Professional Documents
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(52)
(75)
Inventor:
(Us)
(73)
Assigneez
(57)
VANDERBILT UNIVERSITY
Nashville TN (Us)
ABSTRACT
(21)
Appl_ No;
13/299,241
(22)
Filed;
N0 17, 2011
(60)
ciently small such that an electron beam that enters from the
?rst WindoW can propagate through the ?rst sub-chamber and
exit from the second WindoW. The at least one second sub
Pubhcatlon Classl?catlon
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equacies.
[0001] This application claims the bene?t of US. Provi
sional Application No. 61/414,603, entitled Transmission
Electron Microscopy for Imaging Live Cells, ?led on Nov.
17, 2010. The disclosure of the above application is incorpo
rated herein by reference in its entirety.
[0002] Some references, Which may include patents, patent
applications and various publications, are cited and discussed
in the description of this disclosure. The citation and/or dis
cussion of such references is provided merely to clarify the
description of the present disclosure and is not an admission
that any such reference is prior art to the disclosure
described herein. All references cited and discussed in this
[0003]
second WindoW. Both the ?rst WindoW and the second Win
doW are transparent to electrons of certain energies. The sec
[0010]
transparent WindoWs.
[0004]
one embodiment, the method includes the steps of: (a) placing
nal ?oW system includes means for exchanging ?uid With the
micro?uidic chamber. In one embodiment, the external ?oW
system includes means for providing nutrients to the cell in
the micro?uidic chamber. In another embodiment, the exter
nal ?oW system includes means for injecting chemicals into