 0.1 N: Hoa tan 4.903g K2Cr2O7 (a say 1050C
trong 2 gi) hoac hoa tan hoan toan lng K 2Cr2O7 trong ong
chuan( trang ky nhieu lan bang nc cat ) vao 500mL nc

cat; them 167mL H2SO4 (d=1.84g/mL) va 33.3g HgSO4. Khuay tan,

e nguoi en nhiet o phong. Chuyen qua bnh nh mc
1000ml. Them nc cho en vach.

2.2. Ag2SO4 trong H2SO4: Can 5.5g Ag2SO4 cho vao trong 1 kg
H2SO4 (d=1.84 g/mL). e nguoi 1 2 ngay cho tan hoan toan
Ag2SO4.

2.3. Ch th ferroin: Hoa tan 1.485g 1,10 phenantroline va

0.695g FeSO4.7H2O trong mot t nc cat, them nc cat en
vach 100 mL.

2.4. Ferrous ammonium sunphat (FAS) 0.01 N: Hoa tan

3.92g Fe(NH4)2(SO4)2. 6H2O trong 500mL nc cat; them 20ml H2SO4
(d=1.84 g/mL) e nguoi. Chuyen vao bnh nh mc 1000mL,
them nc en vach. Dung dch nay khong ben nen phai
kiem tra lai nong o moi ngay bang chat chuan K 2Cr2O7.

2.5. Kiem tra lai nong o FAS: Lay chnh xac Vml dd
chuan K2Cr2O7 0.1N them 2ml dd H2SO4 va vai giot ch th
ferroin. Chuan o bang dung dch FAS en khi xuat hien mau
nau o.
Tnh toan nong o FAS: C FAS

C.V K 2Cr 2O 7
V FAS

vi VFAS: the tch dd

FAS chuan o c

D. BAO QUAN MAU

Neu mau sau khi lay ve c phan tch ngay trong vong 6
gi th ch can lam lanh 20C 50C.
Neu phan tch trong vong 5 ngay th axit hoa mau en pH<
2 bang H2SO4 (d=1.84g/mL) va bao quan lanh 20C 50C.

E. TIEN HANH PHAN TCH

1. Cho vao ong nghiem:17x100mm

 Chnh xac mot the tch mau nguyen hoac a c pha

loang (3ml)
2 ml dung dch K2Cr2O7 0.1 N va
3.5 ml dung dch Ag2SO4 trong H2SO4.
2. ay nap, lac eu, cho len bep un nhiet o 150 0C trong
2 gi.
3. Sau thi gian phan ng, lay ong ra khoi bep, e nguoi
en nhiet o phong, chuyen toan bo dung dch vao erlen,
trang ky ong nghiem bang nc cat, gop nc trang vao
erlen.
4. Them 2 3 giot ch th ferroin. Dung dch co mau xanh lam.
5. Tien hanh chuan o bang dung dch FAS 0.01 N. Ghi lai the
tch FAS a dung.
6. Lam mau trang vi 2-3 ml nc cat thay cho mau.

F. TNH KET QUA

Nhu cau oxy hoa hoc tnh bang mg/ L theo cong thc:

COD (mg / L)

V0 V1 * N * 8000
Vmau

Trong o:

V0 : The tch dung dch FAS dung chuan o mau

trang (mL)

V1 : The tch dung dch FAS dung chuan o mau (mL)

N : Nong o FAS a c kiem tra.

Vmau : The tch mau a s dung (mL).