BIOASSAY CHECKLIST

1.
2.
3.
4.
5.
6.
7.
8.

Animal
Animal Cage
Animal Tray
Rod to Stun animal
Gloves no.7 ( x6 pcs )
Bucket, Mug and Heater coil
DW 5L flasks x4
DW 3L flasks x2

9. Instruments tray
a. Tooth forceps x2
b. Plain forceps x2
c. BP handle with needle x2
d. Small straight pointed scissor
x2
e. Artery forceps small x2
10.
a.
b.
c.
d.
e.
f.
g.
h.
i.
j.
k.
l.
m.
n.
o.
p.

Central Tray
Petri dishes x4 with markings
Scissors straight pointed x2
5ml Pipette for wash
100 ml cylinders for warm
tyrode
Needles straight x4, curved x4
Thread for needles
Tyrode flasks x2. Marked for
volume.labelled
500 cc beakers ..labelled Tyrode
250 cc beakers labeled
Tyrode
25 ml pipette for Dilutions
Glass Stirrers x2
Two 10 cc cylinders for
i. Stock
ii. Unknown
Syringe-needle for each (x2)
Sticking bundle
Atropine vials x2
10 cc syringe with 24G
needle labeled atropine

11.

Assembly (2 nos)
a. Lever x2
b. Clay for weights
c. Organ bath, Heater Coil
i. 3 coils, Inlet tubing and
two outlet tunings
d. Reservoirs
e. Stand for reservoirs
f. Glass rod to mount tissue
g. IV set for aerator x2
h. Aerator x2
i. Motor Stirrer x2

12.

Tyrode Salts tray

a. All Salts
i. NaCl, NaHCO3, Glucose, KCl

Indent animal 2 days prior

Clean cage

Prepare markings

Heating of warm tyrode preparation

Label flasks, mark volumes

Clean Bath and coils and instruments

Plug points required

a) For drum x2
b) For Aerator x2
c) For water bath heater x2
d) For stirrer x2
e) pH meter

ii.
iii.
iv.
v.
vi.
vii.
viii.
13.
a.
b.
c.
d.
e.

14.

CaCl2, MgCl2, NaH2PO4

Histamine
Butter Paper
Plain Paper
Scoop x2
Stirrer x2
Brush for dusting

Smoking of drum / pH meter

Chart and Glue / Fevicol
Kerosene
Swab for burning
Matchstick
100 cc beaker . Labeled DW
(pH)

Experiment Tray (2nos)

a. Scales
b. Stopwatch
c. 8 Cylinders labeled properly
(4 D, 4U )
d. 8 syringes needleslabeled
e. 100 cc beaker to hold syringes
f. 100 cc beaker. labeled tyrode
g. 50 cc beaker .. labeled DW
h. Glass Stirrer
i. Thermometer with holder
j. Pencil for writing

15.
a.
b.
c.
d.
e.
f.

Fixing Tray
Scalpel blade
Plastic gloves
Cotton
Resins
Glycerine
Spirit

16.Graphs papers / Log papers

RABBIT EYE EXPT

1. Apron
2. Gloves x 2 sets x 2nos = 4 (for
assistant)
3. Scissors curved x1
4. Straight scissors x1
5. Marker Pen x1
Miosis /Mydriasis expt
6. Flasks x2
a. For atropine 1%
b. For Normal saline
7. Droppers x2
8. 1ml syringes as droppers x3.
Labeled for NS
9. Needle of 18-22 for withdrawal in
syringes
10.Atropine vial
11.Homatropine / Pilocarpine
12.Phenylepherine
13.Torch
14.Pupillometers

Labelling required

Lignocaine Expt
15.Lignocaine vial with needle n syringe
1cc
16.Cotton wasps

RABBIT EAR EXPT

1. Apron
2. Gloves x 2 sets x 2nos = 4 (for
assistant)
3. Shaving blade and handle
4. Straight scissors x1
5. Spirit bottle
6. Xylol bottle
7. Picric acid
8. Cotton plugs x 8pc
9. Bulldog clamp
Ear vein collection
10.Heparin
11.1cc syringe with 26 no needle x 6pc
12.2cc syringe wit 26 no needle x 6pc

GUINEA PIG EXPT

1. Apron
2. Gloves x 2 sets x 2nos = 4 (for
assistant)
3. Trimmer for hair
4. Shaving blade and handle
5. Straight scissors x1
6. Marker
IP injection
7. 2cc syringe
8. 18 22 no needle
9. NS flask
Anesthesia
10.Lignocaine jelly
11.Lignocaine vial
12.2cc syringe with 24 no needle
13.Needle to test sensation