Chemistry and Biochemistry 153L Winter 2015

Biochemical Methods I Introduction to protein science

Instructor: Anne Hong-Hermesdorf

Lecture:
T, R 11 11:50 am: YH CS50
Office hours: T 12 -1 pm:
YH 1049
F

12:30 1:30 pm: YH 1049

Teaching assistants and Laboratory Sessions:

Texts:

Session

Day

Start

End

Room

TA

1A
1B
1C
1D
1E
1F
1G
1H
1I
1J
1K
1L
1M
1N

M
M
T
T
W
W
W
W
R
R
R
R
F
F

1:00P
1:00P
1:00P
1:00P
9:00A
9:00A
1:30P
1:30P
1:00P
1:00P
5:30P
5:30P
1:30P
1:30P

4:50P
4:50P
4:50P
4:50P
12:50P
12:50P
5:20P
5:20P
4:50P
4:50P
9:20P
9:20P
5:20P
5:20P

YH 1049
YH 1067
YH 1049
YH 1067
YH 1049
YH 1067
YH 1049
YH 1067
YH 1049
YH 1067
YH 1049
YH 1067
YH 1049
YH 1067

Kelly Quinn
Sue Tsui
Hanjun Kim
Joanne Leung
Jarrett Miller
Keith Cheung
Alex Bradley
Huimin Zhang
Jessica Davis
Adlin Scott
Michael Chambers
Michael Leonard
Joseph Cao
Uland Lau

Introduction to Protein Science Laboratory Manual, J.W. Gober, 3 rd edtion

(2014) (available at Course Reader Material on 1080 Broxton Ave) and any text
book in basic biochemistry (not older than 5 years)

Grading: Midterm examination

Final examination (cumulative)
Lab preparation protocol (8 total, 5 points each)
Lab reports (7 total; 20 points each)

100 points
200 points
40 points
140 points

3 points may be earned as extra credit for evaluating the class (anonymous)
No make-up exams will be given. Check your schedule, if you have a conflict, do
NOT register for this class.

Monday

Tuesday

Wednesday

Thursday

Friday

5-J an

6-J an

7-J an

8-J an

9-J an

LECTURE 1

LECTURE2

NO LAB

NO LAB

NO LAB

NO LAB

NO LAB

12-J an

13-J an

14-J an

15-J an

16-J an

LECTURE 3
LAB 1/ Start
LAB 2

19-J an

LAB 1/ Start
LAB 2

20-J an

LECTURE 4
LAB 1/ Start
LAB 2

21-J an

LECTURE 5

LAB 1/ Start
LAB 2

22-J an

LAB 1/ Start
LAB 2

23-J an

LECTURE 6

Holiday

LAB1/ LAB 2

LAB1/ LAB 2

LAB1/ LAB 2

LAB1/ LAB 2

26-J an

27-J an

28-J an

29-J an

30-J an

LECTURE 7

LECTURE 8

LAB 3

LAB 3

LAB 3

2-Feb

3-Feb

4-Feb

LAB 3

LAB 3

5-Feb

6-Feb

REVI EW FOR
MI DTERM

LECTURE 9

COMPUTER LABCOMPUTER LAB COMPUTER LAB COMPUTER LABCOMPUTER LAB

9-Feb

10-Feb
MI DTERM
EXAM

11-Feb

12-Feb

13-Feb

LAB 4

LAB 4

LAB 4

LAB 4

LAB 4

16-Feb

17-Feb

18-Feb

19-Feb

20-Feb

LECTURE 10

LECTURE 11

LECTURE 12

Holiday

LAB 5

LAB 5

LAB 5

LAB 5

23-Feb

24-Feb

25-Feb

26-Feb

27-Feb

LECTURE 13

LECTURE 14

LAB 6

LAB 6

LAB 6

LAB 6

LAB 6

2-Mar

3-Mar

4-Mar

5-Mar

6-Mar

LECTURE 15

LECTURE 16

LAB 7

LAB 7

LAB 7

LAB 7

LAB 7

9-Mar

10-Mar

11-Mar

12-Mar

13-Mar

LECTURE 17

FI NAL REVI EW

LAB 8

LAB 8

LAB 8

LAB 8

LAB 8

16-Mar

17-Mar

18-Mar

19-Mar

20-Mar

FI NAL EXAM

be prepared before you come to lab:

Attend lecture
Read respective part of manual

Watch video(s)
Write in a notebook exactly how you need to do the
experiment, turn in a copy to your TA (does not have to be
carbon copy)
LAB PREP GRADING:
5 points are granted for your pre-lab reports; part of this is to
fill in the tables with concentrations (such as the BSA dilution
series on page 12)

laboratory reports
TEAM WORK, but INDIVIDUAL report; AFTER you gather
the data as a group, the reports need to be written entirely
by you.
7 reports (20 pts each); report your results and answer the
questions for each report in the end of each lab protocol in
the manual (due one week after the experiment is finished)