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    cytogenetics

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    © All Rights Reserved
    Download as PDF or read online from Scribd
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    34 views22 pages

    09 85

    Uploaded by

    czumoffen
    cytogenetics

    Copyright:

    © All Rights Reserved
    Download as PDF or read online from Scribd
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    of 22
    RESEARCH ARTICLES Improved Detection of Chromosomal Abnormalities in Chronic Lymphocytic Leukemia by Conventional Cytogenetics Using CpG Oligonucleotide and Interleukin-2 Stimulation: A Belgian Multicentric Study Pete Vt at Sees Conese gk plana ‘ua aol Yor aa Ane Hagen Pr tna {te Bejn Cyopnte Guyer Horm Oneangy BEGG) ‘mn Peace ne om etre rina cence Sime Tiga Ul Sr aa Cri de ov ak Bas ‘aed shmates 17 cnmone ted era che pete (0) oredr ra ‘oj loer aoe tee Saga 3) ar cara nee stn 045) Pl ah of iperHoed iensctow mew top nh cto ot i cmon esl nog fa Orson ete (cr 2 ominaon of C6 egaucccce (46) tl rho OM ems weep i nc Go rate el} ce Be rs ea ITs CHGHLS care cnr TP ate 0 ce Ce heron re aed enone ein Cp? or Ph n 3915) nd (5) ea tarry ogee Ini hyegps war ted nh HEAL? ns DOL wi OA P< 000) pein f= 0) “hed te en areas wot by comes gets exes St) (9 7, in ei 6 ati ha f= oman rn sn oC [eprom ante oad RS Howe arctan for Feed al eran ‘crag compan orc a sas 69 Why Innopuerion reveling sora aryuype in 40-50% of ee Numesus sles base shown that dhe pres atsn eta 190). The ate of dete es ca Nantes ind ype af trance see, be laren to BS hy interne FSH her Sow’ repesch an iadpsnent pote! & 200). However, the ater tense Prognosis to Brel choi hpi leukemia tesony pail infertion conned to the he (CEL) Goose Ye Dc xan, msl ens examined. Po this enon, thee Daye oy 20K, Van Ben Nee ety 207) Thaler etopsnci lye i rowley psc ia tha dene exity, Homerer CLL end 0) Dhue aeaate “C1PA}, “ipopalyexchare {EBL pkcwend igen WAI sn pene Rend yp ie Sar vin hove boon eed te ees co Souitaigezat improve the ick of aberanemeapince, winch eyo Sete onvons “Ser _mapnertnes _CoGA2 AGG Foieior ao GL Sit 2 foe NO 'S femench ” Sege Mt Tea Sample cel wis dtmined ing a Sino cqvilene oie FISH CT 1). aut coe facet “Comer, Mi Unt vn yan fp compel te ul Nam, el. Deas en ee sok Chelacd ys now cette) wah Nt Relea, Dello ee ong TPA (Saki cea, 200) “Theres we pet ell om psigho tno (0 = 37) a famed amen yas the impett of fone mar 13), wee naw PT tiesedfeen ctor pceies onthe detection [949 mum wit) HEPES adage Sf dont soma eave soning THe (Laon Region stele as Setotiie pesca acniy rc canton peice Wek Tagen oneal wit ‘ims + gabon Tor conventional {9h mi pens ea tarpompca (koe, “gence none nC Tnvieagen Capac, Neste, Belg) No (Got sfamon paces out eplid Dale ATERIALS AND METHODS Seer we rap wich the olin oir {TPA GirAldich. Steinheim Cevtasy) or Pet an Spee G6 (Tip htt, tin; Gemma) sad 12 hs wiraretorpecve(rhce center and peck, onto, UR) The towing Bal pspetve (rw exten} mulcete dy concent vt (0) TPA. Heo Fevonel in ve Bean gence comer car. (vo center US pn (ne eee), Ol a Fondy ig CpOM2 foe snutin af CLL wo center) and (2) Cpr ty and U2 100 CllenerBerwees Oater 207 snd Nore” Ul al semen). Asding tal pati pol ter Bak, 217 comer antec ones, hen difeem coceteinn TPA wore we ‘Sled fr tine amis of CLL cies Seah the mandy ws rewopetve fr tne. centr ‘ens (= 172) o dng falwop (r= 45) Smpes wee incubated for 72 at 3P°C an ‘eve cane ncn sata wre () digo: $460. nly huni seep, Cae "of CLL and (2) asssment by" punllel mid (Gen, Invivogen Coroeion, Neelbske, seams it ati Sr man yn pone inn ocr wie sremiey NL oN pD se 9 9) wns NN a os ew Nf inet ee Sg nD Fa ERT NE EN THOT PELE lS RRND BAT NN, BAL pu A = @ x 2 = ow osha not 2 8 ob bh Bm sei er) eo Rw Mw ows aoa 3 8 4 te elt 7 oe 4 88 ‘in omg ‘aaa e 2 2 fae f bp 4G & pe an ah ne (ed 9 no eo ect neta Yu canes rans eo OR Heevinesafedn coon ranwe(aame) WS eX eB ‘Onst a “lb mmatrae nC 8 ot 8 Song mae tie 8 8 4 mi a ooo rere oo? ooo Ek FE dn ron erase ee ‘Nowa cea Td cytes z 2 s ‘ser eeanasine a4 ‘teeta ecAn pe) noo ‘ econ i * eA dean an ego?) a * Coes Cok ot ont) & % FEST perme re heater tome tren) rr peak Sabena feng hot oa 4 ‘al Ts + oe 4 8 8 Saha Gea or Seat a 3 fg 8 Teyahea moF fe 8 aut rxi cr ee nd Sectea a ob gy Foe ‘ion To y 8 Ube Sin eects) 8b Gf ae bof § 6 site eyes re iat tren rs Bison tay re “Econ p26 BS of fo todo ran ese: es) . ee ‘owica isto TH 5 5 oe rate wee 8 oe cn aun AG 85 bt cr as C72 Boi ft “avait 18 mal mee aa dn erly pe geenne Teale a, Riotacaarwscwncsten ere heneisce we ounces eum a le fr 1040 in tere ve Some) a in sgn poprtion of iespase Tae enti cobbisie aka SigmeAldich, sul by ierpase FISH. Fare was dtned Torn Hg) mas el ingead af Cleemid’ sls than 10 normal mete inthe ire Chomosome papain, ining pd meuphase under cosetaion, where sccess yas defined Suse bth clr were peste war sr 1D ome a eta ee peeoce igen mentrd pools Gh dana sinermaity he the ane f he Toe cones paral cnt clues wer set fests were In pt especie, ata of oy pith ppc Hed om fe hes volun 15-20 metaphases was amb in wo ene ‘omg cove uso 8 the (Center 2 and 5) ot ening te asia of vier eta To'metaphotes pr cle Chota abertons en Pliemton capac wk exited eg mana conn of etaphnos yrs he enter Aree lsd notice pops 18 mito Sid, 1-20 mioseasde apd >20 its Karyorypes vere desi asain 20 1SCN 0s (Sater an Terme, 200) Niele fe aarti were deed a those stint fri egy dei Hate fate Sl end treaton Ado! Sheri wks ined in (he pomp a mincing aber to Soaed wa trmicatinClnay woe MA of bai {nined ay the pesene esr nas "The cases were casi in dice groupe rpm ofan ene chonmmame i tft evo goad feed, ae gor, asarding tothe Miciphaes arf oan cure chenese i at Sly of banding th ept the number of Iu te wesennes When pa sera: chenoanol ane per Ingo katy, ‘ln ws nce i ely one meu, H's S30 tad, 200-0 bay and 00 ba Smid ss onl wher conned in at lat expect Tbe name f bas ws estimated Soe menplase nthe pul elie (ee two With Mlagams of nding pate (Sater and Imcposer nce f fw af thle chrome. "Tommie, 25 Incrpee HM Annee Forno sd pat fess, FISH was Cotes Only in Cane ex an cen yi other ecotes weed teas Shed The flowing ‘commercaytatble fics Cll fom Vy, Downe rove, Ti} vere wed to hepato lod commeniy faved in CLL: 2h (LST IYBL. 1g (St [NDA ecnmere 12 (CH 12), 15g S13 RI conned with LSU DLSSHY 142 (81 Tat at clobteak spr, and 1718 (LS 138 ened ith CEPOL. Atte tie of “amples from patient age fess than 70 yeas. In (Gomer 1the pane cade i probes. ee ‘he pel ded for pao. Le 2, som teeter 12.114 and 3p On flop se eran inpatient aged me tha 70 Ye, Incase FIST wat peared ecning Tal proce pai. The alse were Bete ened cer on unaired spl or on oe SME TPA w GhGML-2 Guroff value wore ‘Sublated n cath cee foreach ee. Fat tied and lsc sample ad fr psp ‘cal Mandan bone me, the sie eae Unaiscd ileences between TPA. and (Gye? were ened wi the MeNem test plied for esing prion capaci and inva nding (Nera, 97: Apes, 202 ‘Diferencs, ajowed for comer and tae, vet einstein ube pti isin enced near med modes wh» ado, Fee or pont and feo ak poroptin,wsing sxinun Hkelbod (Aust, tn adage Caner ques i 7 Points wit aed fo inepte ower the ao Teepe west sere indeted (Ages, az; te et, 2008) Estimates even ‘ated ith fine ltl which "Comer for cemter, "base mow” forse and "CpGtts2" foe meio. Deis we show a Sopot aston Tales 19. Rls were ome scat athe 0S level ‘Some india abceaions were fund he tte Gene in toby *) We here foe only sept mated re ing the Neer tt ar these (Aes 02 ‘Conon! Cyogoete Alas Amd of 20 metaphases (ange 8-61) coud be eqegenetiaiyanaeed ia 214 of 217 ees (rly. Tae (JeSned above) occred in 42 fsa afer TPA (secs ate 0 B18) adn 35 fees afer CpOMLZ eultve (neces fate af ip, No simian aitteence in freloe fest sate for ether simulation technique as nerve (P= 0082), Comers 2 sind 8 wee si ‘amy more likely o bute # ale ow Se, ths eum be explained by bea! ices conceting the number of alyeed ‘fap per clare. Of note, Ceutr# ans Iprng the bighest number af metsptane pet Str had the highew detection nite of sbaoe ‘nls, Detaled veut pr enter a shown iTable ‘Con sbnomaliies were cbscred ia 16 ste (8) In Mees (3) he sero elone hs tered fn bth cates, OF thee he po vas of sberen mcapases were sn it vlc 17 and higher CpG? ad JTPA cates in aod if can reget (One sbeant sone war doce in af thee ‘Messe Al bit ae cle wee en ath (Glow conn, OF 22 crs wah more then ne wbslng the sbelnes ere relat at ‘Stace br cach, One sion urelaed Selone as found aly afer stilton with Gp? oe TPA fora oe ees, rrp ey. In one ee, ll sbelnes oberved in CaCl? sod TPA cules were vorelaed. In fe detested onl in Cx? on TPA clearer rspasively Ts the peeenige of anormal Kenyeryper wth: CHCHILZ and TPA vas SIs nd Se egecvly (P< ODD on, (Crit? tres were siiieay oe ely to yal higher umber of abortions {ode Dee on amber aeration pe fare and on kareipe cme ae shown ih The lowing aboomatie were detected ser Cpe? and TPA. smulaton, espe: cyl) Gr 1 a I PR) 1s dy Cnet nee As er e233 Gi@IADY BGMRF inser Bt Maia 1 ORS tate Beate conse Bs Reintaeay TT ai sarang wo scien ' 2k Binnie an Shea : Sian me Sieane i Solis st sie 3 Sechampaet) et Sikes i ‘io mt May at ‘Cae mf oingleane aoa et et Me} etait 2 2m le 3 ‘tisleto i iil Seta a} aa: 2 Sub e 3 sisi — oho 3 Stele ; cenwionr 2 simon, : sia aimee i angen ; Sinerar ‘ isielai? ' flan eit! ar ‘ Came 4 ‘Sacra setts 4 fetpiate) : rine a i seit) 3 Shearer : toe ‘ ‘Sctopemeras i Hei i soya ' eta t seine ‘ seminar t set i eon, } Sects 3 vet Soarbiam cian i ei gar er decrniseintranain st Hi Smaart siete im Hi Stawernetiats "st hina ‘ot fa Sith meta et Sete natn so Te Statist sete } Be Slee ey teeta a et te trian, “ot ats oot matey ‘eto et om intone aoa weir ca eee md 3 teiheatar at al fonction et Bh Seeaiematy tt at Gian mt rae ia aiatieansy eT ee fet J settee ie Conary St et en ann _ ela D1 x pats _demenniann mo tdi Ge — 200028, P08 det Ge = 21 ve Pen ie sbemionn Ov 2 mh ‘cluding delim tslosons ada P= (dep) vn Boe, Pe [and dee SB ye P= 018" “Teast afer CAGE cum 80 ees (P42, respectively (les and). The aces in tein rte er Cpe? san as si for bath laced (4 32 17) and unbalanced (n= 2 1) tandoctios. "Voeston® sce ‘sh Bell Iympoolfeve dsrder were here i ight eae HL4IBX 32320 (= 3 aioyahdghs) Gm Zand one each of Sets nate “nd id (Glgi2 We aid oe mgt to crew al Janos se to be exfeoed heetse diene nding tiga (Ge Q) wee sed i ferent come Neverban, the bekpoite Teat-14 apa to be highly scuseny ing “hicred in" 13 eatslcatins. Sone of these, Sch #2919 cad be eee ia “ifr pte. Means aban were ‘lected inh cme 3 cact (P= OLD) aor (Gaited wd 'TPA simu, espe. periph! bled fom Be belly tla teem 30 maps were examined pe ae fev se: In ect smal with CpG, Di sieewtnes wee Se ct fe te ‘oat ING28A) and pin of & noon Smiter ieee emted Sar ssne were ined wih TPA, ta rondonlearlcion {We1d{q S52) snd nonlnl wmy 3a one sermons wore ial in fur eons 19) Micellneos wolves Were Ser C52 sinlion P= M3). Among thc onal andes wore Steed in Her proprio Cp than a TPA cue tues (P= 05), en G25) vem ener (28) repetely Tae, Plt capacty Prieto capacity wa eed in ttf indo ws sy higher Cit tha ib eon 2, 15-20 mses in 18 ves 1 Ghd

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