VKT - D12A

2/11/2015

PROBIOTIC

Cau truc giai phau ong tieu hoa

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2/11/2015

Ruot khoe manh

Ruot b ton thng

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Vi sinh vat probiotic

Vi khuan
lactic

-Chi Lactobacillus, Leuconostoc,

Pediococcus, Lactococcus
-Streptococcus: S.thermophilus
-Enterococcus

Probiotic

Bifidobacteria

Nam men

Hnh anh mot so vi sinh vat probiotic

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Nha san
xuat

San pham

Classic
Flavor
LGG

Danone

Lactinex

Hynson,
Westcott &
Dunning

Biola

Tine

LC1

Nestle

Con Agra

Lactobacillus casei

Vi sinh vat

Nhiet o toi u
(oC)

B.bifidum
B.lactis
B.longum
Lactobacillus
acidophillus
L.bulgaricus
L.casei

37-41
37-41
37-41
35-38

L.fermentum

45

L.rhamnosus

37

L.salivarius

30-40

Enterococcus faecium
Streptococcus

22-33
37-45

40-43
30-35

San pham len men

Acid lactic
Acid lactic

Vai tro

L.
acidophilus
L.GG

Tang cng
sc khoe

L.
acidophilus
L.
Bulgaricus
L.acido,
LGG
S.thermo,
L.bulga,
L.acido

Dang s
dung

Tang cng
sc khoe
Tang cng
sc khoe

Can bang he
VK
Tang cng
ap ng
mien dch

Sa chua
Vien nang
Bot

Sa chua
Vien nang

Tnh an toan cua probiotic

A. lactic, a .acetic, a. formic

A. lactic, a. acetic, a. formic
A. lactic, a. acetic, a. formic
A. lactic, a. acetic, a. formic
Acid lactic
Acid lactic
Acid lactic
A. lactic, a. acetic, a. formic,
etanol
A lactic, a acetic, a formic,
etanol
A lactic, a acetic, a formic,
etanol

Probiotics

Probiotic
Th nghiem

In-vitro

Thu

Ngi

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Tnh chat probiotic

Tnh an toan & hieu qua cua sp
Chuyen hoa
Nghien cu

oc tnh cap

tnh an toan
cua
probiotic
mi

In vitro, anh gia s tang

trng
Xac nh lieu toi a, toi thieu
Theo doi tac dung phu
Giam sat dch te hoc

NGHIEN CU

KET QUA

Nghien cu in-vitro
Kha nang xam lan

Gan ket te bao bieu mo ruot nhng khong

xam lan, ngan chan s xa m lan va canh
tranh vi VK gay benh
Khong

Giam lp mang
nhay
Tao chat khang
khuan
Th nghiem tren
thu
Hnh thanh khoi u
Ton thng gan do
ru
Th nghie m lam
sang

Chon loc vi sinh vat probiotic

Nguon goc: mieng, ong
tieu hoa, phan

Ngan chan VK gay benh phat trien

Vi sinh vat
Lam cham s phat trien cac khoi u
Ngan chan hay lam giam cac ton thng
Ngan chan cac benh hay cac roi loan chc
nang, cac tac dung phu hay cac anh hng
co hai

An toan

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Phan lap vi sinh vat probiotic

VSV

MT khong
chon loc

MT chon
loc

Chat bo
sung

k nuoi
cay

Lactobacilli

MRS

SL

Cystein, yttt,
carbohydrat

10% CO2

Bifidobacteria

MRS

TPY

Khang sinh

10% CO2,
k kh

Streptococci

Strep base

TYC

5% CO2

Enterococci

BHI

Kanamycin
aesculin

> 3% CO2

So te bao
Ton tai, phat trien trong ong
tieu hoa

ac tnh
sinh hoc

Chu c pH thap, dch tuy,

dch mat

Ban au

Sau 3h

L.casei

2.0x108

1.0x108

L.rhamnosus

4.5x108

6.9x106

L.acidophilus

1.5x107

5.4x104

L.bulgaricus

9.6x106

S.thermophilus

2.6x106

6.1x103

So te bao VK song sot sau 3h trong moi trng

dch tieu hoa

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Goc vi sinh vat thuan chung

Hoat hoa
Cay vao moi trng thch hp

Nhan giong
Kha nang thch
ng mt dch mat
cua VSV

Nuoi cay

Ly tam thu sinh khoi vi sinh vat

Ra sinh khoi bang nc muoi
vo trung.

Bao quan vi sinh vat di dang nguyen lieu

PROBIOTIC

Gan te bao bieu mo ruot

VSV

SL VSV
banau
(CFU/ml)

Canh tranh VK gay benh

Co tnh khang khuan
ac tnh
c hoc

Kch thch ap ng mien dch

% VSV song sot

Ngay
14
28
4oC 25oC

49

4oC 25oC

4oC 25oC

L.casei

1.6x108

100

93.8 87.5

93.8 62.5

87.5 57.5

L. rhamnosus

4.8x108

100

64.5 25

10.4 7.1

L. acidophilus

1.7x108

100

25.9 2.3

1.9

0.03

0.008 0

L.bulgaricus

5.2x107

100

67.3 21

1.2

0.002

0.06

S.thermiphilus

3.2x106

100

10.3 0.013

0.25 0

1.9

Kch thch VK co li phat trien

c che VK co hai

Bao ve hang rao ruot

0.072 0

Kha nang song sot trong mt sa cua VK kbq

4oC va 25oC

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Vai tro probiotic

Con ngi

Chan nuoi

Gia suc

Thuy hai san

PROBIOTIC

Dinh
dng

Can
bang he
VSV

Bo
sung

Acid
amin

Hydrat
carbon

Acid
beo

Tac ong

Probiotic

San xuat vitamin tan trong nc:

thiamin, acid nicotinic, acid folic,
pyridoxin, vitB12

B. bifidum
B.breve, B.longum
B.infantis

San xuat biotin

B. bifidum B.breve,
B.longum
B.infantis

Gia tang hoat tnh sinh hoc cua sat

L.acidophilus

Tach acid taurocholic va acid

taurodeoxycholic (muoi mat)

L.fermentum
L.reuteri

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Vai tro dinh dng

Vai tro can bang he vi sinh vat

Protein

Triglyceride
LIPASE

PROTEINASE

Ngan chan vi khuan gay benh: Salmonella,

Listeria, P.aeruginosa, E.coli
ieu tr tieu chay
Sa (n=24)

Yaourt (n=21)

1000
1112
1850

853
445
1430

1.2

2.1

Acid beo + Glycerol

Polypeptid

Lng phan long(g)

Lng ORS s dung (ml)
Tong nang lng cung
cap(kcal)
Tang can sau 5 ngay(%)*

POLYPEPTIDASE
Acid amin

*: so vi trong lng ban au

Nghien cu tre em t 6-14 tuoi

Thong so

Lactobacillus
(n=21)

Placebo
(n=19)

Tuoi (thang)
Can nang (kg)
Thi gian tieu chay (ngay)
So lan tieu chay
Ngay 1
Ngay 2
So lan oi
Ngay 1
Ngay 2
Tang can
Sau 24h
Sau 48h
So BN tiep tuc tieu chay

11.5
6.9
4.5

14.4
6.8
3.8

8.5
5.8

10.5
7.0

5.6
2.0

5.8
4.0

-0.6
+1.0
5(31%)

-1.4
-0.9
12(75%)

Lactose
LACTASE

Glucose
Acid pyruvic

Acid lactic

Galactose

Nc

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ieu tr va ngan nga tao bon

Anh hng nong o cholesterol

oi tng

Probiotic

Ngi khoe
manh
Ngi khoe
manh

L.bulgaricus
S.thermophilus
Enterococcus
faecium
S.thermophilus
B.subtilis
L.acidophilus
L.casei
S.thermophilus
S.cerevisae

Chuot cao
choles

ng s
dung
Uong

Anh hng

Uong

Giam choles
mau/1tuan
Giam LDL

Uong

Giam choles

E.Coli xam nhap te

bao ruot

S.Typhimurium
xam nhap te bao
ruot

C che tac ong

1. Canh tranh oc quyen

Nghien cu kha nang bam dnh cua vi khuan

probiotic tren te bao ruot Caco-2 (ATCC HTB37)
hay Intestin 407(ATCC CCL6)

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Te bao ruot

Probiotic

Nuoi trong RPMI 1640

Nuoi cay

Ra bang BPS

Ong th

Ong chng

Te bao ruot u vi
huyet thanh cha
khang the anti-SplA

Te bao ruot u vi dung

dch PBS

Ra te bao vi khuan
probiotic bang dung
dch PBS, khuech tan
trong moi trng RPMI

Ra te bao vi khuan
probiotic bang dung
dch PBS, khuech tan
trong moi trng RPMI

Khuech tan trong

RPMI

Ra bang BPS
Co nh MeOH

Xac nh so te bao bam

dnh

Knh hien vi

2. San xuat chat khang khuan

Bateriocin va cac chat tng t

Khang sinh
Acid lactic
H2O2

Cau truc thanh te bao vi khuan

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Saccharomyces boulardii trong ong tieu hoa

Saccharomyces boulardii canh tranh vi

Clostridium difficile

V tr tac ong cua Saccharomyces boulardii

Saccharomyces boulardii tao thanh hang rao

bao ve niem mac ruot

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Cac chat khang khuan phan lap t Lactobacilli

Chat
Acidolin
Acidophilin
Bulgarin
Lactacin B
Lactacin F
Lactibrevin
Lactobacillin
Lactolin
Lactolin 27
Plantaricin A
Plantaricin B
Plantaricin SIK-83
Reuterin

Vi Khuan
L.acidophilus
L.acidophilus
L.bulgaricus
L.acidophilus
L.acidophilus
L.brevis
L.brevis
L.plantarum
L.helveticus
L.plantarum
L.plantarum
L.plantarum
L. reuteri

Lactobacillus GG kich thch thanh lap khang the

3.Kch thch ap ng mien dch

nh lng khang the
Lay mau chuot tai 3 thi iem: trc khi
uong,trong khi uong va sau khi uong probiotic
nh lng te bao lymphocyte T, khang the CD,
IgA, IgM, IgG bang phng phap mien dch huynh
quang hay o dong chay te bao
Dung phng phap thong ke anh gia s khac
biet ve lng khang the tai cac thi iem

He thong bao ve cua ruot