37 2

2013 3

ACTA HYDROBIOLOGICA SINICA

Vol. 37, No.2

Mar.,

2013

doi: 10.7541/2013.28

(1. , , 235000;
2. , , 230039)

: 2011 3 6 9 12 11 ,
9 26 , , 3 ,
15 , , Pearson
(r = 0.617, P = 0.043, n = 11)
(r = 0.654, P = 0.029, n = 11)(One-way ANOVA), Shannon-Wiener
[F(3, 40) = 0.225, P = 0.879]Margalef [F(3, 40) = 0.902, P = 0.449]Pielou [F(3, 40) = 0.841,
P = 0.479] 3 [F(3,

40)

= 1.931, P = 0.14], [F(3,

40)

5.45, P = 0.003], 6 [(90.13 36.31) ind./m2],

12 [(14.42 2.78) ind./m2]; , 9
[(155.08 50.43) g/m2], 3 [(39.15 14.08) g/m2](One-way ANOSIM)
, , P
0.082 0.514 53.87% 59.12%,
58.76% 68.58%(Redundancy Analysis RDA),

: ; ; ; ;
: Q145+.2

: A

: 1000-3207(2013)02-0367-09

[1]

; ,

[2]

,
[3]

[3]

,
[5]

, [6] [7]

,
,

1.1

, , 1000 km,

2011 3 6 9 12

0.2 190000 km2,

, 11 (1. :

,
,

: 2012-03-29; : 2012-08-05
: (1979), , ; ; E-mail: jianhuad2004@gmail.com
: (1969), , ; ; ; Tel: 0561-3802381, E-mail: dengdg@263. net

368

37

322343N, 1132244E; 2. : 321620N,

(WMP-5,

1140152E; 3. : 321835N, 1144551E; 4.

)pH

: 322547N, 1152315E; 5. : 322521N,

, (Starflow

1153615E; 6. : 323251N, 1163356E;

6526E, Unidata Pty ),

7. : 324641N, 1170550E; 8. :

, GPS

325713N, 1171655E; 9. : 325442N,

1173849E; 10. : 330036N, 1182849E;

3 ,

11. : 321253N, 1183624E)

GPS

, , ,

11 ,

2 ,

, 16 m , ,

( 1)

( 0.07

1.3

1.2

m ) 1

km , 200 m ,

: Shannon-Wiener

H = Pi log 2 ( Pi ) , , Pi i

( 1/4 2/4 3/4 ) 3

, S ;

3 (

Margalef d = ( S 1) / ln N , , S

, N ; Pielou

, )

J = H / log 2 S , , S , N

40 ,

, H Shannon-Wiener

4%,

, ,

PRIMER 5.0 (Plymouth

( 0.0001 g),

routines in multivariate ecological research)

()

SIMPER (Similarity

[1]

percentages) ANOSIM (Analysis of similarities)

,
1
Tab. 1

()

The physical and chemical properties of the water for the mainstream of Huaihe River (Mean SE)

Physical and chemical indexes

Water temperature ()

3
Mar.

6
Jun.

8.60.42a*
a

9
Sep.

25.50.37b

12
Dec.

23.40.68c
a

Depth (cm)

780.9167.4

Width (m)

200.983.1a

202.982.7a

202.082.9a

681.8161.4
7.90.17

7.20.22

pH

7.70.18

Dissolved oxygen (mg/L)

7.140.28a

10.530.43b

Water transparency (cm)

38.412.02a

46.59.12a

Conductivity (s/cm)

599.2100.7

Total dissolved solids (mg/L)

510.289.2a

Salinity ()
Velocity (cm/s)

0.320.05

ab

803.5126.3

23.211.20a

0.400.06
8.42.12a

7.60.15ab

7.410.33a
545.769.0

0.280.03

7.350.27a
24.14.29ab

ab

371.148.8a
b

742.3151.0a
198.583.5a

11.42.75b
b

444.448.3a
ab

860.9288.2

14.00.57d
a

503.428.3a
343.319.6a

ab

25.77.99a

: * (P < 0.05, Duncans test)

Note: * Different superscript letters indicate significant differences among seasons (P < 0.05, Duncans test)

0.250.05a
21.75.12a

369

[8]

log10(x+1),

53.87% 59.12%, 6
, 9 ,

SPSS 13.0

( 3)

One-way ANOVA , 2

58.76% 68.58%,

, log10(x+1)

24 , ( 4)

Pearson

2.3

CANOCO 4.5

log10(x+1)

(Detrended Correspondence Analysis, DCA)

CANOCO 4.5

1 , 2.3,

(Redundancy analysis, RDA),

(Redundancy Analysis, RDA) [9](

5), 0.07,

2.1

, 9 26 (

2), ,

(Monte carlo permutation test)

3 , 15

, (Width)(Depth)

, ,

(Sand)

3 , RDA ( 3)

0,

Pearson
(r =

, [10]

0.617, P = 0.043, n = 11)(r = 0.654,

P = 0.029, n = 11),

(r = 0.550, P = 0.080, n = 11

[11]

r = 0.464, P = 0.151, n = 11)

[12]( [13] pH[14]

( 1)One-way ANOVA

[15])( [16] [17]

, Shannon-Wiener [F(3, 40) =

[15, 18])

0.225, P = 0.879]Margalef [F(3,

40)

0.902, P = 0.449]Pielou [F(3, 40) = 0.841,

[19,

P = 0.479] 3 [F(3, 40) = 1.931, P =

[20] ; ,

0.14], [F(3,

40)

20]

5.45, P = 0.003],

, [21]

6 [(90.13 36.31) ind./m2],

, ,

12 [(14.42 2.78) ind./m ];

, ,

, 9

[(155.08 50.43) g/m ], 3

[22] ,

[(39.15 14.08) g/m2]( 2)

One-way ANOSIM ,

[23],

P 0.082 0.514

2.2

, ,

370

Tab. 2

37

2
The composition and distribution of benthic mollusca in Huaihe River
Sampling sites

Species

Gastropoda
Lymnaeidae
1 Radix ovata

++

++

Melaniidae
2 Semisulcospira cancellata

++

++

++

+++++

++++

++++

++

+++

Viviparidae
3 Bellamya aeruginosa

++

++++

4 B. purificata

5 B. quadrata

Hydrobiidae

6 Alocinma longicornis

7 Bithynia fuchsiana

Lamellibranchia
Corbiculidae
8 Corbicula fluminea

+++++ +++++ ++++ ++++

++

++

+++++

+++

Sphaeriidae
9 Sphaerium lacustre

Mytilidae
10 Limnoperna lacustris

++++

++

+++

++

++

++

+++

+++

Solecurtidae
11 Novaculina chinensis
Unionidae
12 Acuticosta chinensis

++

13 A. ovata

+
+

14 Anodonta woodiana pacifica

+++

+
+

15 A. w. elliptica

+
+

16 Arconaia lanceolata

17 Cuneopsis heudei

18 Hyropsis cumingii

19 Lamprotula bazrni

++

20 L. caveata

++

21 L. rochechouarti

22 Lanceolaria gladiola

23 Lepidodesma languilati

24 Schistodesmus lampreyanus

25 Solenaia oleivora

26 Unio douglasiae

3
2

7
2

10

10

11

15

13

11

10

: Space= 0 ind./m ; + = 01 ind./ m ; ++ = 15 ind./ m ; +++ = 510 ind./ m ; ++++ = 1020 ind./ m ; +++++ >
20 ind./ m2

1
Fig. 1

371

(a)(b)

Spatial variation of density and biomass (a) and river depth and width (b) at each sampling site in the mainstream of Huaihe River
()
Full name of each sampling site can be found in text (Mean SE)

(Shannon-Wiener Margalef Pielou )

()
Fig. 2 Temporal variation of diversity (Shannon-Wiener index, Margalef index and Pielou evenness index), density and biomass of mollusca
in the mainstream of Huaihe River (Mean SE)

, ,
1500 m 28 ,

, ,

2 , 9
,

, [25]
3

DCA (

2)

, 6 ,

Fig. 3 DCA ordination diagrams of mollusca in the mainstream of

Huaihe River between the species and environmental variables
(Full species names are listed in Table 2)

, 1650 cm ,

5 , 6

[24]

, ,
, 9
[26] ,

4 ,

372

37

3 (ind./m2)
Tab. 3 Temporal variation of the mean density (ind./m2 ) of dominant species for each group and the contribution (%) to within group similarity of the mollusca in the mainstream of Huaihe River
3 Mar.

6 Jun.

9 Sep.

12 Dec.

Corbicula fluminea

Dominant species

9.29 (73.07%)

67.98 (66.88%)

14.29 (86.01%)

9.22 (81.89%)

Limnoperna lacustris

2.95 (8.33%)

8.42 (10.46%)

Bellamya aeruginosa

0.98 (5.67%)

2.5 (5.97%)

2.42 (6.63%)

1.3 (4.34%)

Novaculina chinensis

2.36 (5.66%)

2.19 (2.33%)

Cuneopsis heudei

0.71 (3.29%)

Semisulcospira ancellata

0.66 (2.4%)

Total

15.58 (92.73%)

0.69 (5.85%)

82.46 (91.34%)

16.716 (92.64%)

11.21 (92.08%)

4 (g/m2)
Tab. 4 Temporal variation of the mean biomass (g/m2 ) of dominant species for each group and the contribution (%) to within group similarity of the mollusca in the mainstream of Huaihe River
Dominant species
Corbicula fluminea

3 Mar.

6 Jun.

9 Sep.

12 Dec.
54.94 (82.95%)

18.13 (81.18%)

35.17 (70.62%)

55.01 (79.59%)

Bellamya aeruginosa

1.55 (5.25%)

1.21 (6.45%)

5.08 (8.06%)

Cuneopsis heudei

3.75 (4.93%)

12.05 (8.91%)

Lamprotula bazrni

18.28 (8.99%)

Unio douglasiae

3.09 (4.99%)

Total

23.43 (91.36%)

53.67 (5.79%)

57.75 (91.0%)

113.76 (93.44%)

39.99 (91.86%)

5 (Redundancy analysis RDA)

Tab. 5 Results of canonical correspondence analysis
Axes

Eigen values

0.235

0.104

0.091

0.030

- Species-environment correlations

0.891

0.667

0.694

0.656

Total inertia
1.000

Cumulative percentage variance

Species data

23.5

33.9

42.9

42.9

- Species-environment relation

46.5

67.1

85.1

91.0

Test of significance:
1 Axis 1: F = 9.504, P = 0.022
All canonical axes: F = 2.622, P = 0.002
Note: Total inertia is total variance in species abundance data

[25], 9 , 9

, ,

, ,

61.6 g/ind., 9

[28] ,

3 ,

[15]

[11] ,

[1, 27],

, ,

, [28] ,

[29], ,

[30]

373

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375

COMMUNITY STRUCTURE OF BENTHIC MOLLUSCA AND ITS RELATIONSHIP

WITH ENVIRONMENTAL FACTORS IN THE MAINSTREAM OF HUAIHE RIVER
DING Jian-Hua1, ZHOU Li-Zhi2, DENG Dao-Gui1 and JIN Xian-Wen 1
(1. Anhui Key Laboratory of Resource and Plant Biology, School of Life Sciences, Huaibei Normal University,
Huaibei 235000, China; 2. Anhui Key Laboratory of Eco-engineering and Bio-technique,
School of Resources and Environmental Engineering, Anhui University, Hefei 230039, China)

Abstract: The benthic mollusca community structure at eleven sampling sites in Huaihe River was investigated in
March, June, September and December 2011. A total of 26 species from 9 families were recorded. Thereinto, only 3
species occurred in the first upriver site: Tongbaijinzhuang, and 15 species occurred in the downriver site: Xinchengkou.
There were remarkable correlations between water depth and the number of species (r = 0.617, P = 0.043, n = 11), and
water depth and density (r = 0.654, P = 0.029, n = 11). Consequently, the number of species showed a certain increasing trend from upriver to downriver sites. The results of One-way ANOVA showed that there were insignificant seasonal
variations in three biocenotic indices (Shannon-Wiener diversity index [F(3, 40) = 0.225, P = 0.879], Margalef index
[F(3, 40) = 0.902, P = 0.449], and Pielou evenness index [F(3, 40)= 0.841, P = 0.479]) and biomass [F(3, 40) = 1.931, P = 0.14],
but not in density [F(3, 40) = 5.45, P = 0.003]. The mean maximal density [(90.13 36.31) ind./m2] occurred in June while
the minimum [(14.42 2.78) ind./m2] appeared in December. Different with the former, the mean maximal biomass
[(155.08 50.43) g/m2] occurred in September while the minimum [(39.15 14.08) g/m2] appeared in March. The results of One-way ANOSIM showed that there was no remarkable seasonal change in mollusca community structure at
density and biomass composition (both P > 0.05). The average dissimilarity of density between months ranged from
53.87% to 59.12%, while from 58.76% to 68.58% in biomass. Redundancy Analysis (RDA) showed that river width,
water depth and the type of substrate were the main environmental variables deciding the spatial distributions and species richness in Huaihe River.
Key words: Huaihe River; Benthic Mollusca; Community Structure; ANOSIM; RDA