Which brand of bottled water contains

the most colonies of bacteria.

Abstract
The point of this experiment is so that we can find out which brand of bottled water is the
cleanest bacteria wise. You will need Paddle testers, different brands of bottled water (we used
nine in this experiment,) a room temperature incubator, and a control, which would be any water
that you are certain will have bacteria. We will be using paddles slides to cultivate the bacteria.
Once we place them in an incubator, small oval or circular dots will appear in different colors
(such as white, red, tan, yellow, black, etc.) These are colonies of bacteria. In order to count
each bacteria you would need a microscope so for the purpose of this project we will only count
the colonies. Our results were: Control- 48, Aspen pure- 7, Fiji- 0, Evian- 0, Smartwater- 0,
Arrowhead- 1, Propel- 0, Dasani- 0, Niagara- 0, kroeger- 0. One problem that we ran into is the
paddle testers, the ones we used for this experiment were expired so the results might it be
accurate. We ordered new ones but they were placed back order. They wont get here until
November so when we get the we will redo this experiment.
Why this experiment?
The point of this experiment is so that we can find out which brand of bottled water is the
cleanest. Claire Bedford did an experiment in 5th grade where she had to determine which type
of water (out of river water, faucet water, bottled water, and filtered water) contains the most
bacteria colonies. also, The Telegraph states that there is more bacteria in bottled water than
tap water. We will be using paddles slides to cultivate the bacteria. Once we place them in an
incubator, small oval or circular dots will appear in different colors (such as white, red, tan,
yellow, black, etc.) These are colonies of bacteria. In order to count each bacteria you would
need a microscope so for the purpose of this project we will only count the colonies.
Hypothesis
If bottled water contains bacteria than we believe that Smart Water will contain the most
because they stated on their site that there were bad types of bacteria in their water.
Materials needed:
-Paddle testers
-Different types of bottled water
-Incubator

Procedures
First, you buy 9 different types of bottled water (for this experiment we used Aspen Pure Rocky
Mt. Water, Fiji Natural Artesian Water, Evian natural spring water, Glaceau Smart Water, Arrowhead spring
water, Propel water with electrolytes, Dasani purified water, Niagara purified drinking water, Kroger
purified drinking water. Then, you pour some of the water from each bottle into separate

containers. Next, you put the paddle testers into the water and leave them for three minutes
each. Last, you pull them out and put them back into their container and place them into an
incubator that keeps room temperature. Once this process is done and you leave the paddle
testers in the incubator three days, you pull them out and count all the colonies of bacteria on
the paddle. Be sure to list your results!
Results:
Control- 48
Aspen pure- 7
Fiji- 0
Evian- 0, Smartwater- 0
Arrowhead- 1
Propel- 0
Dasani- 0
Niagara- 0
kroeger- 0.

Research:
Aspen Pure natural water.
At the bottling center, Aspen Pure natural water travels through a five-step filtration and
purification system. Upon completion of the filtration process, the water is ozonated and then
the bottling process begins. The bottles are filled with water, labeled, sealed, inspected, packed
and prepared for shipping. The end result delivers customers clean, high-quality bottled water.
Fiji natural water.
Industrialized country. It is bottled at the source in the remote Yaqara Valley on the island of Viti
Levu.ne hundred percent of FIJI Water is from a single source in the pristine, tropical Fiji
Islands, an archipelago of over 300 islands nestled in the South Pacific, more than 1600 miles
from the nearest industie.

Evian natural spring water.

The origins of evian Natural Mineral Water date back thousands of years to the very birth of
the French Alps. Over several ice ages, a natural filter developed, creating a one of a kind
haven for the planets most precious treasure.

Smart water.

Drinking water, including bottled water, may reasonably be expected to contain at least small
amounts of some contaminants. The presence of contaminants does not necessarily indicate
that water poses a health risk. More information about contaminants and potential health effects
can be obtained by calling the United States Food and Drug Administration, Food and Cosmetic
Hotline (1-888-723-3366). Some persons may be more vulnerable to contaminants in drinking
water than the general population. Immuno-compromised persons, including, but not limited to,
persons with cancer who are undergoing chemotherapy, persons who have undergone organ
transplants, persons with HIV/AIDS or other.
Arrowhead.
There are over three hundred fifty-eight million trillion gallons of water on Earth. But not all water
is created There are over three hundred fifty-eight million trillion gallons of water on Earth. But
not all water is created equal. Arrowhead Brand 100% Mountain Spring Water comes only from
carefully selected mountain springs that are continually replenished. What starts out as rain and
snow, soaks into the ground and is filtered naturally by the earth with a distinct composition of
minerals to create our crisp, refreshing taste.

Propel water with electrolytes.

The quality standards of bottled water provide the maximum legal limits for a variety of
substances that are allowed in bottled water, along with their monitoring requirements. The
substances include microbiological contaminants, pesticides, inorganic contaminants, organic
contaminants, radiological contaminants, and others. The standards have been established by
the United States Food and Drug Administration (FDA), based on the public drinking water
standards of the United States Environmental Protection Agency (USEPA). CDPH adopts the
FDA regulations pertinent to the quality standards of bottled water.
Dasani purified water.
In designing DASANI to be the best tasting water, we start with the local water supply, which is
then filtered by reverse osmosis to remove impurities. The purified water is then enhanced with
a special blend of minerals for the pure, crisp, fresh taste thats delightfully DASANI.

Niagara purified drinking water

Our water is tested by requirements that surpass those set in place by the United States Food
and Drug Administration (FDA) or the Environmental Protection Agency, so you know the water
youre drinking is safe and healthy for the family.

Kroger purified bottled water

Minimally processed products usually contain a very short ingredient list. For the most part, they
are comprised of simple ingredients that you would have found in your great grandmother's
kitchen 100 years ago.This product is in the top 10% of the products in its category.Purified
water is filtered tap water. If tap quality is good in your neighborhood, you don't need this.