the insects of East Texas. In my particular eco region which is the Pining Woods eco region. And my research right now focuses on an area of forensic entomology. So forensic entomology is the use of insects to help us to estimate a period of time. And forensic entomology, deals with a lot of different scenarios but I think the one that's most famous is the use of insects in, areas or, or situations of, questionable deaths. So what we do is we use information provided about, provided by the insects. To work backwards through time to estimate, how long the insects have colonized the cadaver, and that period usually correlates to the time since death, or it can correlate to that period very closely. So some of the clues that we use, for example would be age of the maggot. A maggot is the larval stage of flies. And, there are several species of flies and, several families that are obligate feeders of decomposing fresh. And, the adults can come and go from the cadaver, but the females will lay their eggs on the body, and the maggots must develop. They must complete their life cycle on the cadaver. And the females will arrive at the cadaver, almost immediately upon death. And so, she'll lay her eggs almost as soon as she arrives. And so, that means that the, the maggot on the cadaver, was, hatched there, almost as soon as she arrives and so that, that age of the maggot correlates then to the sign, to the time since death. We can also use information about insect succession, or how insects will colonize the body in waves as decomposition progresses, through time and the body shifts from what stages, stages of decomposition, to dry stages of decomposition. Okay, so we see different insects specializing on the cadaver at different stages of decomposition. So for example, wall flies will colonize the cadaver immediately upon death, and the maggots will be active feeding,
while the cadaver is in it's
wet stages of decomposition. And there will be beetles that come and go, they're kind of feeding on the maggots, but they're not com, they're not really obligate feeders there. And then as the bio, as the body, dehydrates, you see a shift in insects that are feeding on the cadaver. And so I think, and a lot of my other colleagues think as well, that the microbes are behaving in a similar fashion. They're experiencing same sort of ecological succession being driven by this, primi, this very ephemeral food source and it's shifting from wet decomposition to dry decomposition. So, as the body progresses through decomposition, the insects change and then the microbes change. And so we think that, we can, we can get information about time, based on suites of organisms, microbes and maggots that are present or absent at particular stages of decomposition. >> So I do a lot of my fieldwork outside at am specialized, facility that we have available at Sam Houston State, and it's called the Southeast Texas Applied Forensic Science Facility or STAFS, for short. And, the STAFS facility is a state-of-the-art decomposition facility, that is set up in, in a remote location, and designed to study, human decomposition in a very controlled manner. And I've been working at the STAFS facility since about 2009, when it was first set up, with the director, Dr. Jan Bideway. . And, through the generous donations of families, we have been able to catalog, insect diversity on cadavers through time. And we've started to catalog the microbial diversity on these cadavers through time. And so, myself and my graduate students have a very thorough dataset of insects that colonize cadavers. Not just in one season, but throughout all the seasons. And this data set again it's particular to east Texas. This is a very, it's a very eco, each, eco, er, geographic specific science.
And so we have, we have,
one of the most complete data sets for insects, that are found on cadavers in this area. And we started to colle, to catalogue the microbial diversity as well. We have completed, a very, very preliminary study on the internal, community of bacteria during decomposition. And we were able to sample some sites that have never been sampled before. And so, thi, it was pretty exciting. And we started now to catalogue the external, microbe build diversity of, of humans in, a natural but controlled environment. And, this research that we're conducting is funded through the National Institute of Justice. It's a, it's a three year grant. We're placing two bodies per season, per year for three years. And we're swabbing the cadavers, at, specified time intervals. And we're collecting the insects that are present on the cadavers each time that we sample. So we're going to correlate insects and microbes to the accumulated degree hour, and then to each other as well. We have a number of different experts on the project. And I should start by mentioning the co-PI on my active grant, Aaron Linn. He is a microbe biologist who works at Sam Houston State, University, with me as well. And is, a co-PI on the NIJ grant. And we work with, on that grant really closely with, Dr. Joe Petrosino from Baylor College of Medicine. And one of his, former graduate students, Embriette Hyde. We also work with, the Knight Lab at the University of Colorado. We work with, Jess and, and Rob Knight. Our research program, has been succesful because we have been able to recruit, a number of wonderful undergraduates to this research project. And these undergraduates, frequently will transition into graduate school, and, and get their Masters Degree from the department of biology here. And, they are, they're, it's a fantastic training opportunity to work with the staff's facility. And to be involved in this interdisciplinary research object.
It's, it's a wonderful, it's wonderful for
these undergraduates, and then graduate students, to see all the different of this research project that we're kind of pulling in together. I failed to mention the chemistry, which is another big part of the project that we're doing is trying to catalog, particular, chemical byproducts during decomposition as well. And so they see, they see all these different areas, and they get to go out to the field constantly. They get this unique experience that other students just don't get. There are four, forensic anthropology research facilities in the United States. The University of Tennessee, had the first one. Texas has two, we have one, at, at was in West Texas, which is a very dry environment as opposed to the one that I work at, which is very, wet environment. Huntsville, Texas is about an hour north of Houston. And we have, temperatures and cli, climate that's described as subtropical. So we dont' really get, you know, we don't really get, too much snow, sometimes some ice, but it's mostly very warm, very humid. And, and so we can, we're at a very unique environment to study the cadavers under, but it's still very relevent to this big metropolitan area of Houston. So STAFS is a willed body donor facility which means that a person can donate their body to science. Or their family can donate their body to science. And we, we're very, grateful for these donations. Without these donations we wouldn't be able to do our research. And it's our research, I think, that kind of serves as a backbone and kind of puts together scenarios in anticipation of, you know, criminal events that, that might be unique. Or might require very, specialized circumstances to help kind of help interpret those, those sis, situations. Again at STAFS we're very grateful for these donations. And one of the things tha,t the director of the facility does
annually is a a dedication ceremony for
all of the donors and all of their families, to show our appreciation, for their generous gifts. that, that are research program relies on.